A Western blot analyses of POU2F2 expressions in LN229 and U-118 cells transduced with POU2F2 shRNA clones or shRNA scramble. B The effect of POU2F2 on the proliferation of GBM cells was detected by MTT assays. C Image and quantification of GBM cells positive for BrdU staining. D The cell cycle analyses were performed in cells with shCtrl and shPOU2F2. The percentage of cells in the different phases were indicated. E, F Immunoblotting of POU2F2, CCND1, CCNE2, CDK2, CDK4, and CDK6 in GBM cells with or without POU2F2 silence. All data were shown as the mean ± SD, **p < 0.01.