A Pairwise correlation between Axin2 and HOXC6 using TCGA expression profiles. B Pairwise correlation between RNF43 and HOXC6 using TCGA expression profiles. C Expression of β-catenin, c-Jun, RNF43, Axin2, DKK1, and HOXC6 was evaluated by WB in whole-cell lysates of HOXC6-OE, HOXC6-KD, and their control groups in the HCT116 cell line. D The expression of β-catenin, c-Jun, and HOXC6 was evaluated in the cytoplasm and nucleus of HCT116 cells in the HOXC6-OE, HOXC6-KD, and control groups. Tubulin was used as a cell plasma protein and whole-cell protein loading control. Histone-H3 was used as a nuclear protein loading control. E GSEA showed that the EMT process was significantly enriched in the HOXC6-overexpressing group using TCGA dataset. F Protein expression of ZO-1, E-cadherin, vimentin, snail, and HOXC6 in three different treated HCT116 groups. G Relative mRNA expression of β-catenin, DKK1, and HOXC6 in the NC, HOXC6-KD, and HOXC6-OE groups in HCT116 cells. *: P < 0.05; ***: P < 0.001.