Fig. 2: Ectopic expression of mahe promotes caspase-dependent dosage sensitive cell death in eye tissue of Drosophila. | Cell Death & Disease

Fig. 2: Ectopic expression of mahe promotes caspase-dependent dosage sensitive cell death in eye tissue of Drosophila.

From: Maheshvara regulates JAK/STAT signaling by interacting and stabilizing hopscotch transcripts which leads to apoptosis in Drosophila melanogaster

Fig. 2

ae Images of Drosophila adult eyes. a′e′ Eye imprints of adult eye. GMR-GAL4 was used as a control (a), or to drive expression of mahe (be), P35 (d), DIAP1 (e). a, a′ Control adult eye showed normal ommatidium arrangement. b, b GMR-GAL4 > mahe induced eye roughness with fused ommatidia (marked with arrow). c, c Ommatidial disarray and fusion were severely enhanced on increasing the dosage of mahe. d, d mahe induced eye phenotype was suppressed by expression of P35, an inhibitor of effector caspase. e, e Similar to P35, inhibitor of initiator caspase DIAP1 suppressed the eye roughening and ommatidium disarrangement. fj Acridine orange staining of eye-antennal imaginal discs. f Acridine orange marks few dying cells in control eye-antennal disc. g, h Number of acridine orange positive cells were enhanced upon mahe overexpression in dosage sensitive manner. i, j P35 and DIAP1 suppressed cell death, shown by reduction in acridine positive nuclei. k–o Caspase immunostaining of eye-antennal discs from third instar larvae (Compare area within rectangle). l, m Ectopic mahe leads to increase in number of caspase positive cells in comparison to wild type (k). n, o Reduction in caspase positive cells were observed upon coexpression of P35 and DIAP1 along with mahe. (p) Graph represents intensity of cl-casp3 per unit area showing mahe triggers caspase-dependent cell death, which was suppressed by P35 and DIAP1. One-way ANOVA. Scale bar in 50μm (ae, fj, ko). Genotypes (a) w/+; +; GMR-GAL4/+ (b) w/+; +; GMR-GAL4, UAS-HA-mahe/+ (c) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-HA-mahe (d) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-P35 (e) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-DIAP1. One-way ANOVA * P < 0.05, ** P < 0.01, *** P < 0.001.

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