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Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes

Abstract

The balanced functionality of cellular proteostatic modules is central to both proteome stability and mitochondrial physiology; thus, the age-related decline of proteostasis also triggers mitochondrial dysfunction, which marks multiple degenerative disorders. Non-functional mitochondria are removed by mitophagy, including Parkin/Pink1-mediated mitophagy. A common feature of neuronal or muscle degenerative diseases, is the accumulation of damaged mitochondria due to disrupted mitophagy rates. Here, we exploit Drosophila as a model organism to investigate the functional role of Parkin/Pink1 in regulating mitophagy and proteostatic responses, as well as in suppressing degenerative phenotypes at the whole organism level. We found that Parkin or Pink1 knock down in young flies modulated proteostatic components in a tissue-dependent manner, increased cell oxidative load, and suppressed mitophagy in neuronal and muscle tissues, causing mitochondrial aggregation and neuromuscular degeneration. Concomitant to Parkin or Pink1 knock down cncC/Nrf2 overexpression, induced the proteostasis network, suppressed oxidative stress, restored mitochondrial function, and elevated mitophagy rates in flies’ tissues; it also, largely rescued Parkin or Pink1 knock down-mediated neuromuscular degenerative phenotypes. Our in vivo findings highlight the critical role of the Parkin/Pink1 pathway in mitophagy, and support the therapeutic potency of Nrf2 (a druggable pathway) activation in age-related degenerative diseases.

Introduction

Mitochondria are very dynamic organelles as they change their shape, size, and sub-cellular localization depending on the versatile cellular demands for adequate ATP production, optimal cellular function, and survival [1]. A common feature of neurodegenerative conditions such as Parkinson’s (PD) and Alzheimer’s (AD) disease is mitochondria dysfunction [2, 3]. PD is the second most common neurodegenerative disease, characterized by loss of dopaminergic (DA) neurons, rigidity, bradykinesia, postural instability, and tremor [4].

The maintenance of cellular mitostasis directly correlates with mitochondria quality control, which is driven by mitochondrial dynamics, mitochondrial removal, and biogenesis. A terminal response to mitochondria dysfunction is their selective destruction by mitophagy [5]; a highly conserved process, where damaged mitochondria are removed by autophagosomes and delivered for degradation to lysosomes [6]. One of the main pathways involved in this process is the Parkin/PTEN-induced putative kinase 1 (Pink1)-mediated mitophagy [6]. Pink1 is activated by auto-phosphorylation and accumulates on the outer mitochondrial membrane (OMM) of dysfunctional mitochondria triggering the recruitment of the E3 ubiquitin ligase Parkin, which then ubiquitinates several proteins of OMM, amplifying a cascade signal that culminates in mitochondrial engulfment by the autophagosome [7,8,9]. Parkin and Pink1 are the two most commonly mutated proteins in autosomal-recessive juvenile Parkinsonism [10, 11]. Mitophagy disruption is also a hallmark of AD [12], further supporting the importance of mitochondria quality control in neurodegeneration.

Reactive oxygen species (ROS) are main by-products of mitochondria functionality [13] and elevated intracellular ROS levels can cause damage to all cellular biomolecules [14]. A master regulator of cellular redox, and also of proteome homeostasis (referred to as, proteostasis), is the nuclear factor erythroid 2 like 2 [Nrf2; a cap “n” collar (cnc)] transcription factor [15]. Key components of the proteostatic pathways are the molecular chaperones and the two main degradation machineries, namely the autophagy lysosome-(ALP) and the ubiquitin proteasome-(UPP) pathways; ALP is mostly involved in the degradation of protein aggregates and damaged organelles [8], while UPP ensures protein synthesis quality control and degradation of short-lived regulatory proteins [16]. The 26S eukaryotic proteasome comprises a 20S core particle being bound to 19S regulatory particles. The 20S proteasome consists of four stacked heptameric rings (two α-type surrounding two β-type) with the caspase‐like (C‐L), trypsin‐like (T‐L), and chymotrypsin‐like (CT‐L) proteasome enzymatic activities located at the β1, β2, and β5 subunits, respectively [17]. Reportedly, proteasomal degradation is particularly important in PD as it degrades insoluble protein inclusions of α-synuclein [18]. Proteasome and mitochondria are highly interdependent, since UPP requires excessive amounts of ATP and the ubiquitinated OMM proteins are degraded by the proteasome [1]. Notably, the declined functionality of UPP and ALP is a hallmark of aging and of several age‐related diseases, including neuromuscular degeneration [19].

Additionally, to its role in redox and proteome homeostasis, Nrf2 also affects several aspects of mitostasis, including mitochondrial biogenesis, fatty acid oxidation, respiration, ATP production, and the structural integrity of the organelle [20]. Our in vivo studies in the fly model have revealed that the Nrf2 Drosophila ortholog, namely cncC (hereafter referred to as cncC/Nrf2), regulates mitochondria biogenesis [21], while disruption of mitochondria dynamics triggers a cncC/Nrf2-dependent proteostatic response [22]. In C. elegans, Nrf2 becomes activated upon inhibition of mitophagy to promote the expression of mitochondrial biogenesis and mitophagy genes [23]; in support, the mitophagic/autophagic adapter protein sequestosome-1 (SQSTM1/p62) is a Nrf2 transcriptional target [23,24,25].

Herein, we exploit Drosophila as a model organism to investigate the functional role of Parkin/Pink1 in regulating mitophagy and proteostatic responses, as well as in suppressing degeneration-like phenotypes at the whole organism level. We found that Parkin (known as park in Drosophila) or Pink1 knock down (KD) increased cell oxidative load and suppressed mitophagy causing neuronal and muscle degeneration. Concomitant to park or Pink1 KD activation of cncC/Nrf2, induced the proteostasis network, suppressed oxidative stress, restored (independently to ref(2)P/p62) mitophagy and largely rescued park or Pink1 KD-mediated neuromuscular degenerative phenotypes.

