Fig. 5: HOXC6 activates Wnt/β-catenin via inhibiting DKK1 excretion. | Cell Death & Disease

Fig. 5: HOXC6 activates Wnt/β-catenin via inhibiting DKK1 excretion.

From: HomeoboxC6 promotes metastasis by orchestrating the DKK1/Wnt/β-catenin axis in right-sided colon cancer

Fig. 5

A Co-IP using Flag-gel was performed with HCT116 cells in the HOXC6-OE and NC groups. Whole-cell lysate was loaded as a positive control. β-catenin, DKK1, and HOXC6 were evaluated in the Flag-HOXC6 complex. B HOXC6 and DKK1 fluorescent colocalization in 293 T cells. C HOXC6 and DKK1 fluorescent colocalization in HCT116 cells. D Dual luciferase assay testing the ability of HOXC6 to bind to the DKK1 promoter with WT and mutant sequences in HEK 293T cells. E ELISA detection of DKK1 concentration in cell supernatants in different HCT116 experimental groups. F Schematic diagram for the potential binding sequence of HOXC6in the DKK1 promoter. G Transwell experiment in the HOXC6-OE siCtrl, HOXC6-OE siβ-catenin, and HOXC6-OE siDKK1 groups in HCT116 cells. H Numbers of migration HCT116 cells in the HOXC6-OE siCtrl, HOXC6-OE siβ-catenin, and HOXC6-OE siDKK1 groups. I Expression of β-catenin, HOXC6, DKK1, ZO1, E-cadherin, Vimentin, and snail in HOXC6-OE siCtrl, HOXC6-OE siβ-catenin, and HOXC6-OE siDKK1 groups in HCT116 cells. ***: P < 0.001.

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