Transcriptome analysis was done of eye tissue overexpressing mahe driven with GMR-GAL4 and was compared to GMR-GAL4 controls. a, b Functional categories enriched in upregulated (1080) and downregulated (519) genes based on their molecular functions using DAVID, which includes genes associated with phototransduction, JAK/STAT pathway activation, response to stress, nuclear division, and many more. c, d Enrichment of pathway specific upregulated (1080) and downregulated (519) genes based on their biological and cellular functions using DAVID. e Graph represents selected genes upd1, upd2, upd3 and socs36E were differentially expressed in the RNA-Seq data. f Real time PCR validated the candidate genes obtained from our transcriptome analysis data. The components of JAK/STAT signaling pathway were upregulated upon ectopic expression of mahe and were significantly downregulated in mahe loss-of-function mutant as well as in mahe-RNAi. One-way ANOVA. Genotypes (a–f) w/+; +; GMR-GAL4/+ and w/+; +; GMR-GAL4, UAS-HA-mahe/UAS-HA-mahe (f) EP∆mahe d08059;+;+ (f) +; UAS-mahe-RNAi/+; GMR-GAL4/+. One-way ANOVA *P < 0.05, **P < 0.01, ***P < 0.001.