Materials and methods

Flies’ culture and stocks

Flies’ stocks were maintained at 25 °C, 60% relative humidity on a 12 h light:12 h dark cycle and were fed standard medium (unless otherwise indicated). The transgenic strains w1118 (#5905), UAS Pink1 RNAi (#55886), UAS Pink1 (#51648), UAS park RNAi (#38333), UAS park (#51651), UAS ref(2)P RNAi (#36111), Gal4-Mef2 (#27390), UAS Mito-GFP (#8443), tub-mito-roGFP2-Orp1 (#67673); the nervous system specific Gal4-Elav (#8765) and the dopaminergic neurons Gal4-TH (#8848) were obtained from the Bloomington Stock Center. The double transgenic line UAS Lamp1-GFP, Actin Gal4 was kindly donated by Dr. G. Orso (University of Padova). The UAS cncC/Nrf2, UAS cncC/Nrf2 RNAi and the Tubulin GeneSwitch Gal4 (tubGSGal4) flies were a gift from Prof. D. Bohmann (University of Rochester, NY, USA); the conditional driver tubGSGal4 is ubiquitously activated upon dietary administration of RU486 (320 μΜ). Mito-QC was kindly donated by Prof. A. Whitworth (University of Cambridge, UK). Given that gonads display distinct aging rates and regulation of proteostatic mechanisms as compared to adult somatic tissues [26], in all presented experiments (unless otherwise indicated) referring to adult flies only micro-dissected somatic tissues (head and thorax; equal numbers from mated male and female flies) were analyzed.

Exposure of flies to compounds, locomotion (climbing), and longevity assays

The transgene (Tg) expression inducer RU486 (Sigma Aldrich) was directly added to the flies’ culture medium. Doses and duration of flies’ exposure to compounds are indicated in figure legends. The locomotion (climbing) and longevity assays were done as described previously [27].

Genomic DNA extraction and conventional PCR analyses

To verify the establishment of transgenic flies carrying more than one Tg, genomic DNA from transgenic larvae or flies’ tissues was extracted with the Genomic DNA Kit of Thermo Scientific (#K0512) and conventional PCR was performed. Primers were designed using the primer-BLAST tool (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and were the following:

Valium20-F: ACCAGCAACCAAGTAAATCAAC, Valium20-R: TAATCGTGTGTGATGCCTACC; cncC/Nrf2-F: TGGAATTGGGCACCCATGGCG, cncC/Nrf2-R: AGTTTGAGTACGTCGTTCAACA.

RNA extraction, cDNA synthesis, and quantitative Real Time PCR (Q‐PCR) analyses

Total RNA extraction from Drosophila tissues, cDNA preparation and Real-time Q-PCR were performed as described before [27]. Used primers have been reported before [27]; for the Pink1/park genes the primers were as follows:

Pink1-F: ACAGCTGGTCTACAACATCC, Pink1-R: ACTGTAGGATCTCCGGACTG;

park-F: TTCTGCCGCAATTGTCTGCAGG, park-R: GCATGCAACCGCCATCTCGCTC;

The RpL32/rp49 gene expression was used as a normalizer.

Preparation of tissue protein extracts, immunoblot analyses and detection of protein carbonyl groups

Adult or larvae tissues protein extracts preparation and immunoblotting analyses were performed as described previously [22]. For the detection of protein carbonyl groups, the OxyBlot protein oxidation detection kit (Millipore, s7150) was used.

Measurement of ROS, proteasome, and cathepsins enzymatic activities in tissue extracts

For ROS measurement dissected flies’ tissues were incubated with fluorescent dye CM-H2DCFDA for 30 min at 25 °C in the dark; the assay was performed as per manufacturer’s instructions.

To measure proteasome activities, heads or somatic tissues were lysed on ice, with buffers suitable for 26S proteasome isolation (0.2% Nonidet P-40, 5 mM ATP, 10% glycerol, 20 mM KCl, 1 mM EDTA, 1 mM DDT, and 20 mM Tris, pH 7.6). Following centrifugation at 19,000×g (4 °C), supernatants (20 μg of protein) were used to measure (after 30 min at 37 °C) the CT-L (LLVY) and C-L (LLE) enzymatic activities by recording the hydrolysis (excitation, 350 nm; emission, 440 nm) of the fluorogenic peptides N-Succinyl-Leu-Leu-Val-Tyr-AMC (AMC = 7-amino-4-methylcoumarin) (BML-P802) and Z-Leu-Leu-Glu-AMC (BML-ZW9345) (Enzo Life Sciences; Farmingdale, NY, USA), respectively.

To measure cathepsins activity, heads or somatic flies’ tissues were homogenized in extraction buffer (1 mM dithiothreitol and 1 M Tris, pH 4.0) and the lysates were cleared at 19,000 × g for 15 min (4 °C). Twenty micrograms of protein were then incubated in the reaction buffer (50 mM sodium acetate, 8 mM cysteine-hydrochloride, 1 mM EDTA, pH 4.0) containing the substrate Z-Phe-Arg-AMC (BML-P139) (Enzo Life Sciences) for 30 min at 37 °C and the fluorescence was recorded (excitation, 350 nm; emission, 440 nm). Assays were performed in adult flies; equal numbers of male and female flies were used.

