Abstract
Deubiquitinating enzymes (DUBs) regulate numerous cellular functions by removing ubiquitin modifications. We examined the effects of 88 human DUBs on linear ubiquitin chain assembly complex (LUBAC)-induced NF-κB activation, and identified OTUD1 as a potent suppressor. OTUD1 regulates the canonical NF-κB pathway by hydrolyzing K63-linked ubiquitin chains from NF-κB signaling factors, including LUBAC. OTUD1 negatively regulates the canonical NF-κB activation, apoptosis, and necroptosis, whereas OTUD1 upregulates the interferon (IFN) antiviral pathway. Mass spectrometric analysis showed that OTUD1 binds KEAP1, and the N-terminal intrinsically disordered region of OTUD1, which contains an ETGE motif, is indispensable for the KEAP1-binding. Indeed, OTUD1 is involved in the KEAP1-mediated antioxidant response and reactive oxygen species (ROS)-induced cell death, oxeiptosis. In Otud1−/−-mice, inflammation, oxidative damage, and cell death were enhanced in inflammatory bowel disease, acute hepatitis, and sepsis models. Thus, OTUD1 is a crucial regulator for the inflammatory, innate immune, and oxidative stress responses and ROS-associated cell death pathways.
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Introduction
The linear ubiquitin chain assembly complex (LUBAC), composed of HOIL-1L (also known as RBCK1), HOIP (RNF31), and SHARPIN, is an E3 complex that specifically generates the Met1(M1)-linked linear polyubiquitin chain [1, 2]. Upon stimulation by proinflammatory cytokines, such as TNF-α, LUBAC is recruited to the TNF receptor (TNFR), and conjugates M1-ubiquitin chains to NF-κB-essential modulator (NEMO) and receptor-interacting serine/threonine-protein kinase 1 (RIP1). The M1-ubiquitin chain functions as a scaffold to form the TNFR signaling complex I and recruit the canonical IκB kinase (IKK), composed of IKKα, IKKβ, and NEMO [3, 4]. The recruited IKK molecules are activated and leading to the NF-κB signaling. Moreover, the LUBAC is crucial for anti-apoptosis, and genetic defects and polymorphisms of LUBAC are associated with various disorders [2].
At present, the K11-, K63-, and M1-ubiquitin chains are known to be involved in TNFR signaling complex I [4], and deubiquitinating enzymes (DUBs), such as Cezanne (OTUD7B), OTULIN, CYLD, and A20, are reportedly involved in NF-κB regulation [5]. Cezanne [6] and OTULIN [7] selectively hydrolyze K11- and M1-ubiquitin chains, respectively. Moreover, OTULIN and CYLD bind to the N-terminal PUB domain of HOIP, which regulates LUBAC activity [8, 9]. In contrast, A20 does not cleave linear polyubiquitin chains, and instead, it binds to the M1-ubiquitin chain, resulting in NF-κB suppression [10]. However, the DUBs that physiologically regulate LUBAC functions have remained elusive.
In this study, we examined the effects of 88 human DUBs on LUBAC-mediated NF-κB activation, and identified OTUD1 (also known as DUBA7) [11], as the most potent down-regulator. We further identified that OTUD1 is involved not only in TNF-α-induced apoptosis and necroptosis but also in KEAP-1-mediated oxidative stress response and reactive oxygen species (ROS)-induced cell death, oxeiptosis.
Materials and methods
A detailed method is available in the Online Supplemental Materials.
Results
OTUD1 is a negative regulator of canonical NF-κB signaling
To comprehensively explore the DUBs involved in LUBAC-mediated NF-κB activation, we prepared 88 human DUB cDNAs and analyzed their effects by a luciferase assay in HEK293T cells (Fig. 1a). Taking the NF-κB activity induced by the expression of LUBAC alone as 100%, its co-expression with 16 DUBs, such as USP10, upregulated the NF-κB activity. In contrast, 34 DUBs significantly downregulated the LUBAC-induced NF-κB activation. Since OTUD6A, OTUD2, and OTUD1 had stronger inhibitory effects than those of the known LUBAC-suppressive DUBs, such as CYLD, OTULIN, and A20, we performed another NF-κB luciferase assay by expressing 10-fold higher amounts of LUBAC subunits than those in Fig. 1a. As a consequence, these DUBs dose-dependently suppressed the LUBAC- and/or TNF-α-induced NF-κB activation (Fig. 1b, Supplementary Fig. 1a, b). Since OTUD1 showed the most potent inhibitory effect, we focused on investigating its physiological functions.
Human OTUD1 is composed of an unknown N-terminal region (a.a. 1–290), an OTU domain (a.a. 291–446), which contains the active Cys320, and a ubiquitin-interacting motif (UIM, a.a. 457–481) (Fig. 1c) [11]. Although the N-terminal region lacks homology with known domains, it shares similarities with the amino acid sequence of the hypothetical protein Rv1157c of Mycobacterium tuberculosis (Supplementary Fig. 1c). Interestingly, the region contains an abundance of Ala (21.7%), Pro (16.9%), and Gly (9.6%). Therefore, we named the region as Ala-, Pro-, and Gly-rich region (APGR). To identify the critical region of OTUD1 in NF-κB inhibition, we constructed various mutants of OTUD1 and performed luciferase assays (Fig. 1c, d, Supplementary Fig. 1d). The OTUD1-Wt strongly suppressed the LUBAC- and TNF-α-induced NF-κB activities and the catalytically inactive OTUD1-CA showed partial NF-κB suppression. The combined mutation with the catalytically inactive APGR-deletion (ΔAPGR-CA) completely abolished the NF-κB-suppressive effect of OTUD1. These results suggested that both the catalytic activity and the APGR play a role in NF-κB suppression.
