A, B Western blot analysis of the protein levels of BCAT1, mTOR, p70S6K, 4E-BP1 and their phosphorylated counterparts and LC3-I/II in Hela cells treated with cisplatin (20 μM) or in combination with BCAAs (5 mM) or Leu (2 mM) for 24 h. C, D Hela cells were treated with increasing concentrations of cisplatin alone or in combination with BCAAs (5 mM) or Leu (2 mM) for 24 h. Cell viability was assessed by CCK-8 assays. E Representative image of Hela cells transfected with pCMV-mCherry-GFP-LC3B. The cells were treated with cisplatin (20 μM) or Leu (2 mM) alone or together for 24 h. Scale bars: 25 μm. Three independent experiments were performed. *P < 0.05, **P < 0.01, ***P < 0.001.