a–e Images of Drosophila adult eyes. a′–e′ Eye imprints of adult eye. GMR-GAL4 was used as a control (a), or to drive expression of mahe (b–e), P35 (d), DIAP1 (e). a, a′ Control adult eye showed normal ommatidium arrangement. b, b′ GMR-GAL4 > mahe induced eye roughness with fused ommatidia (marked with arrow). c, c′ Ommatidial disarray and fusion were severely enhanced on increasing the dosage of mahe. d, d′ mahe induced eye phenotype was suppressed by expression of P35, an inhibitor of effector caspase. e, e′ Similar to P35, inhibitor of initiator caspase DIAP1 suppressed the eye roughening and ommatidium disarrangement. f–j Acridine orange staining of eye-antennal imaginal discs. f Acridine orange marks few dying cells in control eye-antennal disc. g, h Number of acridine orange positive cells were enhanced upon mahe overexpression in dosage sensitive manner. i, j P35 and DIAP1 suppressed cell death, shown by reduction in acridine positive nuclei. k–o Caspase immunostaining of eye-antennal discs from third instar larvae (Compare area within rectangle). l, m Ectopic mahe leads to increase in number of caspase positive cells in comparison to wild type (k). n, o Reduction in caspase positive cells were observed upon coexpression of P35 and DIAP1 along with mahe. (p) Graph represents intensity of cl-casp3 per unit area showing mahe triggers caspase-dependent cell death, which was suppressed by P35 and DIAP1. One-way ANOVA. Scale bar in 50μm (a′–e′, f–j, k–o). Genotypes (a) w/+; +; GMR-GAL4/+ (b) w/+; +; GMR-GAL4, UAS-HA-mahe/+ (c) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-HA-mahe (d) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-P35 (e) w/+; +; GMR-GAL4,UAS-HA-mahe/UAS-DIAP1. One-way ANOVA * P < 0.05, ** P < 0.01, *** P < 0.001.