The protein disorder prediction by DICHOT [12] suggested that APGR is an intrinsically disordered low complexity domain (Fig. 1e). Moreover, the endogenous and transiently expressed FLAG-tagged OTUD1 (calculated molecular weight: 51 kDa) in HEK293T cells eluted in broad fractions in a gel filtration analysis, mainly at approximately 330–240 kDa (Fig. 1f). In contrast, FLAG-ΔAPGR (calculated molecular weight: 22 kDa) predominantly eluted at monomer fractions of <44 kDa. These results suggested that the APGR functions as a protein-interaction site.
OTUD1 preferentially cleaves K63-linked ubiquitin chain
OTUD1 primarily cleaves K63-linked diubiquitin [11], but it also cleaves K6-, K11- and K48-chains [13, 14]. Furthermore, OTUD1 reportedly cleaves K33-chains in SMAD7 [15], and atypical K6-, K11-, and K29-chains from IRF3 [16]. Our biochemical analyses reconfirmed that the full-length FLAG-OTUD1-Wt in HEK293T cell lysates effectively converted K63-linked diubiquitin to monoubiquitin, among the 8 types of diubiquitins tested in vitro (Supplementary Fig. 1e). Moreover, recombinant GST-OTUD1 completely hydrolyzed the K63-linked polyubiquitin chain, and processed a long K48-linked polyubiquitin chain to tetraubiquitin (Supplementary Fig. 1f). However, OTUD1 did not cleave M1-chains, indicating that OTUD1 downregulates the NF-κB activation independently of the cleavage of linear ubiquitin chain, and principally by hydrolyzing the K63-linked ubiquitin chain.
OTUD1 down-regulates inflammatory cytokine-induced canonical NF-κB activation
The OTUD1 gene is composed of a single exon in both humans and mice, and we constructed OTUD1-knockout (OTUD1−/−) HeLa and HEK293T cells and Otud1−/−-mice (Supplementary Fig. 2a, b). The genetic depletion of OTUD1 showed no effect on the expression of LUBAC and NF-κB signaling factors (Supplementary Fig. 2c). Moreover, OTUD1 is ubiquitously expressed in various mouse tissues (Supplementary Fig. 2d), and intracellular amounts of K48-linked, K63-linked, and pan-ubiquitin chains were not affected by the genetic ablation of Otud1 in mouse embryonic fibroblasts (MEFs) (Supplementary Fig. 2e).
To examine the cellular functions of OTUD1, we analyzed the TNF-α-induced canonical NF-κB pathway. The genetic ablation of OTUD1 in HEK293T cells enhanced the TNF-α-induced NF-κB luciferase activity (Supplementary Fig. 2f). In OTUD1−/−-HeLa cells, TNF-α-induced phosphorylation of IκBα and p65, hallmarks of NF-κB activation, was upregulated as compared to that in the parental cells (Fig. 2a). Moreover, the FLAG-TNF-α precipitation indicated that OTUD1 is recruited to TNFR upon TNF-α stimulation, and the polyubiquitinations of RIP1 and LUBAC in the TNFR signaling complex I were enhanced in OTUD1−/−-cells as compared to the parental cells (Fig. 2a, Supplementary Fig. 2g). TANK-binding kinase 1 (TBK1) and IKKε reportedly prevent TNF-induced cell death by RIP1 phosphorylation [17]. Importantly, the recruitment and phosphorylation of TBK1 and IKKε were reduced in the TNFR-signaling complex I from OTUD1−/− cells (Supplementary Fig. 2h). Thus, OTUD1 reciprocally regulates TNF-α-mediated NF-κB activation and TBK1/IKKε-mediated signaling in HeLa cells. We further investigated the effect of the genetic ablation of OTUD1 on TNF-α-induced K63-ubiquitination, by using a tandem ubiquitin-binding entity (TUBE) [18]. After TNF-α stimulation, the K63-polyubiquitinations of NEMO and RIP1 were enhanced in OTUD1−/−-HeLa cells (Fig. 2b), indicating that OTUD1 regulates K63-ubiquitination in complex I. Indeed, the expression of NF-κB target genes was enhanced in TNF-α-treated OTUD1−/−-HeLa cells (Supplementary Fig. 2i). These results clearly indicated that OTUD1 is a component of TNFR signaling complex I and regulates the K63-ubiquitination of NF-κB activators, such as RIP1, LUBAC, and NEMO, in the TNF-α signaling pathway.
Similarly, upon stimulation with IL-1β, the phosphorylation of NF-κB factors and the subsequent degradation of IκBα were enhanced in OTUD1−/− cells as compared to the parental cells (Fig. 2c). In contrast, the phosphorylation of JNK, a MAP kinase, was not affected in OTUD1−/−-cells. When K63-polyubiquitinated proteins were captured from cell lysates by K63-TUBE, enhanced high molecular weight smear migrations of interleukin-1 receptor-associated kinase 1 (IRAK1), HOIP, and SHARPIN were detected in IL-1β-treated OTUD1−/−-HeLa cells (Fig. 2d). In contrast, the M1-TUBE analysis indicated that the M1-ubiquitination of these factors was not increased (Fig. 2e), suggesting that OTUD1 regulates the IL-1β-induced K63-deubiquitination of IRAK1 and LUBAC. After IL-1β-treatment, the mRNA and protein levels of NF-κB targets were enhanced in OTUD1−/−-cells, as compared to those in parental cells (Supplementary Fig. 2j, k).