Tissue preparations for immunohistochemistry and confocal laser scanning microscope (CLSM) imaging

Tissues were collected and dissected in PBS, fixed in 4% formaldehyde for 15 min, washed in PBS containing 0.3% Triton X-100 and incubated with primary antibody overnight at 4 °C. Secondary antibodies, DAPI (for nuclear visualization) or Phalloidin (for actin visualization) staining were applied for 1 h at RT and samples were mounted for viewing. Following dissection tissues were also stained with 100 μΜ LysoTracker Red (as per manufacturer’s instructions). The protocol used for the detection, imaging and processing of oxidized mitochondria was as described before [22]. Samples were viewed in a Digital Eclipse Nikon C1 (Nikon, Melville, NY, USA) CLSM equipped with 40 × 1.0 NA differential interference contrast (DIC), and 60 × 1.4 NA DIC Plan Apochromat objectives; image capturing was done using the EZC1 acquisition and images were analyzed with the CLSM software (Nikon Inc.). Z-stacks with a step size of 0.4 μm were taken using identical settings. Each stack consisted of 28–30 plane images; ~10 animals per genotype were analyzed and representative images are shown. ImageJ JACoP plugin was used for estimating co-localization of lysosomes (Lamp1-GFP) and acidic vesicles stained with LysoTracker Red.

Antibodies and probes

Primary antibodies against the Drosophila proteasome subunits 20S-α (sc-65755), p42A/Rpn7 (sc-65750) and p54/Rpn11 (sc-65746); the anti-Ubiquitin (Ub) (sc-8017) and the HRP-conjugated secondary antibodies were purchased from Santa Cruz. Primary antibodies against the mitochondrial proteins ATP5a/blw (ab14748) and Ndusf3 (ab14711) were purchased from Abcam. Anti-Gapdh (G9545) and anti-Flag (F3165) antibodies were obtained from Sigma Aldrich. The anti-Rabbit-IgG Rhodamine (TRITC) conjugated antibody (711-025-152) and Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (111-545-003) were from Jackson ImmunoResearch. DAPI (D1306), LysoTracker® Red (L7528) and Phalloidin (P3457) were from Molecular ProbesTM-Thermo Fisher Scientific. Anti-Tyrosine Hydroxylase (anti-TH, AB152) was purchased from Merck Millipore and the Gabarap (the fly ortholog of Atg8a; hereafter referred to as Atg8a/Gabarap) antibody (#13733) was from Cell Signaling Technology, Inc.

Mitochondria isolation and measurement of mitochondrial respiration

Mitochondria were isolated as described before [22]. The protein content of isolated mitochondria was measured by the Bradford method and mitochondrial respiration was determined using a Clark-type oxygen electrode connected to a computer-operated Oxygraph control unit (Hansatech Instruments, Norfolk, UK) as described [22]. The respiratory control ratio (RCR) was calculated as the ratio of state 3 to state 4 (ST3:ST4).

Statistical analyses

Experiments were performed at least in triplicate (for each biological replicate, n ≥ 3). Assays were done after pooling isolated male/female tissues from 10 to 20 flies, unless otherwise stated. For statistical analysis, the GraphPad Prism 7.0, the MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 23.0 for Windows, NY, USA) were used. Statistical significance was evaluated using Student’s t-test. Data points correspond to the mean of the independent experiments and error bars denote standard deviation (SD); significance at P < 0.05 or P < 0.01 is indicated in graphs by one or two asterisks, respectively. Gene expression was plotted vs. the respective control set to 1; in all other cases (unless otherwise indicated) control values were set to 100.

For flies’ survival curves and statistical analyses, the Kaplan–Meier procedure and log-Rank (Mantel-Cox) test were used; significance was accepted at P < 0.05. Statistical analyses for all presented longevity experiments are reported in Supplemental Table S1.

Results

KD of park or Pink1 genes in young flies mobilizes proteostatic responses, increases oxidative load, causes mitochondrial aggregation in flies’ tissues, and accelerates age-related degenerative phenotypes

Initially we studied the functional crosstalk of mitostatic and proteostatic modules in young flies’ tissues, by modulating the expression of park or Pink1 genes. We thus ubiquitously (Gal4Tub) overexpressed or knocked down park or Pink1 in Drosophila. To assess the Tgs overexpression (OE)-mediated molecular responses we initially studied the genomic responses in transgenic flies’ somatic tissues. Gene expression analyses showed similar readouts mainly after park or Pink1 KD; these referred to induction of proteasomal genes, as well as to upregulation of cncC/Nrf2 and the mitophagy receptor ref(2)P (the fly ortholog of mammalian SQSTM1/p62; hereafter referred to as ref(2)P/p62), but not of Atg8a (the LC3 ortholog in Drosophila) (Fig. 1A). Some notable exceptions to similar genomic responses were foxo and mitochondrial genes, which tended to be induced in park KD but were downregulated in Pink1 KD mutant flies (Fig. 1A).

Fig. 1: Induced KD of the mitophagy-related park or Pink1 genes in flies’ tissues mobilizes proteostatic responses.
figure1

