Fig. 4: POU2F2 promotes aerobic glycolysis and cell growth by activating AKT/mTOR pathway. | Cell Death & Disease

Fig. 4: POU2F2 promotes aerobic glycolysis and cell growth by activating AKT/mTOR pathway.

From: POU2F2 regulates glycolytic reprogramming and glioblastoma progression via PDPK1-dependent activation of PI3K/AKT/mTOR pathway

Fig. 4

A, B GSEA showed significant enrichment of gene sets involved in the PI3K/AKT/mTOR pathway, with most of the genes being downregulated by POU2F2 depletion in B Cell lymphoma cells. C Immunoblotting of AKT, p-AKT (T308), mTOR, p-MTOR (S2448), GLUT1, HK2, and PKM2 in GBM cells with or without POU2F2 silence. D Glucose uptake, consumption, lactate production of LN229 cells with or without POU2F2 induction and XIV (20 μM). E Glycolytic flux changes of LN229 cells with or without POU2F2 induction and XIV. F Growth assays of LN229 cells with or without POU2F2 induction and XIV. G Immunoblotting of AKT, p-AKT, mTOR, p-MTOR, GLUT1, HK2, and PKM2 in LN229 cells with or without POU2F2 induction and XIV, β-actin levels were shown as loading control. All data were shown as the mean ± SD, *p < 0.05, **p < 0.01.

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