Fig. 2: Lon knockdown flies have altered respiratory chain function and abundance. | Cell Death Discovery

Fig. 2: Lon knockdown flies have altered respiratory chain function and abundance.

From: Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation

Fig. 2

a The activity of respiratory chain complexes in control and LonKD flies at 1 day (left panel) and 21 days (right panel) of age (n = 2 independent groups of 500 adult flies, *p < 0.05, **p < 0.005 from Student’s t-test). b BN-PAGE analysis of mitochondrial protein extracts from 21-day-old control and LonKD flies. The red asterisk marks the location of the subcomplex containing F1 subunit of ATP synthase that is only detected in LonKD flies. Immunoblot of citrate synthase (bottom panel) was used as a loading control. c In-gel activity of mitochondrial complexes I and IV isolated from 21-day-old adults. SCs here refer to the supercomplexes. d In-gel activity of mitochondrial complex II isolated from 21-day-old flies. e In-gel activity of mitochondrial complex V isolated from 21-day-old adults. The red asterisk denotes the location of the subcomplex containing Fsubunit of ATP synthase in LonKD flies. Note that the protein molecular weight markers shown in (b) were used as reference to mark the gels in (c–e). For BN-PAGE and in-gel activity assays, the images shown are representative of two independent biological replicates. Control = UAS-mCherry-RNAi driven by elav-GAL4 and da-GAL4. LonKD = UAS-Lon-RNAi-1 driven by elav-GAL4 and da-GAL4

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