A Relative mRNA expression of proteasomal subunits, transcription factors (foxo, cncC/Nrf2), autophagy related (Atg8a, ref(2)P/p62) and mitochondrial genes after inducible OE or KD of (A1) park or (A2) Pink1 genes. B Relative mRNA expression levels of park and Pink1 genes in middle aged (30–34 days old) and aged (44–47 days old) wild type flies vs. young (3–7 days old) flies. C Immunoblot analyses (n = 2) after probing the shown transgenic flies’ tissues protein samples with antibodies against 20S-α proteasomal subunits, total ubiquitin (Ub) and protein carbonylation (dinitrophenol, DNP). D, E Relative (%) 26S chymotrypsin-like (CT-L) and caspase‐like (C‐L) like proteasome activities (D) and ROS levels (E) of the shown transgenic lines. F Relative (%) cathepsins activity. G Lysosome quantification (G1) and CLSM co-visualization of mitochondria (Mito-GFP) and lysosomes (LysoTracker Red) (G2) in adult flies’ muscle tissues after muscle-targeted (Gal4Mef2) KD of park or Pink1 genes. H Immunoblot analyses of (H1) somatic tissues probed with anti-Atg8a/Gabarap (n = 2) and (H2) relative protein quantification (NL non lipidated, L lipidated Atg8a/Gabarap). Flies were exposed to 320 μM RU486 in (A) for 7 days and in (CF) for 25 days. Gene expression in (A, B) was plotted vs. control set to 1 (RpL32/rp49 gene was used as reference). Gapdh probing in (C, H1) was used to demonstrate equal protein loading. Bars, ±SD; n = 3, *P < 0.05; **P < 0.01.

We also found that park and Pink1 are downregulated in middle aged (30–33 days old) and aged (>45 days old) wild type flies (Fig. 1B), and thus their induced ubiquitous or tissue specific KD in young flies mimics an age-related condition. Since deregulation of park and/or Pink1 expression relates to human degenerative diseases [10], we focused our studies on park, Pink1 KD-mediated functional outputs.

In support to gene expression analyses, 20S-α proteasomal proteins were upregulated after park or Pink1 KD (Fig. 1C); yet park KD led to the accumulation of ubiquitinated (Ub) and oxidized [carbonylated (DNP)] proteins (Fig. 1C and Supplemental Fig. 1a). Although similar, these effects were milder in Pink1 RNAi expressing mutant flies (Fig. 1C and Supplemental Fig. 1a). Studies in isolated mitochondria showed that neither park nor Pink1 KD affected overall mitochondrial ubiquitination (not shown). Downstream to these effects, prolonged (25 days) ubiquitous (Gal4Tub) park KD upregulated proteasomal activities (Fig. 1D) and either park or Pink1 KD significantly increased oxidative load in transgenic flies’ somatic tissues (Fig. 1E). Furthermore, by using the redox sensitive GFP (roGFPs) probe to study the in vivo oxidative load in the mitochondria of park and Pink1 KD flies, we found that both transgenic fly lines tended to have increased oxidation load (Supplemental Fig. 1b, c). Also, Pink1 KD enhanced cathepsins activity (Fig. 1F), upregulated lysosomes number (Fig. 1G), and induced Atg8a/Gabarap protein and its lipidated form expression levels (Fig. 1H); the ALP-related effects were marginal in park KD mutant flies (Fig. 1F, G).

Analysis of mitochondria structure after muscle targeted (Gal4Mef2) Tg expression using the Mito-GFP reporter revealed the disruption of the mitochondrial network, and the increased formation of mitochondrial aggregates after park or Pink1 KD (but not their OE; not shown) in both larvae (Fig. 2A1) and adult flies (Fig. 2A2) muscles; notably this genetic intervention did not significantly affect mitochondria length (Supplemental Fig. 1d). We also found the wing posture to be disrupted in young flies after muscle-targeted (not shown) or ubiquitous (Gal4Tub) (Fig. 2B) park or Pink1 KD. This degenerative effect coincided with a significant reduction in locomotion (climbing) activity of the transgenic young flies (Fig. 2C), disruption in the organization of muscle intestine fibers (Fig. 2D) and, especially in the park KD mutant flies, accelerated aging (Fig. 2E and Supplemental Table S1).

Fig. 2: Expression of park or Pink1 RNAi accelerates degenerative age-related phenotypes in transgenic flies.
figure2

A CLSM visualization of muscle mitochondria after muscle targeted (Gal4Mef2) KD of park or Pink1 genes in larvae (A1) and adult flies (A2) muscles; Mito-GFP was used to visualize mitochondria, phalloidin (red) for muscle actin and DAPI (blue) for cell nuclei. B Light stereoscope imaging of wing posture in control [RU486 (−)], park and Pink1 RNAi expressing flies. C Relative locomotion (climbing) activity of 25 days old flies of the shown genotypes after ubiquitous (Gal4Tub) Tg expression. D Intestine muscle staining with phalloidin (red) after park or Pink1 muscle targeted (Gal4Mef2) KD. E Longevity curves of the shown transgenic fly lines after ubiquitous (Gal4Tub) park or Pink1 KD. Log-rank, Mantel-Cox test: control vs. park RNAi P < 0.0001, control vs. Pink1 RNAi P < 0.01. Statistics of the longevity curves are also reported in Supplemental Table S1. Flies were exposed to 320 μM RU486 for 25 days, except otherwise indicated. Bars, ±SD; n = 3, *P < 0.05.

Thus, ubiquitous park or Pink1 KD in young flies impacts on mitostatic, proteostatic and antioxidant machineries causing their extensive readjustment, while sustained park or Pink1 KD results in accumulating mitochondrial aggregates, neuromuscular degenerative phenotypes, and accelerated aging.

Neuronal-specific or muscle-specific park, Pink1 KD disrupts proteostasis and suppresses mitophagy

To assess the effects of park or Pink1 genes expression silencing specifically in the brain, we expressed the park or Pink1 RNAi Tgs in flies’ neuronal tissues (Gal4Elav). The dopaminergic (DA) network in the fly is composed of well-characterized neuron clusters, i.e., the PPM1, PPM2, PPM3, PPL1, PPL2, and VUM [28] and considering that DA neurodegeneration is a major sign of PD we immunoassayed the DA neuronal marker tyrosine hydroxylase (TH) in dissected brains after park or Pink1 KD. Following prolonged (25 days) expression of the Tgs in neuronal tissues, we found a significant decrease of the DA neurons number in the PPL1 cluster (Fig. 3A), which has consistently been reported to be affected in PD fly models [29]. Further, we observed collapsed proteostasis being marked by reduced proteasomal (Fig. 3B) and lysosomal (Fig. 3C) cathepsins activities; elevated ROS levels (Fig. 3D) and a significant decrease in the expression levels of proteostatic/mitostatic genes (with the notable exception of the chaperone Hsp70 gene induction after park KD; Fig. 3E1) and of 20S-α proteasomal proteins (Fig. 3E2) in isolated brains of park or Pink1 KD transgenic flies.

