Fig. 3: Ectopic expression of upd2 enhances, while loss of upd2 suppresses mahe induced apoptosis in developing eye. | Cell Death & Disease

Fig. 3: Ectopic expression of upd2 enhances, while loss of upd2 suppresses mahe induced apoptosis in developing eye.

From: Maheshvara regulates JAK/STAT signaling by interacting and stabilizing hopscotch transcripts which leads to apoptosis in Drosophila melanogaster

Fig. 3

ae Images of Drosophila adult eye. a′–e Eye imprints of adult eye. GMR-GAL4 was used to drive the expression of UAS-mahe (a, c, e) or UAS-upd2 (b, c). a, a GMR-GAL4 > mahe promotes eye roughening. b, b GMR-GAL4 driven upd2 resulted in severely overgrown eyes with regular patterned ommatidia. c, c Coexpression of upd2 and mahe leads to pupal lethality whereas few escapers showed drastic eye roughness, with loss of pigmentation, black necrotic patches and loss of ommatidia in comparison to ectopic expression of mahe alone. d, d upd2∆3-62 a loss-of-function allele shows slightly disorganized and fused ommatidia along the dorsal side of adult eye. e, e upd2∆3-62 in combination with GMR-mahe suppressed the disorganized adult eye phenotype. fj and ko Acridine orange and caspase staining (compare area marked within rectangle) in eye-antennal disc of third instar larvae. f, k Acridine orange and caspase staining marked dying cells on ectopic expression of mahe. g, l Fewer acridine orange and caspase positive cells were observed upon upd2 overexpression. h, m Coexpression of upd2 and mahe enhanced cell death associated with mahe, which is reflected by increase in acridine orange and caspase positive cells when compared to mahe alone. i, n upd2∆3-62 showed fewer acridine and caspase positive cells. j, o upd2∆3-62 rescued the mahe induced cell death, which is depicted by reduced number of acridine orange and caspase positive cells. p Graph represents cl-caspase intensity per unit area which shows upd2 overexpression promotes mahe induced cell death, and was significantly reduced in combination with upd2 mutant. One-way ANOVA. Scale bar in 50 µm (ae, fj, ko). Genotypes (a) w/+; +; GMR-GAL4,UAS-HA-mahe/+ (b) w/+; UAS-upd2:GFP/+;GMR-GAL4/+ (c) w/+;UAS-upd2:GFP/+;GMR-GAL4,UAS-HA-mahe/+ (d) upd2∆3.62/+; +;+ (e) upd2∆3.62/+; +; GMR-GAL4, UAS-HA-mahe. One-way ANOVA * P < 0.05, ** P < 0.01, *** P < 0.001.

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