Sequential targeting of YAP1 and p21 enhances the elimination of senescent cells induced by the BET inhibitor JQ1

Zhang, Huan-Tian; Gui, Tao; Liu, Ri-Xu; Tong, Kui-Leung; Wu, Chong-Jie; Li, Zhenyan; Huang, Xun; Xu, Qiu-Tong; Yang, Jie; Tang, Wang; Sang, Yuan; Liu, Wanting; Liu, Ning; Ross, Ryan D.; He, Qing-Yu; Zha, Zhen-Gang

doi:10.1038/s41419-021-03416-1

Download PDF

Article
Open access
Published: 25 January 2021

Sequential targeting of YAP1 and p21 enhances the elimination of senescent cells induced by the BET inhibitor JQ1

Huan-Tian Zhang^1,2,3^na1,
Tao Gui^1,2^na1,
Ri-Xu Liu^1,2,
Kui-Leung Tong²,
Chong-Jie Wu^1,2,
Zhenyan Li^1,2,
Xun Huang⁴,
Qiu-Tong Xu^1,2,
Jie Yang^1,2,
Wang Tang^1,2,
Yuan Sang⁵,
Wanting Liu³,
Ning Liu^1,2,
Ryan D. Ross⁶,
Qing-Yu He ORCID: orcid.org/0000-0003-0503-9492³ &
…
Zhen-Gang Zha ORCID: orcid.org/0000-0003-3394-5871^1,2

Cell Death & Disease volume 12, Article number: 121 (2021) Cite this article

4338 Accesses
11 Citations
1 Altmetric
Metrics details

Subjects

Abstract

Chondrosarcoma (CHS) is the second most common bone malignancy with limited therapeutic approaches. Our previous study has found that Yes associated protein 1 (YAP1) is downregulated in CHS cells treated with bromodomain and extraterminal domain (BET) inhibitor JQ1. However, the precise role of YAP1 in CHS is largely unknown. Herein, we found that YAP1 expression was upregulated in CHS tissues, and positively correlated with its grading score. Loss of YAP1 inhibited CHS proliferation and induced cellular senescence, while expression of YAP1 mutants revealed YAP1/TEA domain family member (TEAD)-dependent negative regulation of p21 and subsequent cellular senescence. These results were validated by in vivo experiments using stable shYAP1 cell lines. Mechanistically, negative regulation of p21 by YAP1 occurred post-transcriptionally via Dicer-regulated miRNA networks, specifically, the miR-17 family. Furthermore, we demonstrated that sequential targeting of YAP1 and p21 enhanced the elimination of JQ1-induced senescent cells in a Bcl-2-like 1 (Bcl-XL)/Caspase-3 dependent manner. Altogether, we unveil a novel role of YAP1 signaling in mediating CHS cell senescence and propose a one-two punch approach that sequentially targets the YAP1/p21 axis to eliminate senescent cells.

Selective inhibition of mutant IDH1 by DS-1001b ameliorates aberrant histone modifications and impairs tumor activity in chondrosarcoma

Article Open access 12 August 2019

Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis

Article Open access 25 July 2022

The crucial p53-dependent oncogenic role of JAB1 in osteosarcoma in vivo

Article 10 May 2020

Introduction

Chondrosarcoma (CHS) is the second most common primary bone malignancy and is characterized by the synthesis of hyaline cartilage. Poor vascularization, slow division rate, and substantial hyaline cartilage production confer resistant to conventional chemotherapy and radiotherapy¹. Over the past 3 decades, complete surgical resection with wide margins remains the major treatment option. Therefore, there is an urgent need to understand the molecular mechanisms that lead to chemo- and radioresistance to develop efficient therapeutic strategies. Several ongoing phases I/II clinical trials have been conducted for CHS, including agents targeting isocitrate dehydrogenase (IDH) mutations, the PI3K-Akt-mTOR, hedgehog, and angiogenesis signaling². In addition, previous studies have highlighted the importance of anti-apoptotic proteins, such as B-cell leukemia/lymphoma 2 (Bcl-2) and Bcl-2-like 1 (Bcl-XL), in mediating cell survival upon chemotherapy and radiotherapy^3,4. Recently, aberrant expression of Yes-associated protein 1 (YAP1) has been observed in a panel of sarcomas including CHS^5,6,7, yet the precise role of YAP1 in CHS remains to be explored.

YAP1 is a mechanosensitive transcriptional regulator that is downstream of the Hippo pathway, which, controls development and tumorigenesis through regulating cell proliferation, survival, differentiation, and cytoskeletal remodeling⁸. The shuttling of YAP1 between the cytoplasm and nucleus is achieved in a phosphorylation-dependent manner (S61, S109, S127, S164, and S381)⁹. Nuclear YAP1 has been shown to interact with different transcription factors, particularly TEA domain family members (TEAD), thereby activating many genes related to cell proliferation and anti-apoptosis effects¹⁰. Accumulated evidences suggest that nuclear YAP1 plays a crucial role in inhibiting cell senescence in fibroblasts or cancer cells^11,12, yet others have demonstrated that hyperactivation of YAP1 can induce cell senescence¹³. The diverse roles of YAP1 in cancers can be attributed to a wide range of regulatory miRNA networks¹⁴; however, whether YAP1 plays a role in regulating cellular senescence via miRNAs is largely unknown.