Fig. 3: Targeted KD of park or Pink1 in nervous tissues reduces brain DA neurons, downregulates proteostatic modules activities, induces oxidative stress and disrupts mitophagy.
figure3

A CLSM viewing (projection, Z stack) of whole-mount brains from middle aged flies showing (A1) DA neuron clusters marked with anti-TH immunostaining, and (A2) quantification (vs. control) of DA PPL1 neurons in the brains of park or Pink1 KD transgenic flies. BD Relative (%) chymotrypsin-like (CT-L) and caspase‐like (C‐L) like proteasome activities (B), cathepsins activities (C), and ROS levels (D), in flies’ brain after KD of park or Pink1 genes. E1 Relative mRNA expression of proteasomal subunits, cncC/Nrf2, autophagy-related (Atg8a, ref(2)P/p62), Hsp70 and mitochondria-related genes in transgenic flies of the indicated genotypes. E2 Immunoblot analyses and relative quantification of the shown transgenic flies’ brain samples probed with antibodies against 20S-α proteasomal subunits (n = 2). F CLSM visualization of the Mito-QC reporter GFP and mCherry signal in larvae nervous tissues, and (G) quantification of mitolysosomes after park or Pink1 KD in larvae (upper panel) and adult flies (lower panel) brain. AG Tgs were expressed in nervous tissues (Gal4Elav). Gene expression in (E1) was plotted vs. control set to 1; RpL32/rp49 gene was used as reference. Gapdh probing in (E2) was used to demonstrate equal protein loading. Bars, ±SD; n = 3, *P < 0.05; **P < 0.01.

To investigate the contribution of park and Pink1 proteins in neuronal mitophagy turnover rates we used the Mito-QC reporter; a tandem GFP-mCherry fusion protein of the outer mitochondrial membrane, which turns red due to selective quenching of GFP in the acidic microenvironment of the lysosome. The spectral shift results in “red-only” puncta which mark mitolysosomes [27, 30]; in these assays quantification of mitolysosomes was performed using whole brain regions after CLSM Z-stack acquisition. Mitophagy rates in control larvae were developmentally regulated (not shown) and mitophagy was more evident in live vs. fixed neuronal tissues (Supplemental Fig. 2). Our studies with the Mito-QC reporter in control flies showed that nervous tissues express high basal rates of mitophagy (Fig. 3F) and that park KD suppressed mitophagy, as was evident by the significant reduction (vs. controls) of mitolysosomes in both larvae and adult brains (Fig. 3F, G). Pink1 KD in adult flies (but not in larvae) tended to also reduce mitophagy; yet the effect did not reach statistical significance (Fig. 3F, G). Consistently, neuronal specific KD of park, and to a lesser extent of Pink1, accelerated aging of transgenic flies (Supplemental Fig. 3 and Supplemental Table S1). Similarly to nervous tissues, mitophagy rates in muscles were larvae stage-dependent and more evident in live vs. fixed tissues in control flies (Supplemental Fig. 2), while as in the brain, muscle-targeted (Gal4Mef2) park, or Pink1 KD decreased mitolysosomes number in larvae muscles, with park KD displaying again the stronger phenotype (Supplemental Fig. 4).

Taken together these findings highlight the toxic effects induced in young flies’ neuronal or muscle tissues (likely due to disrupted proteostasis and unbalanced mitophagy) following park or Pink1 KD.

OE of cncC/Nrf2 normalizes [independently to ref(2)P/p62)] proteostatic and mitostatic modules functionality in park or Pink1 KD transgenic flies’ tissues

Given our recent findings showing that cncC/Nrf2 is a transcriptional regulator of mitochondrial genes [21], we then examined if park and/or Pink1 genes are also regulated by cncC/Nrf2. We found that cncC/Nrf2 OE induced park and Pink1 (along with ref(2)P/p62) expression levels, which were suppressed after cncC/Nrf2 KD (Supplemental Fig. 5). Thus, cncC/Nrf2 is not only a positive regulator of ref(2)P/p62, park, and Pink1 genes expression, but it also maintains their basal expression levels.

Considering that Nrf2 function is impaired in mitochondria-related disorders, including PD [20], and the high oxidative load in park, Pink1 KD flies, we OE cncC/Nrf2 ubiquitously (Supplemental Fig. 6a) (Gal4Tub) in park and Pink1 RNAi expressing flies. Initially, we found that the endogenous cncC/Nrf2 in Drosophila, is expressed at all developmental stages and in most Drosophila tissues (Supplemental Fig. 6b). OE of cncC/Nrf2 in park or Pink1 KD tissues upregulated proteasomal subunits (Prosα7, Prosβ5, and Rpn11), the mitophagy receptor ref(2)P/p62 and Atg8a genes (Fig. 4A). Consistently, it also augmented 20S-α proteasomal proteins expression (Fig. 4B) and proteasomal activities (Fig. 4C); it also reduced ROS levels (Fig. 4D) and mitochondria oxidative load (Supplemental Fig. 7) as compared to transgenic flies solely expressing park and Pink1 RNAi. Remarkably, OE of cncC/Nrf2 in the park or Pink1 KD background also upregulated the expression of mitochondrial genes [i.e., Marf, Drp1, and PGC1-a (the fly ortholog of mammalian PPARGC1A; peroxisome proliferator-activated receptor gamma coactivator 1-alpha)] (Fig. 4E). In addition, cncC/Nrf2 OE upregulated the expression levels of mitochondrial complex V protein, ATP5a/blw [but not of complex I (Ndufs3)] (Supplemental Fig. 8a), and it also rescued mitochondrial respiration defects (Fig. 4F) and flies wing posture (Supplemental Fig. 8b).