To further investigate the effect of OTUD1 on signal transduction and transcription, we performed RNA-seq analysis. A principal component analysis clearly demonstrated that the OTUD1-deficiency affected the transcription of a set of genes (Fig. 2f). The gene ontology analyses revealed the significant upregulation of inflammatory responses and NF-κB-related factors in OTUD1−/−-HeLa cells (Fig. 2g, h, Supplementary Fig. 3a). These results clearly indicated that OTUD1 is a negative regulator for the IL-1β-induced canonical NF-κB activation pathway.
We further examined the role of OTUD1 using splenic B cells from Wt- and Otud1−/−-mice, and confirmed that the Otud1-deficiency did not affect the CD40- or B cell receptor-mediated induction of NF-κB target genes (Supplementary Fig. 3b, c). Furthermore, lymphotoxin β-mediated non-canonical NF-κB activation, which is demonstrated by the intranuclear translocation of p52 and RelB, was not affected in Otud1−/−-MEFs (Supplementary Fig. 3d).
OTUD1 activates IFN antiviral signaling
We next examined the antiviral pathway using Otud1+/+- and Otud1−/−-MEFs. Upon stimulation with poly(dA:dT), which activates the IFN antiviral pathway [19], the phosphorylation of TBK1 and IRF3 and the expression of IRF3-target genes were reduced in Otud1−/−-MEF cells as compared to the Otud1+/+-MEFs (Fig. 3a, b). Similarly, the TLR3 ligand poly(I:C)-induced phosphorylation of IRF3 and the expression of IRF3-targets were decreased in Otud1−/−-bone marrow-derived macrophages (BMDMs) and MEFs (Fig. 3c, d, Supplementary Fig. 4a). Upon infection with Sendai virus (SeV), a single-stranded RNA virus, Otud1−/−-MEFs and -BMDMs showed reduced expression of IRF3-target genes as compared to Otud1+/+-cells (Fig. 3e, f). Indeed, RNA-seq analysis of poly(I:C)-treated MEFs revealed that the Otud1-deficiency downregulated the transcription of a set of IFN genes (Fig. 3g, h), and gene ontology analyses demonstrated that the Otud1-deficiency affected the expression of IFN and the RIG-I-like receptor signaling pathway (Fig. 3i, Supplementary Fig. 4b). Collectively, these results indicated that OTUD1 is a positive regulator for the type I IFN antiviral pathway.
OTUD1 suppresses TNF-α-induced apoptosis and necroptosis
When TNFR signaling complex I fail to induce NF-κB activation, TNF-α stimulation induces apoptosis with the formation of complex II, which is composed of Fas-associated death domain protein (FADD) and caspase 8 [20, 21]. A pan-caspase inhibitor (ZVAD) suppresses apoptosis, whereas it induces another programmed necrosis, necroptosis, with the formation of necrosome by RIP1, RIP3, and then mixed lineage kinase domain-like pseudokinase (MLKL) [22].
After an 8 h treatment with TNF-α + cycloheximide (TC), the trypan blue-positive dead cells were significantly increased in OTUD1−/−-HeLa and HEK293T cells (Fig. 4a). The cell survival analysis indicated that OTUD1−/−-HEK293T cells are more sensitive to cell death than the parental cells after TC-treatment (Fig. 4b). Indeed, the cleavages of PARP, caspase 3, and caspase 8, hallmarks of the apoptosis, were enhanced in TC-treated OTUD1−/−-HEK293T cells as compared to those in parental cells (Fig. 4c, d). The effective complex II formation, as shown by the enhanced association of caspase 8 with FADD, was detected in OTUD1−/−-HEK293T cells (Fig. 4d). These results suggested that OTUD1 has an inhibitory effect on the TNF-α-induced apoptosis.
We next investigated the involvement of OTUD1 in necroptosis using MEFs. In the presence of TC + ZVAD (TCZ), the Otud1−/−-MEFs died more rapidly than the Otud1+/+-MEFs (Fig. 4e). The phosphorylation of MLKL and RIP3 was enhanced in Otud1−/−-MEFs (Fig. 4f), and TCZ-induced cell death in Otud1−/−-MEFs was rescued by a RIP1 kinase inhibitor, necrostatin-1 [23] (Fig. 4g). Interestingly, the polyubiquitination of RIP3 was enhanced in Otud1−/−-MEFs (Fig. 4h, left panel), indicating that RIP3 is a possible endogenous substrate of OTUD1. When K63-ubiquitinated proteins were pulled down by K63-TUBE, the smeared migration of RIP1 was slightly suppressed upon stimulation with TCZ in Otud1−/−-MEFs (Fig. 4h, middle panel). In contrast, the M1-ubiquitination of RIP3 was not detected in either MEFs (Fig. 4h, right panel). These results indicated that OTUD1 is a critical regulator in TNF-α-mediated necroptosis pathways through the removal of K63-ubiquitin chains from RIP3 under steady-state conditions.