Cellular senescence is defined as irreversible growth arrest and the production of a bioactive secretome, which has been recognized as a tumor-suppressive mechanism¹⁵. Based on this, the induction of senescence in cancer cells is an emerging therapeutic option. However, recent studies have suggested that the accumulation of senescent cells in tissues and organs exerts detrimental effects, e.g., by driving secondary tumors or triggering cancer relapse^16,17. Therefore, eliminating the senescent cells in a precise time window enables the maximization of therapeutic efficacy. Two canonical pathways, the p53/p21 or the pRb/p16 axes, have been implicated in cellular senescence induced by various insults, including telomere shortening, oncogenic signaling, and therapeutic or stress-induced DNA^18,19. Under therapeutic pressure, p21 expression is greatly induced, which controls cancer cell senescence, however, the substantial expression of p21 can also be detrimental to cancer recurrence²⁰. For instance, persistent expression of p21 causes genomic instability in a p53-independent manner²¹. Moreover, p21 expression favors the survival of senescent cells via c-Jun N-terminal kinase (JNK) and caspase signaling²². These studies indicate that restricting p21 expression might provide an option for precisely eliminating senescent cells.

In the present study, we systematically evaluated the oncogenic role of YAP1 in CHS both in vitro and in vivo and demonstrated that YAP1 was essential for the modulation of CHS cell senescence by targeting the miR-93/p21 axis. Furthermore, we uncovered that the YAP1/p21 axis was implicated in chemotherapy-induced senescence and proposed sequentially targeting YAP1 and p21 as a “one-two punch” approach to maximize therapeutic efficacy by enhancing the elimination of senescent cells.

Results

Nuclear localization of YAP1 confers oncogenic phenotypes in CHS cells

YAP1, a critical downstream effector of the Hippo pathway, has been reported to be involved in the development and progression of several human cancers including sarcoma²³, yet its roles in CHS are largely unknown. Regardless of anatomical location, all CHS are classified by histology into three grades ranging from well-differentiated grade I to poorly differentiated grade III, of which 70% of cases develop metastasis²⁴. This study firstly evaluated the expression of YAP1 in a series of CHS tissues. Notably, the expression of YAP1 was significantly increased with tumor grade from well to poor (Fig. 1A, B). We also observed a predominant localization of YAP1 in the nucleus in CHS tissues (Fig. 1C), which was in agreement with previous findings that YAP1 localizes in the nucleus, thereby initiating oncogenic signalings^25,26. Interestingly, the percentage of nuclear YAP1 was higher in the CHS cells (SW 1353 and Hs 819.T) than that in the chondrocytic cell line, CHON-001 (Fig. 1D, E and Fig. S1a). Overexpression of constitutively activated YAP1 (5SA, located in the nucleus) using lentivirus led to a slender cell morphology change in the CHON-001 cells (Fig. S1b). Moreover, 5SA overexpression resulted in a remarkable suppression of F-actin networks, which are critical factors in the determination of cell stiffness, in human primary chondrocytes in a cell density-independent manner (Fig. S1c). In addition, single-cell AFM analysis demonstrated that elastic modulus and cell adhesion, which are hallmarks of oncogenic transformation²⁷, were significantly attenuated by enforced YAP1 5SA expression (Fig. 1F and Fig. S1d).

**Fig. 1: Nuclear YAP1 is required for the growth of CHS cells.**

In contrast, transient knockdown of YAP1 by the siRNAs (siY#1, siY#2, and siY#4) significantly abolished the growth of SW 1353 cells (Fig. S1e). Consistently, knockdown of YAP1 using shRNAs (i.e., shYAP1#3 and #4) led to significant inhibition of the SW 1353 cell growth and colony formation, despite a compensatory increase in expression of its heterodimer, TAZ (Fig. 1G, H and Fig. S1f). Interestingly, YAP1/TAZ double knockdown did not show the synergistic effect on suppressing cell growth, indicating that YAP1 was the major effector in the SW 1353 cells (Fig. S1g). Similarly, inhibition of colony formation by YAP1 knockdown was observed in another CHS cell line, Hs 819.T (Fig. S1h). Altogether, these data reveal that nuclear YAP1 is required for the acquisition of oncogenic phenotypes and the growth of CHS cells.

YAP1 and TEAD control cell cycle exit and induce cellular senescence

The control of cancer cell growth by YAP1 can be mediated by modulation of the cell cycle, proliferation, and apoptosis²⁸. We thus further explored the roles of YAP1 in the CHS cells. Two validated shYAP1 (i.e., #3 and #4) but not the scramble control (SC) were found to trigger G0/G1 phase cell cycle arrest, as determined by the flow cytometry (Fig. 2A and Fig. S2a). Since YAP1 has been reported to regulate senescence in several cell types^12,29, we then examined whether YAP1 was required for regulating CHS cell senescence. As expected, YAP1 knockdown significantly increased the percentage of senescence-associated β-galactosidase (SA-β-gal)-positive cells in the two different CHS cell strains, including SW 1353 and Hs 819.T (Fig. 2B–D).