Fig. 4: Concomitant to ubiquitous park or Pink1 KD, cncC/Nrf2 OE upregulates UPP, suppresses oxidative stress and restores normal mitochondria respiration rates in transgenic flies’ tissues.
figure4

A Relative mRNA expression levels of proteasomal subunits (Prosα7, Prosβ5, Rpn11), cncC/Nrf2 and autophagy related (Atg8a, ref(2)P/p62) genes after cncC/Nrf2 OE at the park (A1) or Pink1 (A2) KD genetic background. B Immunoblot analyses and relative quantification of indicated transgenic flies’ somatic tissues probed with antibodies against 20S-α proteasomal subunits. C, D Relative (%) 26S chymotrypsin-like (CT-L) and caspase‐like (C‐L) proteasome activities (C), and ROS levels (D), following cncC/Nrf2 OE in park or Pink1 KD flies. E Relative mRNA expression of mitochondrial genes in shown transgenic flies. F Quantitative analysis of isolated mitochondria respiratory capacity at the indicated transgenic fly lines. AF Tgs were induced ubiquitously (Gal4Tub); flies were exposed to 320 μM RU486. Gene expression in (A, E) was plotted vs. control set to 1 (RpL32/rp49 gene was used as reference). Gapdh probing in (B) was used to demonstrate equal protein loading. Bars, ±SD; n = 3, *P < 0.05; **P < 0.01.

We thus investigated whether cncC/Nrf2 OE could revert tissue specific disrupted mitostasis in park or Pink1 KD flies. We found that muscle-targeted (Gal4Mef2) cncC/Nrf2 OE eliminated Mito-GFP aggregates (Fig. 5A, B) and increased lysosomes number (Lysotracker Red staining; Fig. 5C, D) in both park and Pink1 KD larvae. In support, ubiquitous cncC/Nrf2 overexpressing flies in the park or Pink1 KD background had increased cathepsins activities (Fig. 5E and Supplemental Fig. 8c), suggesting ALP stimulation. Moreover, cncC/Nrf2 OE increased co-localization of Atg8a/Gabarap with mitochondria in larvae (not shown) and adult flies’ muscles (Gal4Mef2) (Fig. 5F) suggesting mitophagy stimulation. Consistently, muscle-specific expression of the Mito-QC reporter showed that cncC/Nrf2 OE significantly enhanced (vs. controls) mitophagy in park and Pink1 KD flies (Fig. 5G).

Fig. 5: cncC/Nrf2 OE enhances autophagy/mitophagy turnover rates in park or Pink1 KD flies.
figure5

A CLSM viewing of mitochondria (Mito-GFP), and (B) quantification of mitochondria aggregates after muscle-targeted (Gal4Mef2) expression of the shown Tgs. C Mitochondria (Mito-GFP), lysosomes (LysoTracker Red) co-staining and (D) quantification of lysosomes in the shown flies’ genotypes after muscle-targeted (Gal4Mef2) Tg expression. E Relative (%) cathepsin activity in transgenic flies after ubiquitous (Gal4Tub) park or Pink1 KD and concomitant cncC/Nrf2 OE. F CLSM viewing of mitochondria (Mito-GFP) and Atg8a/Gabarap immunostaining at the muscles (Gal4Mef2 driver) of shown adult transgenic flies. G CLSM visualization of the Mito-QC reporter GFP and mCherry signal at the indicated transgenic larvae wall muscle (Gal4Mef2 driver). Bars, ±SD; n = 3, *P < 0.05; **P < 0.01.

Interestingly, ref(2)P/p62 KD in either Park RNAi, cncC/Nrf2 OE or Pink1 RNAi, cncC/Nrf2 OE transgenic flies did not revert the cncC/Nrf2 OE-mediated elimination of the Mito-GFP aggregates (Supplemental Fig. 9). Also, ref(2)P/p62 KD in solely park or Pink1 RNAi expressing flies tended to further increase the number of mitochondria aggregates in park (Supplemental Fig. 10), but not Pink1 (not shown), KD flies indicating that park is more ref(2)P/p62-dependent for mitophagy induction. Therefore, in this biological setting cncC/Nrf2-induced mitophagy is likely independent of the autophagy/mitophagy adapter ubiquitin-binding protein ref(2)P/p62.

Thus, cncC/Nrf2 OE activated proteostatic modules and reverted (independently to ref(2)P/p62) the formation of Mito-GFP aggregates in park or Pink1 RNAi expressing flies.

cncC/Nrf2 activation alleviates the neuromuscular degeneration phenotypes of park or Pink1 KD mutant flies

