Fig. 1: Inactivation of Lon results in shortened lifespan and defective locomotion. | Cell Death Discovery

Fig. 1: Inactivation of Lon results in shortened lifespan and defective locomotion.

From: Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation

Fig. 1

a Immunoblot analysis from heads of 1-day-old control and LonKD flies using Lon and actin antibodies. The Lon band intensity is normalized against actin as a loading control. Significance was determined using Student’s t-test (***p< 0.0005 by Student’s t-test). The experiment was repeated at least three times. b Kaplan–Meier survival curves of LonKD flies (n = 167, 50% survival 31 days) and controls (n = 174, 50% survival 73 days) (****p < 0.0001 by Mantel–Cox log-rank test). c One-day-old LonKD flies exhibit a climbing defect. Error bars represent SEM (n= 135 for LonKD, 134 for control, ***p < 0.0005 by Student’s t-test). d One-day-old LonKD flies exhibit a significant decrease in flight index (n= 6 independent groups of 10–15 animals, **p < 0.005 from Student’s t-test). Control = UAS-mCherry-RNAi driven by elav-GAL4 and da-GAL4. LonKD = UAS-Lon-RNAi-1 driven by elav-GAL4 and da-GAL4

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