**Fig. 2: YAP1/TEAD controls cell cycle exit and induces cellular senescence.**

Since chondrocytes tend to undergo senescence when cultured in vitro, we further explored how YAP1 was involved in chondrocyte senescence. AFM confirmed that knockdown of YAP1 increased the chondrocyte’s elastic modulus, a characteristic of senescent cells, at the single-cell level (Fig. S2b). In addition, we demonstrated that YAP1 was essential for CHS cell proliferation, as evidenced by that loss of YAP1 reduced the percentage of EdU-incorporated cells, along with the suppression of c-Myc (Fig. 2E, F and Fig. S2c–e). Although YAP1 plays a crucial role in controlling cell apoptosis^9,30,31, we found that knockdown of YAP1 dictated CHS underwent senescence rather than apoptosis (Fig. S2f), elucidating a cell-type dependent regulation of YAP1 on CHS.

It has been documented that p21 is one of the primary mediators of cellular senescence³²; we then examined whether there is any alteration of p21 expression. Notably, loss of YAP1 dramatically upregulated the expression of p21 in the SW 1353 cells, regardless of cell density (Fig. 2G and Fig. S2g). We also found that p16, another primary marker of cellular senescence¹⁹, was not involved in shYAP1-induced cellular senescence as YAP1 depletion had few effects on the p16 expression (Fig. S2h). Nuclear localization of YAP1 and subsequent interaction with TEAD are critical for initiating downstream signaling of the Hippo/YAP1 axis^26,33, we therefore asked whether the interaction of YAP1 with TEAD played a role in CHS cell senescence. To this end, we transiently introduced two YAP1 mutants (i.e., HA-YAP1-5SA and HA-YAP1-5SA/S94A) into cells stably expressing SC or shYAP1#4 for further functional study. As expected, overexpression of HA-YAP1-5SA upregulated the expression of its target gene Cyr61 and, reduced p21 expression and p21-induced cellular senescence in cells expressing shYAP1#4 but not SC (Fig. 2H–J). In contrast, the transcriptionally inactive variant HA-YAP1-5SA/S9A had a marginal effect on p21 expression and p21-induced cellular senescence (Fig. 2H–J). Similar results were obtained in primary chondrocytes using these mutants (Fig. S2i–k). Taken together, these results suggest that YAP1 depletion inhibits cell proliferation and induces cellular senescence, and the latter is achieved by the modulation of p21 expression involving the YAP1-TEAD interaction.

Growth inhibition by YAP1 depletion is negatively correlated with p21 expression in vivo

Next, we evaluated the role of YAP1 in xenograft tumor growth in vivo. SW 1353 cells stably expressing SC, shYAP1#3 or shYA1P#4 were inoculated into the flanks of nude mice to establish the xenograft tumors. Loss of YAP1 in the shYAP1#3 and shYAP1#4 groups significantly inhibited the growth of stable cell-derived xenograft tumors (Fig. 3A). At the time of sacrifice, tumor size was measured via both ex vivo and in vivo. The tumor size in mice injected with shYAP1#3- and shYAP1#4-expressing SW 1353 cells was smaller than that in mice that received SC-expressing SW 1353 cells (Fig. 3B, C). In addition, histological assays showed that tumors in mice received shYAP1#3- and shYAP1#4-expressing SW1353 cells exhibited an inhibitory effect on the mitotic index (Fig. 3D, E). Furthermore, loss of YAP1 resulted in an obvious reduction of Ki67-expressing cells (Fig. 3F, G), which is the proliferative marker of multiple cancers. Since a causal relationship between YAP1 and p21 was observed at the cellular level aforementioned, we then investigated the correlation of YAP1 with p21 in this in vivo model. As expected, an increase in p21-positive cells was observed upon YAP1 depletion by shYAP1#4 and shYAP1#3 (Fig. 3F, G). In addition, the suppressive role of YAP1 in CHS tumor growth was further evaluated by subcutaneously inoculating the SC and shYAP1#4 stable cells into the nude mice at the right flank next to the forelimb. As shown in Fig. 3H–K, knockdown of YAP1 did remarkably inhibit the xenograft tumor growth, accompanied by the reduction of c-Myc-stained positive cells and the induction of senescent cells as determined by the SA-β-gal staining using frozen sections. These results together demonstrate that YAP1, via control of p21 expression, is required for xenograft tumor growth in vivo.

**Fig. 3: YAP1 coordinates with p21 to control the growth of xenograft tumors.**

p21 expression is required for the survival of YAP1 depletion-induced senescent cells

It has been demonstrated that p21 plays a role in regulating cellular senescence owing to its activity as an inhibitor of cyclin-dependent kinase 2 (CDK2) and CDK4³⁴, we therefore examined whether p21 is required for CHS cell senescence using Verteporfin (VP), a small chemical that can inactivate the YAP1 pathway³⁵. A lower concentration of VP significantly upregulated p21 expression and increased the percentage of senescent cells (Fig. 4A, B and Fig. S3a), while a higher concentration of VP suppressed the p21 expression, accompanied by pronounced cell apoptosis (Fig. 4C, D).

