Fig. 3: Oleandrin induces ICD-associated immune activation in vitro. | Cell Death & Disease

Fig. 3: Oleandrin induces ICD-associated immune activation in vitro.

From: Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer

Fig. 3

DCs were co-cultured with MDA-MB-231 with or without oleandrin treatment for 48 h. DCs were sorted by staining with CD45. A The relative expression of CD80, CD86, IL-10, IL-2, and IFNG in DCs were detected by qRT-PCR. B The expression of CD80, CD86 and HLA-DR on DCs surface were further detected by flow cytometry. Cells stained with isotype antibody were used as control. Data were represented by ΔMFI values. C MDA-MB-231 cells were pretreated with oleandrin for 24 h and co-cultured with DCs for further 48 h. Cytokine secretion in the culture supernatant was detected by ELISA. D The cell growth of the following groups was determined by clone-formation assay: MDA-MB-231, MDA-MB-231/DCs, MDA-MB-231/CD8+ T cells, MDA-MB-231/DCs/CD8+ T cells, 25 nM oleandrin-treated MDA-MB-231, and 25 nM oleandrin-treated MDA-MB-231/DCs/CD8+ T cells. The representative pictures (upper) and quantification data (lower) were shown. Data are presented as mean ± standard error of the mean from three independent experiments. **p < 0.01. 231, MDA-MB-231; ole, oleandrin.

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