Fig. 1: Neurog2 converts dorsal midbrain astrocytes into neurons in vivo. | Cell Death & Disease

Fig. 1: Neurog2 converts dorsal midbrain astrocytes into neurons in vivo.

From: Neurog2 directly converts astrocytes into functional neurons in midbrain and spinal cord

Fig. 1

a–b″″ Double staining of mCherry and NeuN on sections of the dorsal midbrain from WT mice that were infected with the control virus AAV–mCherry (aa″″) or AAV-Neurog2/mCherry (bb″″) on day 3. cd″″ Double staining of mCherry and NeuN on sections of the dorsal midbrain from WT mice that were infected with the control virus AAV–mCherry (cc″″) or AAV-Neurog2/mCherry (dd″″) on day 10. ef″″ Double staining of mCherry and NeuN on sections of the dorsal midbrain from WT mice that were infected with the control virus AAV–mCherry (ee″″) or AAV-Neurog2/mCherry (ff″″) on day 30. Panels (a″″, b″″, c″″, d″″, e″″, f″″) are higher magnifications of the boxed areas in (a″′, b″′, c″′, d″′, e″′, f″′), respectively. mCherry was not colocalized with NeuN (arrowheads). mCherry colocalized with NeuN (arrows). g The statistical data of astrocyte-to-neuron conversion efficiency induced by Neurog2 at different time points. A one-way ANOVA revealed a significant effect of group (F[5,17] = 493.2, p < 0.001), followed by Tukey’s multiple comparison test. *** Represents p < 0.001; scale bars: 50 μm (a″′, b″′, c″′, d″′, e″′, f″′) and 25 μm (a″″, b″″, c″″, d″″, e″″, f″″). hh″ 3D images of (f″″): (h) front view of (f″″), (h′) right-side view of (h) rotated 30°, (h″) bottom view of (h) rotated 30°. Red arrows in (f″″, h, h′, h″) show a typical view of the same cell.

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