**Fig. 4: p21 expression maintains the viability of YAP1 depletion-induced senescent cells.**

Recent studies have suggested that persistent expression of p21 may function as an oncogene by promoting the survival of senescent cells²², therefore we next investigated whether fine-tuning of p21 expression maintains the viability of senescent cells. Indeed, knockdown of p21 by sip21#3 and sip21#4 remarkably abolished the cell senescence induced by the shYAP1#3 or shYAP1#4 (Fig. 4E, F). We also found a marked increase in DNA damage upon p21 knockdown, as seen from the representative images of the DNA tails and the calculation of the olive tail moment (OTM) (Fig. 4G, H). In addition, we also noted that knockdown of p21 increased γ-H2AX expression, accompanied by the activation of caspase-3-dependent apoptosis (Fig. 4I, J). To further implicate the functional link of YAP1/p21 in CHS, the xenograft tumors established by the SC and shYAP1#4 stable cells were subsequently treated with a p21 inhibitor, UC2288. As shown in Fig. 4K, although non-statistical significance was found upon p21 inhibition (intraperitoneal injection of UC2288) in the shYAP1#4 group, we did found that p21 inhibition visibly decreased the percentage of senescent cells (Fig. 4L). Accordingly, TUNEL staining also revealed that p21 inhibition increased cell apoptosis in the xenograft tumors established by shYAP1#4 (Fig. 4M, N). These findings suggest that the downregulation of p21 expression may switch cellular senescence to apoptosis via the induction of DNA damage in YAP1-depleted cells (Fig. 4O).

miR-93 is crucial for YAP1-regulated p21 expression and cellular senescence

In response to cellular stimuli, p21 expression is tightly regulated at the transcriptional and posttranslational levels through mechanisms involving RNA stabilization, phosphorylation, and ubiquitination³⁶. We thus wondered to elucidate the mechanism(s) underlying YAP1 regulation of p21. In agreement with previous reports, mRNA expression of three YAP1 downstream target genes (i.e., ANK, Cry61, and CTGF) was significantly suppressed upon YAP1 depletion. In contrast, a slight increase in p21/CDKNA1 mRNA was noted in one of the shRNAs targets of YAP1 (Fig. S3b). p53 is a master regulator of p21 during cellular senescence^37,38,39. We then tested whether p21 induction was mediated by the p53, although shYAP1#4 did not induce p53 activation (Fig. S3c). Loss of p53 expression induced by its siRNAs (i.e., sip53#2 and sip53#4) did not abolish the induction of p21 by YAP1 depletion, although p53 knockdown partially attenuated the basal expression of p21 (Fig. S3d). These results indicate that the p21 induction does not occur at p53-dependent transcriptional level.

According to the previous study, Sp1 is an alternative way to positively regulate p21 expression⁴⁰, we next explored the possible transcriptional regulation of p21 by Sp1. Our results herein showed that the expression of Sp1 was downregulated upon YAP1 depletion, excluding the possibility that p21 may be positively regulated by Sp1 (Fig. S3e). Notably, substantial induction of p21 in the cytoplasm was observed upon YAP1 depletion (Fig. S3e), suggesting that the regulation of p21 by YAP1 may occur at the protein level. To confirm this, we used a proteasome inhibitor, MG132. MG132 treatment resulted in the accumulation of p21 in control shRNA; however, it did not further enhance p21 induction in YAP1-depleted cells (Fig. S3f).

The Hippo-YAP1 signaling pathway is critical for the biogenesis and maturation of numerous miRNAs through modulating key enzymes such as Dicer^41,42. We then hypothesized that YAP1 regulates p21 expression via the Dicer-miRNA network. As expected, loss of YAP1 function attenuated Dicer expression in the SW 1353 cells (Fig. 5A). Next, we aimed to determine the miRNA profiles that were regulated by the YAP1 using high-throughput small RNA sequencing. A total of 154 miRNAs were up- and downregulated upon YAP1 depletion (Fig. 5B and Fig. S4a). By overlapping the 39 predicted miRNAs that target p21 with the sequencing data, 11 miRNAs were highly regulated by YAP1 in SW 1353 cells (Fig. 5C), and 6 (miR-17, miR-20a, miR-20b, miR-93, miR-106a, miR-106b) out of these 11 belonged to the miR-17 family (Fig. 5D, E). Next, suppression of the expression of miR-17, miR-20a, miR-20b, miR-93, miR-106a, and miR-106b by YAP1 depletion was confirmed by quantitative real-time PCR (Fig. 5F). These findings were supported by a previous study showing that knockout of Dicer also induced robust suppression of miR-17 family members⁴³, with the maximal suppressive effect on miR-93 (Fig. S4B).