OTUD1 regulates KEAP1-mediated oxidative stress response and cell death
To further examine the physiological roles of OTUD1, proteins interacting with FLAG-OTUD1 in HEK293T cells were analyzed by immunoprecipitation-mass spectrometry. Then, we identified KEAP1 as the most prominent interactor of OTUD1 (Fig. 5a, Supplementary Fig. 5a). KEAP1 regulates the proteasomal degradation of the NRF2 transcription factor, as a component of the Cullin 3 (CUL3)–E3 complex [24]. Moreover, KEAP1 is involved in excess reactive oxygen species (ROS)-induced cell death, named oxeiptosis, with the apoptosis-inducing factor mitochondria associated 1 (AIFM1) and the mitochondrial Ser/Thr protein phosphatase (PGAM5) [25]. Indeed, peptides derived from AIFM1, CUL3, and PGAM5 were identified in FLAG-OTUD1 co-precipitates (Fig. 5a, Supplementary Fig. 5a). We detected the endogenous association of KEAP1 and OTUD1 (Fig. 5b). Gel filtration followed by an immunoblotting analysis showed that KEAP1 eluted in the ~410–330 kDa fractions in HEK293T cells (Fig. 5c), and as shown in Fig. 1f, OTUD1 co-eluted with KEAP1 in the 330 kDa fraction. In contrast, KEAP1 eluted in the ~280 kDa fraction in OTUD1−/− cells. These results clearly indicated that the OTUD1-KEAP1 interaction exists under physiological conditions. Interestingly, the K63-polyubiquitination of KEAP1 was enhanced in Otud1−/−-MEFs (Fig. 5d). We further confirmed the K63-polyubiquitination of KEAP1 in OTUD1−/−-HEK293T cells by using an HA-tagged only-K63-ubiquitin mutant and a K63-ubiquitin-specific antibody (Supplementary Fig. 5b, 5c). Co-expression of OTUD1-Wt, but not OTUD1-CA, suppressed the K63-ubiquitination of KEAP1, suggesting that OTUD1 targets KEAP1 that removes the K63-ubiquitin chain. Moreover, we determined that the APGR of OTUD1 interacts with the C-terminal Kelch and CTR of KEAP1 (Fig. 5e, f). Importantly, we found that the human and mouse OTUD1 proteins contain ETGE and a similar DTGE sequence, respectively, in APGR, which plays the KEAP1-binding motif in NRF2 [26] (Fig. 5g). Indeed, the deletion of the ETGE motif from OTUD1 abolished the KEAP1-binding ability (Fig. 5h). Upon H2O2-treatment, ROS production was upregulated in Otud1−/−-MEFs as compared to Otud1+/+-MEFs (Fig. 5i), resulting in the enhanced expression of antioxidant genes at the early phase (~3 h) (Fig. 5j). However, prolonged treatment with H2O2 caused drastic cell death in Otud1−/−-MEFs, suggesting that the excessive ROS production subsequently induces oxeiptosis (Fig. 5k).
Interestingly, the restoration of OTUD1-Wt, but not the catalytically inactive mutant, rescued ROS resistance and cell viability (Fig. 5l, m), suggesting that the DUB activity of OTUD1 is necessary to resist oxeiptosis. OTUD1 directly bound with KEAP1, and PGAM5 co-precipitated with OTUD1 only in the presence of KEAP1 (Supplementary Fig. 5d). Although the co-precipitation of endogenous AIFM1 with FLAG-OTUD1 was detected by the MS analysis (Fig. 5a, Supplementary Fig. 5a), the co-precipitation of exogenous OTUD1 with AIFM1 was not detected (Supplementary Fig. 5d). Whereas, in Otud1−/−-MEFs, increased intranuclear AIFM1 was detected concomitantly with the reduced mitochondrial AIFM1 (Supplementary Fig. 5e), which may affect mitochondrial oxidative phosphorylation and ROS generation [27]. These results indicated that OTUD1 binds and regulates the K63-ubiquitination of KEAP1 under basal conditions and is important for the efficient antioxidative stress response, ROS production, and oxeiptosis. The increased H2O2-mediated ROS-production and reduced cell viability in Otud1−/−-MEFs than that in Otud1+/+-MEFs were prevented by broad antioxidant scavengers, such as N-acetylcysteine (NAC) and butylated hydroxyanisole (BHA), but not by a mitochondria-targeted superoxide dismutase mimetic, Mito-TEMP (Supplementary Fig. 5f, g). These results suggested that cytosolic ROS scavengers ameliorate the increased ROS levels and cell death in Otud1-deficient cells.
In addition to H2O2 treatment, the ROS levels were also upregulated in Otud1−/−-MEFs upon apoptotic (TC) and necroptotic (TCZ) stimuli, although TNF-α alone showed no effects on ROS production (Supplementary Fig. 5h). Since the enhanced ROS production was canceled by the restoration of Otud1-Wt, but not -CA, in TC- or TCZ-treated Otud1−/−-MEFs, the catalytic activity of OTUD1 is necessary to regulate TNF-α-induced cell death pathways (Supplementary Fig. 5i). Collectively, these results indicated that OTUD1 activity regulates TNF-α-induced apoptosis and necroptosis, as well as KEAP1-mediated oxeiptosis.
OTUD1 suppresses inflammatory and oxidative stress responses in vivo
A recent report showed that OTUD1 inhibits colonic inflammation in vivo [28]. We confirmed that significant shortening of the colon, and higher diarrhea and fecal blood scores were observed in a DSS-administrated ulcerative colitis-like inflammatory bowel disease (IBD) model of Otud1−/−-mice (Supplementary Fig. 6a, b). The middle and distal colon regions in DSS-treated Otud1−/−-mice showed more severe damage, characterized by multiple focal dropouts of entire crypts, inflammatory cell infiltration, and edema, as compared with the DSS-treated Otud1+/+ mice (Fig. 6a). Histologic scores of colitis were significantly higher in the middle and distal colon of DSS-treated Otud1−/−-mice, as compared with DSS-treated Otud1+/+ mice (Fig. 6b). Moreover, the expressions of NF-κB target genes were upregulated in the distal colon from Otud1−/−-mice (Fig. 6c). Importantly, we detected the increased 8-OHdG-positive oxidative DNA damage and TUNEL-positive cell death in the colon of DSS-treated Otud1−/−-mice (Fig. 6d, e). Furthermore, p65 staining was predominantly observed in the cytoplasm of the colonic epithelial cells and the nuclei of a few inflammatory cells (Supplementary Fig. 6c, Arrows) in the lamina propria of the colonic mucosa in the control mice. Nuclear p65-positive colonic epithelial cells (Supplementary Fig. 6c, Arrowheads) were observed in the remaining colonic mucosa of DSS-treated Otud1+/+- and Otud1−/−-mice. Moreover, increased numbers of nuclear p65-positive colonic epithelial cells and inflammatory cells in the DSS-treated Otud1−/−-mice were consistent with the severity of colitis. These findings indicated that the canonical NF-κB activation is increased in DSS-induced colitis in the Otud1−/−-mice, as compared with the Otud1+/+-mice. In contrast, we did not detect any drastic effects on K63-, K48- and pan-ubiquitination in the colonic lysates from Otud1+/+- and Otud1−/−-mice, with or without DSS-treatment (Supplementary Fig. 6d). DSS-treated Otud1−/−-mice also showed splenomegaly with increased TUNEL-positive splenocytes (Supplementary Fig. 6e, f). These results indicated that OTUD1 suppresses the NF-κB-mediated inflammatory and ROS-induced oxidative damage responses, as well as cell death through specific deubiquitination in an in vivo colitis model.
