A Immunohistochemical staining analysis of POU2F2 in normal brain tissues and 12 paired GBM tissue samples. B Immunohistochemistry analyses of POU2F2 expression levels in eight normal brain samples and 12 GBM samples. C Kaplan–Meier analysis of overall survival from the R2 database with the log rank test P value indicated. D Quantitative RT-PCR analysis of POU2F2 mRNA levels in 13 paired GBM and peritumoral normal tissues. E, F Quantitative RT-PCR and western blot analysis of POU2F2 expression in normal astrocytes (SVGP12) and GBM cell lines. All data were shown as the mean ± SD, *p < 0.05, **p < 0.01. All p values were based on analysis control versus treatment.