**Fig. 5: Regulation of p21 by miR-93 is essential for YAP1 depletion-induced cellular senescence.**

Furthermore, we explored the possible role of the miR-17 family in YAP1-regulated p21 expression and cellular senescence by taking miR-93 as an example. Strikingly, transfection of the miR-93 mimic further inhibited the expression of p21 (Fig. 5G and Fig. S4c), accompanied by the attenuation of cellular senescence induced by YAP1 depletion (Fig. 5H, I). Interestingly, expression of p21 was increased following transfection with the miR-93 inhibitor in the SC cells, while a comparable expression of p21 was found in cells expressing shYAP#3 and shYAP#4 (Fig. 5J and Fig. S4c), indicating the possible saturation of inhibitory effects of miR-93 on the p21 expression. A corresponding change in the cellular senescence upon miR-93 inhibition was also observed (Fig. S4d, e). Although we cannot exclude the possibility that p21 protein stability might be regulated through other mechanisms, these data collectively suggest that the Dicer1/miR-93 axis plays an important role in regulating p21 expression and subsequent cellular senescence upon YAP1 depletion.

Sequential targeting of the YAP/p21 axis promotes the elimination of senescent CHS cells

Having established a regulatory role of YAP1 in p21 expression, we wondered to investigate whether the YAP1/p21 axis is implicated in chemotherapy, given that many first-line chemotherapeutic agents trigger cancer cells to undergo senescence^44,45. As expected, YAP1 expression was downregulated by the first-line chemotherapeutic drugs cisplatin and etoposide, concomitant with the induction of p21 expression in chemotherapy-resistant SW 1353 cells and chemotherapy-sensitive U2 OS cells, in which we had found that knockdown of YAP1 inhibited cell growth and dictated cellular senescence (Fig. 6A and Fig. S5a–c). We have previously demonstrated that SW 1353 cells are sensitive to the epigenetic BET inhibitor JQ1 and that, treatment with JQ1 not only inhibited YAP1 expression but also induced a significant upregulation of p21 expression⁴⁶. Herein, we found that the knockdown of YAP1 further enhanced the cellular senescence induced by JQ1 (Fig. 6B, C). Nevertheless, overexpression of HA-YAP-5SA suppressed the induction of p21 and prevented the cellular senescence induced by JQ1 treatment (Fig. 6D, E). Next, we sought to determine whether p21 also played a role in JQ1-mediated cellular senescence. As expected, loss of p21 by the siRNAs obviously suppressed the cellular senescence triggered by JQ1 in the SW 1353 cells (Fig. 6F, G). Mechanistically, p21 depletion was found to facilitate cell apoptosis by the activation of DNA damage response (DDR) via γ-H2AX and subsequent modulation of the Bcl-XL/Caspase-3 balance (Fig. 6H, I). These results together suggest that the YAP1/p21 axis is required for chemotherapy (e.g., JQ1)-induced cellular senescence.

**Fig. 6: The YAP1/p21 signaling axis is involved in chemotherapy-mediated cell senescence regulation.**

Accumulated evidence suggests that a delayed exit of senescent cells induced by first-line chemotherapy supports the tumor environment, which in turn leads to cancer relapse^29,44. We thus examined whether senolytic molecules, which recently have been developed to suppress cellular senescence by targeting the Bcl-2 family (e.g., Bcl-XL, Bcl-2, and Bcl-W)¹⁵, can eliminate YAP1 depletion-induced senescent cells. As shown in Fig. 7A–C, the inhibition in senescent cells by ABT263 (a senolytic inhibitor extensively used in clinical trials) was negatively correlated with an increase of cell death in a manner depending on the concentrations of ABT263 in the shYAP1#4 cells (Fig. 7A–C).

**Fig. 7: Knockdown of p21 expression enhances DNA damage caused by YAP deletion, induces apoptosis, and weakens cell senescence.**

Next, we hypothesized that sequential ABT263 treatment may enhance the therapeutic efficacy of JQ1. To test this, senescence was induced by JQ1 for 24 h, followed by the treatment with ABT263 for another 24 h before counting the cell number. As expected, ABT263 treatment significantly suppressed senescent CHS cells, accompanied by a reduction in viable cells (Fig. 7D–F). Furthermore, we found that treatment with ABT263 downregulated the expression of Bcl-XL and p21, while activated the caspase-3 and PARP (Fig. 7G). These results indicate that ABT263 might dictate senescent cells to undergo a caspase-3-dependent apoptotic cell death. Taken together, our results suggest that sequentially targeting the YAP1-p21 axis is a one-two punch approach that leads to more efficient senescent cell eradication (Fig. 7H).