To further clarify the pathophysiological role of OTUD1 in vivo, we adopted LPS/GalN-induced acute hepatitis and LPS-induced sepsis models, which are associated with inflammatory and oxidative responses [29,30,31]. The LPS/GalN-treated Otud1−/−-mice showed significantly shorter survival and remarkably increased hepatocellular death with an enhanced necroptosis score (Fig. 6f, g, Supplementary Fig. 7a, b). NF-κB p65 was negative (score 0) in hepatocytes from Otud1+/+- and Otud1−/−-mice. In contrast, positive p65 staining was observed in the nuclei of the hepatocytes in the LPS/GalN-treated Otud1+/+- (score 1) and Otud1−/−- (score 2) mice (Fig. 6h, Supplementary Fig. 7c). The intranuclear localization of p65 was significantly increased in the hepatocytes of the LPS/GalN-treated Otud1−/−-mice in comparison with the LPS/GalN-treated Otud1+/+-mice, as evidenced by the findings that the canonical NF-κB activation was significantly increased in the LPS/GalN-treated Otud1−/−-mice. Moreover, caspase 3 activation was upregulated in the LPS/GalN-treated Otud1−/−-mice (Fig. 6i). These results indicated that OTUD1 protects against acute hepatitis through the suppression of NF-κB activation and cell death in vivo.
In contrast to LPS/GalN, LPS challenge alone induces systemic inflammation and sepsis with increases in oxidative stress and NF-κB activation [32, 33]. When mice were intraperitoneally administered LPS, we found that the survival of Otud1−/−-mice was significantly shorter as compared to that of Otud1+/+-mice (Supplementary Fig. 7d). Histopathological findings in the livers are shown in Supplementary Table 1 and Supplementary Fig. 7e. In male mice, mild fatty changes characterized by increased microvacuoles in the cytoplasms of hepatocytes were observed in all LPS-treated Otud1+/+- and Otud1−/−-mice, but not in the Otud1+/+ and Otud1−/− controls (Supplementary Fig. 7e, upper panels). In the female mice, the incidence of hepatocellular death (necrosis and/or apoptosis), predominantly localized in the midzonal area, was increased in the LPS-treated Otud1−/−-mice as compared with the LPS-treated Otud1+/+-mice, albeit without statistical significance (Supplementary Table 1, Supplementary Fig. 7e, lower panels). This suggested that the female Otud1−/−-mice exhibited higher susceptibility to LPS-induced hepatotoxicity than their wild-type counterparts. Indeed, the AST activities were significantly elevated in LPS-treated Otud1−/−-mice, as compared to the LPS-treated Otud1+/+-mice (Supplementary Fig. 7f). These results indicated that the genetic ablation of Otud1 enhances septic shock.
OTUD1 is associated with prognosis of kidney cancer
Finally, to investigate the involvement of OTUD1 in human diseases, we analyzed cancer databases. We found that low levels of OTUD1 expression are significantly associated with a poor prognosis in renal clear cell carcinoma patients (Fig. 7a). Importantly, the expressions of OTUD1 and KEAP1 were significantly correlated in the normal kidney cortex; however, the positive correlation was lost in the tumor (Fig. 7b). In contrast, the expression levels of OTUD1 and NF-κB1 were correlated in both the normal and tumor kidney cortexes. Thus, we concluded that the orchestration of OTUD1 with KEAP1 functions to suppress kidney cancer in humans, and therefore, OTUD1 is a critical regulator to maintain homeostasis.
Discussion
OTUD1 was initially found as a biomarker of thyroid cancer [34] that reportedly stabilizes the p53 tumor suppressor [35]. OTUD1 also upregulates the expression of p21 and Mdm2, thus accelerating apoptosis [35], and cleaves K33-linked polyubiquitin chains from the TGF-β pathway inhibitor SMAD7 [15]. Furthermore, OTUD1 is induced by RNA viruses, which gives rise to the upregulation of Smurf1 [36]. OTUD1 reportedly regulates the Hippo pathway [37]. Furthermore, quite recently OTUD1 was found to suppress IBD by removing the K63 ubiquitin chain from RIP1 [28]. Collectively, these findings indicate that OTUD1 functions as a crucial regulator of cancer progression, antiviral host defense responses, and inflammatory responses.
We characterized the APGR as a disordered region (Fig. 1). APGR is required to suppress canonical NF-κB activation, exhibits a broad elution profile in gel filtration, and possesses a KEAP1-interaction motif (Figs. 1 and 5). Therefore, APGR is indispensable for the physiological function of OTUD1. We further found that OTUD1 is a component of TNFR complex I, and the K63-polyubiquitinations of various canonical NF-κB-signaling factors, such as IRAK1, LUBAC subunits (HOIP and SHARPIN), NEMO, and RIP1, were increased upon inflammatory cytokine-stimulation in OTUD1-knockout cells, suggesting that these proteins are potential substrates of OTUD1 (Supplementary Fig. 2 and Figs. 2, 7c).