Finally, we asked whether cncC/Nrf2 OE could also rescue the neuromuscular degeneration phenotypes manifested in park or Pink1 KD flies. Gene expression analyses in isolated brains after neuronal-targeted (Gal4Elav) co-expression of the Tgs showed that targeted cncC/Nrf2 OE upregulated proteasomal subunits and mitochondrial genes but not ref(2)P/p62 and Atg8a (Fig. 6A; see also Fig. 3E1), indicating tissue-specific responses after cncC/Nrf2 OE. Importantly, PGC1-a was also induced by cncC/Nrf2 OE (Fig. 6A). Previous reports have shown that apart from a master regulator of mitochondria biogenesis, PGC1-a also exerts a neuroprotective role [31]. Also, cncC/Nrf2 OE in nervous tissues increased proteasome activities (Fig. 6B), reduced oxidative load (Fig. 6C) and as found in muscles, enhanced the overall mitophagy turnover rates in park or Pink1 KD flies’ brain (Fig. 6D). In support, it prevented loss of DA neurons in the brain of park or Pink1 KD flies (Fig. 6E), rescued the park, Pink1 KD mutant’s locomotion defects (Fig. 6F) and tended to improve, especially in the park KD background which showed the more severe phenotype, young flies’ health-span (Supplemental Fig. 11 and Supplemental Table S1). Yet, consistently to our recent findings showing that prolonged cncC/Nrf2 OE in the fly can be toxic [21] the overall longevity of these transgenic flies was not (vs. controls) improved (Supplemental Fig. 11 and Supplemental Table S1).

Fig. 6: OE of cncC/Nrf2 alleviates the neuromuscular degeneration phenotypes induced by nervous tissues-targeted park or Pink1 KD.
figure6

A Relative mRNA expression of proteasomal subunits, cncC/Nrf2, autophagy related (Atg8a, ref(2)P/p62) and mitochondrial genes in dissected brains of the indicated transgenic flies. B, C Relative (%) 26S chymotrypsin-like (CT-L) and caspase‐like (C‐L) proteasome activities (B), and ROS levels (C), (head samples) after cncC/Nrf2 OE in Pink1 or park KD flies. D1 CLSM visualization of the Mito-QC reporter GFP-mCherry signal in brain DA neurons (Gal4TH), and (D2) quantification of brain (Gal4Elav), DA (Gal4TH) mitolysosome number of the shown adult transgenic flies. E1 CLSM images of DA PPL1 neurons (middle-aged flies) following staining of dissected brains with anti-TH antibody, and (E2) quantification of DA PPL1 neurons at the indicated adult transgenic flies’ brain. F Locomotion (climbing) activity of the shown transgenic flies. In (AF) Tgs were expressed specifically in the nervous tissues (Gal4TH and/or Gal4Elav). Gene expression in (A) was plotted vs. control set to 1; RpL32/rp49 gene was used as reference. Bars, ±SD; n = 3, *P < 0.05; **P < 0.01.

Taken together, these data support the notion that activation of the druggable cncC/Nrf2 pathway can improve the severe effects of age-related park or Pink1 reduced functionality.

Discussion

UPP and ALP play a critical role in regulating cellular functionality and viability and the decline of their activity is a hallmark of aging, and of multiple age-related diseases including neuromuscular degenerative disorders [16, 32]. Seemingly, there is an extensive functional wiring between antioxidant, proteostatic, and mitostatic modules [21, 22, 29, 33] and both antioxidant and proteostatic pathways curate mitochondrial functionality by triggering adaptive repair responses, mitochondrial biogenesis, and/or even removal of the damaged organelle by mitophagy; insufficiency of these responses can induce cell death [34].

We report that park or Pink1 KD in young flies modulated proteostatic modules in a tissue-dependent manner; ubiquitously increased cell oxidative load (shown also after loss-off PINK1 function in SH-SY5Y human neuroblastoma cells [35]), and suppressed mitophagy in neuronal and muscle tissues causing mitochondrial aggregation and neuromuscular degeneration. Pink1 exploits the canonical mitochondrial import pathway and under physiological conditions faces proteasome degradation [36], while the E3 ubiquitin ligase Parkin is one of the numerous UPP components recruited at the OMM upon mitochondrial damage [7, 37]. The mitochondrial localization of these components is another indication of the linkage between proteostatic-mitostatic modules; a notion that is further supported by the finding that ~62% of the mitochondrial proteome might be ubiquitinated for UPP degradation [33]. Notably, park KD led to increased proteasomal enzymatic activities, while, Parkin-mediated mitophagy is reduced when proteasome is inhibited and proteasomes accumulate on mitochondria during Parkin-induced mitophagy [38, 39]. Thus, proteasomal activities are tightly regulated as a response to altered functionality of Parkin. On the other hand, it has been reported a deficit in proteasome activity in Pink1 loss-of-function cells and it was suggested that this readout may relate to reduced ATP production that Pink1 loss-of-function cells displayed [40]. Considering that both park and Pink1 KD mutant flies showed similar mitochondrial oxidative phosphorylation capacity, we hypothesize that the differential effects on proteasome activities in park, Pink1 KD flies may also relate to the different ubiquitination profiles that was induced in flies’ tissues after Pink1 or park KD. Indeed, UPP degradation rates depend on proteome ubiquitination and the balance between ubiquitination/de-ubiquitination of the substrates [41].

Distinctly to park KD, downregulation of Pink1 upregulated Atg8a/Gabarap expression and lysosomal cathepsins activity, indicating the activation of autophagic responses; in support PINK1 knockout in mouse embryonic fibroblasts resulted in autophagy upregulation [42]. Yet, in either park or Pink1 KD mutant flies’ tissues there was minimal co-localization between ALP components and mitochondria aggregates, further supporting the notion that mitophagy is regulated distinctly from bulk autophagy [43]. In our study in the fly model, Pink1 KD showed less intense phenotypes as compared to park KD. We hypothesize that this may relate to the fact that park functions downstream of Pink1 [28] and thus, in Pink1 RNAi flies the remaining active protein could partially activate park. Given also the differential readouts in terms of proteostatic responses and levels of tissue oxidative load upon ubiquitous or tissue-targeted park or Pink1 KD, it is likely that mitophagy displays (at least in flies) tissue-dependent regulation. However, we cannot exclude that the noted park or Pink1 genes silencing effects could be partially independent of their role in mitophagy [44, 45].