Discussion

YAP1 is a critical mechanosensitive transcriptional regulator that controls skeletal development and cartilage-related disease⁴⁷. YAP1 is overexpressed in a series of sarcomas, including chondrosarcoma^5,48,49. However, the functional role of YAP1 in CHS is ill-defined. Our evidence supports the conclusion that YAP1 is critical for modulating the oncogenic phenotypes of CHS. First, expression of YAP1 is negatively associated with the differentiation status of CHS, as poorly differentiated CHS shows higher expression of YAP1. Second, knockdown of YAP1 greatly suppressed cell proliferation, induced cellular senescence in CHS cells, and reduced the growth of xenograft tumors. Of note, the impact of YAP1 on cellular senescence is still controversial, as some studies have demonstrated that depletion of YAP is sufficient to cause senescence-associated phenotypes in mesenchymal stem cells or breast cancer cells^11,50, while others have found that hyperactivation of YAP1 induces senescence in cultured primary human ovarian surface epithelial cells¹³. These conflicting results of YAP1 in regulating senescence appear to be highly dependent on different cancer settings or cell context-dependent manner. Third, in agreement with previous reports that nuclear YAP1 is critical for cell growth in various cancers, including lung, breast, and prostate cancers, we observed that YAP1 is predominately localized in the nucleus of CHS cells, and that nuclear localization of YAP1 (5SA) induces an oncogenic phenotype in primary chondrocytes and reverses YAP1 depletion-induced senescence in CHS cells. Fourth, It has been demonstrated that nuclear YAP1, via interaction with different transcription factors, including ErbB4, Runx2, NF-Y, mutantp53, p73, and the TEAD family members, is essential for activating many genes related to cell proliferation and antiapoptosis effects¹⁰. Our results herein also declared a requirement for TEAD in YAP1-regulated cellular senescence. Consistent with the above finding that YAP1 plays a critical role in regulating CHS growth, a recent study also demonstrates that YAP1 signaling is essential for the development of myxoid liposarcoma by driving the FUS-DDIT3 fusion gene. More importantly, this study reveals that the FUS-DDIT3 fusion gene physically associates with YAP1 in the nucleus, thus promoting its nuclear localization and elevating transcriptional activity⁵¹. Although nuclear-localized YAP1 has been demonstrated to cooperate with mutant p53 thus potentiating mutant p53’s gain-of-function activity, our results indirectly suggest that the p53 status might not be the key driver for YAP1-regulated cellular senescence as we had tested wild-type and mutant-type p53 harboring cells. Therefore, unbiased screening of interacting proteins or modifications that mediate YAP1 nuclear localization is of significance in delineating the underlying mechanism of YAP1 implication in CHS.

Two canonical pathways, the p53/p21 and pRb/p16 axes, have been implicated in cellular senescence triggered by the vast majority of stimuli. We have previously found that p21 induction and YAP1 downregulation were concurrently occurred in CHS treated with the BET inhibitor JQ1⁴⁶. In the present study, we were able to demonstrate that p21, but not p53, is fundamental for YAP1-regulated CHS cell senescence. This finding was then generalized to other sarcoma cells including U2 OS cells, as demonstrated by the colony formation and SA-β-gal assay. Since increasing studies have revealed that p21 can be regulated at transcriptional, translational, and posttranslational levels³⁷. p21 was first demonstrated to be a downstream target of p53 in vivo in response to DNA-damaging agents³⁸. Later, it was found that several other transcription factors, such as Sp1, AP2, and STAT, can also regulate p21 expression^36,37. In adult frog retinas, YAP1 knockdown was found to cause an upregulation of p21 expression, which involves p53 signaling⁵². In addition to the transcriptional regulation of p21 by YAP1, a recent study has reported that p21 is regulated by SKP2 in a YAP1-dependent manner²⁸. Our findings extend current knowledge on the regulatory mechanisms of YAP1 in p21 expression, which is the miR-17 family, miR-93 in particular, in crucial for YAP1-regulated p21 expression and subsequent cellular senescence in CHS. Several evidences have elucidated that YAP/TAZ is involved in miRNA biogenesis and processing through the modulation of Dicer and p72 microprocessor activity^14,42, thus it will be of interest to examine whether other members or clusters are involved in this regulatory axis in the context of senescence.

Increasing evidence has suggested that p21-mediated cellular senescence functions as a tumor-suppressive mechanism at the early stages of exposure to exogenous and endogenous stresses, including various chemotherapeutic drugs¹⁶. Whereas, prolonging senescence and p21 expression may elicit its detrimental effects, for instance, promoting cancer recurrence^20,22. Senescent cells increase the expression and secretion of numerous cytokines, chemokines, and other proteins thus creating a chronic inflammatory microenvironment that favors cancer cell metastasis and tumor recurrence¹⁸. In addition, recent studies have suggested that the accumulation of senescent cells in tissues and organs drives secondary tumors or cancer relapse^16,17. Our results demonstrate that the signaling axis of YAP1-p21 is responsive to a variety of chemotherapeutic treatments (cisplatin, etoposide, and JQ1) in different CHS cell lines, suggesting a universal mechanism during the chemotherapy. Therefore, eliminating senescent cells in a precise way enables the maximization of therapeutic efficacy. On the other hand, targeting the YAP1/TEAD expression and their transcriptional activity by BET inhibitors are proposed as promising strategies to overcome chemo-resistance^53,54.