Although the TNF-α-induced NF-κB activation was upregulated in OTUD1−/−-cells, the activation of TBK1 and IKKε were suppressed (Supplementary Fig. 2), and in contrast to the previous report [16], we determined that IRF3-mediated IFN antiviral signaling was down-regulated by the deficiency of Otud1 (Fig. 3). Since TBK1 activation and IRF3 phosphorylation were suppressed in Otud1−/−-MEFs, OTUD1 seems to be primarily involved in the upstream TBK1/IKKε activation in the type I IFN production pathway (Fig. 7c).
DUBs such as CYLD [38], A20 [39], and OTUB1 [40] are reportedly involved in the regulation of the K63-ubiquitination of RIP1/RIP3. In this study, we showed that the genetic deficiency of OTUD1 accelerated the TNF-α-induced apoptosis and necroptosis, and the basal K63 ubiquitination of RIP3 was enhanced in Otud1−/−-MEFs (Figs. 4, 7c), suggesting that RIP3 is an endogenous substrate of OTUD1 under physiological conditions.
Importantly, we identified OTUD1 as a physiological interactor with KEAP1 through the ETGE motif in APGR (Figs. 5, 7c). KEAP1 senses ROS and protects against oxidative stress as a component of the E3 complex to induce the proteasomal degradation of NRF2 [24]. The DLG and ETGE motifs in the Neh2 domain of NRF2 are crucial for KEAP1-binding [26], and the C-terminal Kelch domain of KEAP1 binds NRF2 as well as OTUD1. We showed that the K63-ubiquitination of basal KEAP1 was upregulated in Otud1−/−-MEFs, indicating that OTUD1 is a physiological regulator of KEAP1. At present, several E3s, such as TRAF6 [41], TRIM25 [42], CKIP1, and SMURF1 [43], reportedly ubiquitinate NRF2-KEAP1. Therefore, OTUD1 may antagonize these E3s.
In the presence of excess amounts of ROS, KEAP1 induces cell death named oxeiptosis through binding with PGAM5 and AIFM1 [25]. Moreover, upon TNF-α-induced necroptosis, PGAM5 binds necrosomes, composed of RIP1–RIP3–MLKL, which mediate the activation of the mitochondrial fission factor Drp1 and mitochondrial fragmentation [44]. A recent report found that OTUD1 is involved in caspase-dependent and -independent apoptosis by facilitating the intranuclear translocation of AIFM1 and the degradation of MCL1 [27]. We showed that OTUD1 is a regulator of the KEAP1–PGAM5–AIFM1 axis, which affects ROS generation and cell death pathways upon oxidative and inflammatory responses (Figs. 5, 7c and Supplementary Fig. 5).
NF-κB and NRF2/KEAP1 are redox-sensitive transcription factors that cooperate in oxidative stress and inflammatory responses and cell death pathways [45]. The intracellular levels of ROS, such as hydrogen peroxide, are regulated by the activities of thioredoxin reductase 1 and glutathione disulfide reductase [46, 47], and OTUD1 seems to be an additional crosstalk regulator of the NF-κB and NRF2/KEAP1 pathways. Furthermore, TNF-α reportedly induces ROS production through the activation of NADPH oxidase 1 complex (Nox1) [48], and RIP1 is necessary for ROS generation at the initiation of caspase-independent cell death [49, 50]. In this study, we showed the drastic increase in ROS levels by the TC- and TCZ-treatments, which accompanied the marked cell death in Otud1−/−-MEFs (Fig. 7c, Supplementary Fig. 5). Free radicals induce oxidative damage, which causes genotoxicity, oxidations, and cell death. Together, KEAP1-NRF2 and NF-κB co-operate the oxidative stress and inflammatory responses, and dysfunctions of these systems are associated with various disorders, such as neurodegeneration and inflammatory diseases [51, 52]. This study has demonstrated that OTUD1 is a central regulatory factor in these signal pathways, and the genetic ablation of OTUD1 is associated with inflammatory bowel disease, hepatitis, sepsis, and kidney cancer (Figs. 6, 7 and Supplementary Figs. 6, 7) [16, 28]. Therefore, OTUD1 is a critical drug discovery target for the treatment of these diseases.
Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Iwai K, Fujita H, Sasaki Y. Linear ubiquitin chains: NF-κB signalling, cell death and beyond. Nat Rev Mol Cell Biol. 2014;15:503–8.
Oikawa D, Sato Y, Ito H, Tokunaga F. Linear ubiquitin code: its writer, erasers, decoders, inhibitors, and implications in disorders. Int J Mol Sci. 2020;21:3381.
Rahighi S, Ikeda F, Kawasaki M, Akutsu M, Suzuki N, Kato R, et al. Specific recognition of linear ubiquitin chains by NEMO is important for NF-κB activation. Cell 2009;136:1098–109.
Haas TL, Emmerich CH, Gerlach B, Schmukle AC, Cordier SM, Rieser E, et al. Recruitment of the linear ubiquitin chain assembly complex stabilizes the TNF-R1 signaling complex and is required for TNF-mediated gene induction. Mol Cell. 2009;36:831–44.
Lork M, Verhelst K, Beyaert R. CYLD, A20 and OTULIN deubiquitinases in NF-κB signaling and cell death: so similar, yet so different. Cell Death Differ. 2017;24:1172–83.