Among the most challenging questions regarding mitochondrial removal is the role of Parkin/Pink1 in basal mitophagy. Similarly, to previous reports in C. elegans [46] we found high basal mitophagy rates in flies’ tissues, which was nonetheless marked by tissue-dependency as basal mitophagy was higher in neuronal vs. muscle tissues. Previous studies suggested lack of basal mitophagy in Drosophila adults or pupae muscles [30]; yet we found increased rates of basal mitophagy in larvae muscles. This discrepancy likely relates to our observation that detection of mitophagy is affected by larvae developmental stage or tissue preparation. In support, lysosomes are abundant in flies’ muscles [47] and it was suggested that their detection using probes should be made in live tissues [48]. Given the impact of tissue preparation or the developmental stage on mitophagy recording, the molecular mechanisms of this pathway, under basal or stress conditions, should be preferentially studied at in vivo models [46]. To this end, the analysis of transgenic animals expressing mitophagy reporters, such as Mito-QC, will further increase our understanding on mitophagy regulation.

Muscles were particularly sensitive to reduced park or Pink1 expression as we found that KD of Pink1 and especially of park reduced mitophagy events in muscles, a tissue of high metabolic demand. This phenotype explains the excessive accumulation of mitochondrial aggregates in transgenic flies’ muscles along with the muscle related degenerative phenotypes seen in young flies, i.e. disrupted wing posture and locomotion activity. Interestingly, our findings indicate that the role of Pink1 in Drosophila brain is dispensable for basal mitophagy, differently from park which (as in muscles) seemingly plays an important role on driving mitophagy events also in neuronal tissues. In support, the role of Pink1 in basal mitophagy is dispensable in several mouse tissues [49]. Up to date, little is known about the regulation of mitophagy in neurons and the functional involvement of the park/Pink1 pathway in this tissue. Given the high dependence of neurons on proper mitochondria functionality, one would assume that these cells have evolved additional pathways to remove damaged mitochondria [50]. Indeed, several studies have demonstrated the existence of park/Pink1-independent mitophagy cascades revealing multiple mitochondrial proteins (FUNDC1, BNIP3, NIX, and PHB2) or even lipids (e.g., cardiolipin) that mediate mitophagy in response to different stimuli [51,52,53].

Given the impact of mitochondrial dysfunction on neurodegeneration disorders we sought to rescue the Pink1 or park KD-mediated deleterious effects. We found that concomitant to park or Pink1 KD, activation of the cncC/Nrf2 transcription factor [20, 21], ubiquitously induced the proteostasis network, suppressed oxidative stress and restored mitochondrial function and mitophagy rates in flies’ tissues; furthermore, our data indicate that cncC/Nrf2 is a transcriptional regulator of park and Pink1 genes. Reportedly, a subset of the cytoplasmic Nrf2/Keap1 ROS-sensing complexes are tethered to OMM via the interaction of Keap1 with the mitochondrial protein PGAM5 [54], while in C. elegans, Nrf2 coordinates both mitochondrial removal and biogenesis upon oxidative stress [55]. Interestingly, 1,4-diphenyl-1,2,3-triazole (PMI), was found to mediate LC3 recruitment to mitochondria and to increase mitochondrial p62 co-localization in a Nrf2-dependent way [56]. Our data showed that cncC/Nrf2 can mediate mitophagy at the park or Pink1 KD genetic backgrounds in a ref(2)P/p62-independent manner, suggesting that Nrf2 likely engages additional ubiquitin receptors to mediate mitophagy. Indeed, Nrf2 is a transcriptional activator in mammalian cells of the autophagy receptor NDP52 [57]; yet the identification of Nrf2-inducible receptors in Drosophila mitophagy should await further studies.

Finally, we observed that Nrf2 activation in the fly model largely rescued park or Pink1 KD-mediated neuromuscular degenerative phenotypes. Specifically, cncC/Nrf2-targeted activation in neuronal tissues expressing park or Pink1 RNAi, reduced oxidative load, tended to increase mitophagy and rescued loss of DA neurons in the brain. In DA neurons the park/Pink1 pathway is overactive, probably because of the high mitostatic stress that these cells face [58]; also, increased mitophagy rates due to cncC/Nrf2 OE in surrounding to DA neurons cells, could establish a by-stander effect that reduces oxidative load and provides survival signals. Consistently, Nrf2-activating molecules such as sulforaphane or RTA-40 can restore mitochondrial membrane potential and have protective effects in neurodegeneration [59]. Thus, Nrf2 activation can represent a promising therapeutic intervention in degenerative diseases.

Availability of Data and Materials

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Dirk Bohmann (University of Rochester, NY, USA), Alexander Whitworth (University of Cambridge, UK) and Genny Orso (University of Padova) for fly lines. SG and MSM are recipients of a Post-Doc and PhD, respectively, fellowship from the Hellenic State Scholarships Foundation; this research is co-financed by Greece and the European Union (European Social Fund through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014-2020”). I.P.T. acknowledges funding the Hellenic GSRT project BIOIMAGING-GR (MIS 5002755).

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I.P.T. designed and supervised the study; S.G., E.D.P., M.S.M., and I.P.T. conducted experiments or interpreted the data; S.G., E.D.P., and I.P.T. wrote the manuscript.

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Correspondence to Ioannis P. Trougakos.

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Gumeni, S., Papanagnou, ED., Manola, M.S. et al. Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes. Cell Death Dis 12, 671 (2021). https://doi.org/10.1038/s41419-021-03952-w

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