A recent paper has reported that the inhibition of BET by JQ1 can suppress senescence immune surveillance via its association with super-enhancers⁵⁵. As a result, senescent cells induced by BET inhibition may not be cleared as early as possible, which eventually leads to drug resistance or tumor recurrent. Having observed that the expression of Bcl-XL inhibits the apoptosis of JQ1 herein and previously⁴⁶, we then proposed that inhibition of Bcl-XL may enhance the elimination of senescent cells. Altogether, we demonstrated that sequential targeting of YAP1 and senescence achieves maximum killing efficiency in CHS cells. A working model was also illustrated based on our findings (Fig. 7H), in which, after a first-line therapy (targeting YAP1 or using chemotherapeutic agents or the BET inhibitor), the damaged DNA will dictate cells to undergo senescence; at the second-line therapy, directly targeting p21 expression or using the senolytic agent ABT263 is essential for triggering senescent cells to undergo apoptosis in patients with compromised immune systems that are incapable of clearing senescent cells from the tumor microenvironment.

In summary, our study unveils a previously undescribed role of YAP1 signaling in mediating CHS cell senescence both in vitro and in vivo and demonstrates that the miR-17 family is critical for YAP1-regulated p21 expression and subsequent cellular senescence. Furthermore, we propose a “one-two punch” therapeutic approach by sequentially targeting YAP1 and p21, which maximizes therapeutic efficacy by enhancing the elimination of senescent cells.

Materials and methods

Cell culture and reagents

CHON-001, SW 1353, Hs 819.T, and U2 OS cells were obtained from the American Type Culture Collection (ATCC, VA, USA) and authenticated by a polymerase chain reaction of short tandem repeat (STR) sequences, as we described previously⁴⁶. CHON-001, SW 1353, and Hs 819.T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, cat# C12430500BT, USA). U2 OS cells were cultured in McCoy’s 5 A medium (Life Technologies, cat# 12330031, USA). Primary chondrocytes were isolated from patients who underwent total knee arthroplasty under a signed informed consent form. Patients with bone tumors, tuberculosis, infection, rheumatism, gout were excluded. The cartilage from the femoral condyle was digested with 0.25% trypsin (Thermo Fisher Scientific, cat# 25200072, USA) for 1 h, followed by digestion with type II collagenase (Thermo Fisher Scientific, cat# 17101015, USA) for 4 h at 37 °C. After filtering, the primary chondrocytes were cultured in DMEM. All culture media were supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and 1% penicillin/streptomycin. JQ1 (HY-13030) and Z-DEVD-FMK (HY-12466) were purchased from MedChem Express (Monmouth Junction, NJ, USA) and diluted with DMSO to a stock concentration of 100 mM. Verteporfin (cat# S1786) was obtained from Selleck Chemicals (Houston, TX, USA), and a working concentration of 1 µM was used to disrupt the YAP1-TEAD interaction. Etoposide (ETO, cat# S1225), cisplatin (CP, cat# S1166), MG132 (cat# S2619), and ABT263 (cat# S1001) were purchased from Selleck Chemicals (Houston, TX, USA). CP was diluted in DMF, and the others were diluted with dimethyl sulfoxide (DMSO) unless indicated otherwise. The same volume of DMSO was added and served as an internal control for treatment with inhibitors. Lipofectamine 2000 transfection reagent was purchased from Invitrogen (Thermo Fisher Scientific, cat# 11668019, USA).

Cell viability assay

SW 1353 cells were seeded in 96-well plates at a density of 2000 cells/well and then treated with different concentrations of JQ1 for various times. Cell viability was determined by Cell Counting Kit-8 (CCK-8, Beyotime, cat# C0042, China) according to the manufacturer’s instructions. The optical densities (ODs) were measured at a 450 mm wavelength with a multimode microplate reader (TriStar LB 941, Berthold, Germany).

Cell cycle analysis

Stable cells were cultured for 3 days before harvesting. Cell cycle progression was examined using a cell cycle detection kit (KeyGen Biotech, KGA512, China) by C6 flow cytometer (BD Biosciences, CA, USA), and the relative distributions of the G1, S, and G2 phases were analyzed by FlowJo 7.6.5 software (Tree Star Inc., Ashland, OR, USA).

Cell mechanical property evaluation

The elastic modulus, adhesion, and morphology of living cells were determined in contact mode by atomic force microscopy (AFM, BioScope Catalyst, Bruker Instruments, Germany). First, the cells were fixed with 4% paraformaldehyde (Beyotime, cat# P0099, China) for 15 min, washed three times with phosphate-buffered saline (PBS) for 3–5 min and immersed in PBS. The AFM force volume model was employed to measure the adhesion force and elastic modulus. The FV image was captured in the cytoplasmic region with a scan size of 5 × 5 μm, and more than 1000 force curves for each separate experiment were analyzed. The basic scan parameters were as follows: the ramp size was set as 1.5 μm, the forward and retraction velocity was 500 nm/s, and the spring constant of the AFM tip was 0.14380 N/m. Force curves were fit to the Sneddon model to estimate the elastic modulus. The experimental force-indentation curves were fitted with the formula

$$F = \frac{2}{\pi }\frac{E}{{\left( {1 - \nu } \right)^2}}{\mathrm{tan}}\left( \alpha \right)\delta ^2$$

where F, E, δ, α, and ν are the loading force, elastic modulus, depth of indentation, tip half angle of 18°, and Poisson’s ratio of 0.5, respectively.