Mevissen TET, Kulathu Y, Mulder MPC, Geurink PP, Maslen SL, Gersch M, et al. Molecular basis of Lys11-polyubiquitin specificity in the deubiquitinase Cezanne. Nature 2016;538:402–5.
Keusekotten K, Elliott PR, Glockner L, Fiil BK, Damgaard RB, Kulathu Y, et al. OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin. Cell 2013;153:1312–26.
Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, et al. Molecular basis and regulation of OTULIN–LUBAC interaction. Mol Cell. 2014;54:335–48.
Wagner SA, Satpathy S, Beli P, Choudhary C. SPATA2 links CYLD to the TNF-α receptor signaling complex and modulates the receptor signaling outcomes. EMBO J. 2016;35:1868–84.
Tokunaga F, Nishimasu H, Ishitani R, Goto E, Noguchi T, Mio K, et al. Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulation. EMBO J. 2012;31:3856–70.
Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, Arnaudo N, et al. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. Cell 2013;154:169–84.
Fukuchi S, Hosoda K, Homma K, Gojobori T, Nishikawa K. Binary classification of protein molecules into intrinsically disordered and ordered segments. BMC Struct Biol. 2011;11:29.
Ritorto MS, Ewan R, Perez-Oliva AB, Knebel A, Buhrlage SJ, Wightman M, et al. Screening of DUB activity and specificity by MALDI-TOF mass spectrometry. Nat Commun. 2014;5:4763.
Takahashi H, Yamanaka S, Kuwada S, Higaki K, Kido K, Sato Y, et al. A human DUB protein array for clarification of linkage specificity of polyubiquitin chain and application to evaluation of its inhibitors. Biomedicines 2020;8:152.
Zhang Z, Fan Y, Xie F, Zhou H, Jin K, Shao L, et al. Breast cancer metastasis suppressor OTUD1 deubiquitinates SMAD7. Nat Commun. 2017;8:2116.
Zhang Z, Wang D, Wang P, Zhao Y, You F. OTUD1 negatively regulates type I IFN induction by disrupting noncanonical ubiquitination of IRF3. J Immunol. 2020;204:1904–18.
Lafont E, Draber P, Rieser E, Reichert M, Kupka S, de Miguel D, et al. TBK1 and IKKepsilon prevent TNF-induced cell death by RIPK1 phosphorylation. Nat Cell Biol. 2018;20:1389–99.
Hjerpe R, Aillet F, Lopitz-Otsoa F, Lang V, England P, Rodriguez MS. Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities. EMBO Rep. 2009;10:1250–8.
Tan X, Sun L, Chen J, Chen ZJ. Detection of microbial infections through innate immune sensing of nucleic acids. Annu Rev Microbiol. 2018;72:447–78.
Cabal-Hierro L, Lazo PS. Signal transduction by tumor necrosis factor receptors. Cell Signal. 2012;24:1297–305.
Zamaraev AV, Kopeina GS, Zhivotovsky B, Lavrik IN. Cell death controlling complexes and their potential therapeutic role. Cell Mol Life Sci. 2015;72:505–17.
Chan FK, Luz NF, Moriwaki K. Programmed necrosis in the cross talk of cell death and inflammation. Annu Rev Immunol. 2015;33:79–106.
Degterev A, Huang Z, Boyce M, Li Y, Jagtap P, Mizushima N, et al. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat Chem Biol. 2005;1:112–9.
Baird L, Yamamoto M. The molecular mechanisms regulating the KEAP1–NRF2 pathway. Mol Cell Biol. 2020;40:e00099–00020.
Holze C, Michaudel C, Mackowiak C, Haas DA, Benda C, Hubel P, et al. Oxeiptosis, a ROS-induced caspase-independent apoptosis-like cell-death pathway. Nat Immunol. 2018;19:130–40.
Tong KI, Katoh Y, Kusunoki H, Itoh K, Tanaka T, Yamamoto M. Keap1 recruits Neh2 through binding to ETGE and DLG motifs: characterization of the two-site molecular recognition model. Mol Cell Biol. 2006;26:2887–2900.
Luo Q, Wu X, Zhao P, Nan Y, Chang W, Zhu X, et al. OTUD1 activates caspase-independent and caspase-dependent apoptosis by promoting AIF nuclear translocation and MCL1 degradation. Adv Sci (Weinh). 2021;8:2002874.
Wu B, Qiang L, Zhang Y, Fu Y, Zhao M, Lei Z, et al. The deubiquitinase OTUD1 inhibits colonic inflammation by suppressing RIPK1-mediated NF-κB signaling. Cell Mol Immunol. 2022;19:276–89.
Li F, Miao L, Sun H, Zhang Y, Bao X, Zhang D. Establishment of a new acute-on-chronic liver failure model. Acta Pharm Sin B. 2017;7:326–33.
Li C, Si J, Tan F, Park KY, Zhao X. Lactobacillus plantarum KSFY06 prevents inflammatory response and oxidative stress in acute liver injury induced by D-Gal/LPS in mice. Drug Des Dev Ther. 2021;15:37–50.
Jia YN, Lu HP, Peng YL, Zhang BS, Gong XB, Su J, et al. Oxyresveratrol prevents lipopolysaccharide/d-galactosamine-induced acute liver injury in mice. Int Immunopharmacol. 2018;56:105–12.
Victor VM, De, la Fuente M. Immune cells redox state from mice with endotoxin-induced oxidative stress. Involvement of NF-κB. Free Radic Res. 2003;37:19–27.
Victor VM, Rocha M, De la Fuente M. N-acetylcysteine protects mice from lethal endotoxemia by regulating the redox state of immune cells. Free Radic Res. 2003;37:919–29.
