A Immunoblotting of POU2F2 and PDPK1 in GBM cells with or without POU2F2 silence and restoration of POU2F2. B Predicted sequences of POU2F2 motif in PDPK1 promoter by JASPAR. C ChIP-qPCR analysis of POU2F2 levels at different regions of PDPK1 promoter in LN229 cells. D ChIP-qPCR analysis of POU2F2 levels at PDPK1 promoter in LN229 and U-118 cells expressing shCtrl or shPOU2F2. E Luciferase promoter/reporter constructs containing the truncated wild PDPK1 promoter or POU2F2 motif mutant were co-transfected with a shPOU2F2 or with an empty vector (shCtrl) into HEK293FT cells, and luciferase activity was evaluated 24 h later. F Immunoblotting of POU2F2, PDPK1, AKT, p-AKT, mTOR, and p-MTOR in GBM cells with or without POU2F2 silence and restoration of POU2F2. All data were shown as the mean ± SD, **p < 0.01, ***p < 0.001.