Carneiro AP, Reis CF, Morari EC, Maia YC, Nascimento R, Bonatto JM, et al. A putative OTU domain-containing protein 1 deubiquitinating enzyme is differentially expressed in thyroid cancer and identifies less-aggressive tumours. Br J Cancer. 2014;111:551–8.
Piao S, Pei HZ, Huang B, Baek SH. Ovarian tumor domain-containing protein 1 deubiquitinates and stabilizes p53. Cell Signal. 2017;33:22–29.
Zhang L, Liu J, Qian L, Feng Q, Wang X, Yuan Y, et al. Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome. PLoS Pathog. 2018;14:e1007067.
Yao F, Zhou Z, Kim J, Hang Q, Xiao Z, Ton BN, et al. SKP2- and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity. Nat Commun. 2018;9:2269.
Hitomi J, Christofferson DE, Ng A, Yao J, Degterev A, Xavier RJ, et al. Identification of a molecular signaling network that regulates a cellular necrotic cell death pathway. Cell 2008;135:1311–23.
Wertz IE, O’Rourke KM, Zhou H, Eby M, Aravind L, Seshagiri S, et al. De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-κB signalling. Nature 2004;430:694–9.
Koschel J, Nishanth G, Just S, Harit K, Kroger A, Deckert M, et al. OTUB1 prevents lethal hepatocyte necroptosis through stabilization of c-IAP1 during murine liver inflammation. Cell Death Differ. 2021;28:2257–75.
Li J, Tian M, Hua T, Wang H, Yang M, Li W, et al. Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain. Autophagy. 2021;17:4062–82.
Liu Y, Tao S, Liao L, Li Y, Li H, Li Z, et al. TRIM25 promotes the cell survival and growth of hepatocellular carcinoma through targeting Keap1–Nrf2 pathway. Nat Commun 2020;11:348.
Gong W, Chen Z, Zou Y, Zhang L, Huang J, Liu P, et al. CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys. Free Radic Biol Med. 2018;115:338–50.
Wang Z, Jiang H, Chen S, Du F, Wang X. The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways. Cell 2012;148:228–43.
Sies H, Berndt C, Jones DP. Oxidative stress. Annu Rev Biochem. 2017;86:715–48.
Hirota K, Murata M, Sachi Y, Nakamura H, Takeuchi J, Mori K, et al. Distinct roles of thioredoxin in the cytoplasm and in the nucleus. A two-step mechanism of redox regulation of transcription factor NF-κB. J Biol Chem. 1999;274:27891–7.
Cebula M, Schmidt EE, Arner ES. TrxR1 as a potent regulator of the Nrf2–Keap1 response system. Antioxid Redox Signal. 2015;23:823–53.
Morgan MJ, Kim YS, Liu ZG. TNFα and reactive oxygen species in necrotic cell death. Cell Res. 2008;18:343–9.
Lin Y, Choksi S, Shen HM, Yang QF, Hur GM, Kim YS, et al. Tumor necrosis factor-induced nonapoptotic cell death requires receptor-interacting protein-mediated cellular reactive oxygen species accumulation. J Biol Chem. 2004;279:10822–8.
Festjens N, Vanden Berghe T, Cornelis S, Vandenabeele P. RIP1, a kinase on the crossroads of a cell’s decision to live or die. Cell Death Differ. 2007;14:400–10.
Wardyn JD, Ponsford AH, Sanderson CM. Dissecting molecular cross-talk between Nrf2 and NF-κB response pathways. Biochem Soc Trans. 2015;43:621–6.
Sivandzade F, Prasad S, Bhalerao A, Cucullo L. NRF2 and NF-B interplay in cerebrovascular and neurodegenerative disorders: molecular mechanisms and possible therapeutic approaches. Redox Biol. 2019;21:101059.
Acknowledgements
We thank Dr. Kohei Nishino (Tokushima University) for MS analysis, Dr. Masaya Fukushi (Hiroshima University) for the generous gift of SeV, Prof. Kohsuke Takeda (Nagasaki University) for helpful discussions on oxeiptosis, Dr. Eiji Goto, Dr. Takanori Abe, Dr. Seigo Terawaki, Ms. Wakana Koeda, Ms. Shiori Motoyama, Ms. Chihiro Yamada, Ms. Yukimi Kira (Osaka Metropolitan University), and Dr. Yuki Katayama (Gunma University) for technical assistance, and the Research Support Platform of Osaka Metropolitan University Graduate School of Medicine for technical assistance. This work was partly supported by AMED under Grant Number JP21gm6410013 (D.O.), and a Grant for Research Program on Hepatitis from the Japan Agency for Medical Research and Development (22fk0210107h001 to F.T.), MEXT/JSPS KAKENHI grants (Nos. JP16H06276 (AdAMS) and JP21H02688 to F.T., JP21K06873, JP21H00291, and JP 20H05337 to D.O., and JP20K16146 to K.S.), JST ACT-X Grant Number JPMJAX2117 (K.S.), and by the Takeda Science Foundation (F.T. and D.O.), Kobayashi Foundation (F.T.), and Joint Usage and Joint Research Programs of the Institute of Advanced Medical Sciences, Tokushima University (F.T.).
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DO, KS, HT, and KK performed biochemical and cell biological experiments, MG performed histochemical analyses, HK conducted mass spectrometric analyses, and MS and SH participated in the mouse analyses. HW, DT, TS, and FT conceived the project. DO, MG, HK, KS, and FT wrote the manuscript and all of the authors participated in commenting on the manuscript.
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Oikawa, D., Gi, M., Kosako, H. et al. OTUD1 deubiquitinase regulates NF-κB- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways. Cell Death Dis 13, 694 (2022). https://doi.org/10.1038/s41419-022-05145-5
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DOI: https://doi.org/10.1038/s41419-022-05145-5