Ethynyl deoxyuridine (EdU) incorporation assay

To detect DNA synthesis, cells were seeded in a 12-well plate at a density of 5 × 10⁴ cells/well and cultured for 72 h. Then the cells were incubated with 20 µM EdU pretreatment solution for another 2 h, followed by fixation with 4% paraformaldehyde. Then, EdU staining and Hoechst 33342 staining were performed using the Cell-Light EdU Apollo 567 In Vitro Imaging Kit (EdU, RiboBio, cat# C10310-1, China) according to the instructions. The ratio of EdU-positive cells/total cells (Hoechst 33342-positive cells) was calculated from ten random fields from three independent experiments.

Colony formation assay

Cells were seeded into 6-well plates at a density of 1000 cells per well and allowed to adhere in complete DMEM for eight days. Then, the colonies were washed with PBS and stained with crystal violet solution (Beyotime, cat# C0121, China). The relative number of colonies that contained more than 50 cells was counted under the SZ760 series microscope (Chongqing Optec Instrument Co., Ltd., China). Each experiment was performed in duplicate and repeated three times.

Quantitative real-time reverse-transcriptase polymerase chain (qRT-PCR) reaction

Total RNA was isolated using the PureLink RNA Mini Kit (Thermo Fisher Scientific, cat# 12183018 A, USA). The RNA concentration was determined with a NanoDrop system (NanoDrop Technologies Inc., USA) and then reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, cat# 4368814, USA). Specific primer sets for amplifying target genes were listed in Table S2. Target gene expression was calculated based on the ∆∆Ct method and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a reference gene. For miRNA quantification, the miRcute miRNA Isolation Kit (Tiangen Biotech, DP501, China) was used to isolate the miRNA according to the manufacturer’s instructions. One hundred nanograms of miRNA was used for reverse transcription according to the miRcute Plus miRNA First-Strand cDNA Kit (Tiangen Biotech, KR211, China). The expression level of miRNA was quantified with the miRcute Plus miRNA qPCR Kit (Tiangen Biotech, FP411, China). The specific forward primers were listed in Table S5. The 2-ΔΔCt method was used to determine the miRNA expression level. All PCRs were performed in triplicate on the DNA engine CFX96 Real-Time PCR amplification system (BIO-RAD, CFX96 Touch, Germany).

Preparation of nuclear and cytoplasmic protein extracts

A cell fractionation assay was conducted using an NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, cat# 78835, MA, USA) according to the manufacturer’s instructions. Briefly, cells were harvested, washed with PBS and then incubated with ice-cold cytoplasmic extraction reagents (CER) I for 10 min. After incubation, the CER II extraction reagent was added, followed by centrifugation for 5 min at maximum speed in a microcentrifuge (Heal Force, Neofuge 13R, China) (16,000 × g). The supernatant (cytoplasmic extract) was transferred to a clean prechilled tube, while the insoluble (pellet) fraction was resuspended with nuclear extraction reagents (NER) and incubated for a total of 40 min on ice. The supernatant after centrifugation at 16,000 × g for 10 min was then collected as the nuclear extract.

Immunoblots

Total protein lysate was prepared as we described previously⁵⁶. Cytoplasmic and nuclear proteins were extracted using a nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions. Briefly, aliquots of each sample containing 20~50 µg of protein were separated by electrophoresis in 8–15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Beyotime, cat# P0012A, China), and transferred to polyvinylidene fluoride membranes (PVDF, PALL, cat# BSP0161, MA, USA). After blocking with non-fat dry milk for 1 h at room temperature, the membranes were probed with primary antibodies obtained from Cell Signaling Technology (CST, MA, USA) or Abcam (UK): anti-YAP1 (CST, cat# 14074, USA, 1:1000), anti-HA (CST, cat# 2367, USA, 1:1000), anti-Cyr61 (CST, cat# 14479, USA, 1:1000), anti-Sp1 (CST, cat# 9389, USA, 1:1000), anti-p16 (Abcam, cat# ab51243, UK, 1:1000), anti-Dicer (CST, cat# 5362, USA, 1:1000), anti-γ-H2A.X (CST, cat# 9718, 1:1000), anti-p-p65 (CST, cat# 3033, USA, 1:1000), anti-caspase-3 (CST, cat# 9664, USA, 1:1000), anti-PARP (CST, cat# 9532, USA, 1:1000), anti-TAZ (CST, cat# 8418, USA, 1:1000), anti-β-actin (CST, cat# 3700, USA, 1:2000), anti-p53 (CST, cat# 9282, USA, 1:1000), anti-c-Myc (CST, cat# 13987, USA, 1:1000), anti-Bcl-xL (CST, cat# 2764, USA, 1:1000), anti-p21 (CST, cat# 2947, USA, 1:1000), anti-β-Tubulin (CST, cat# 2128, USA, 1:5000), and anti-Lamin-A/C (CST, cat# 2032, USA, 1:5000). Next, the membranes were incubated with HRP-linked anti-mouse IgG (CST, cat# 7076; 1:1000) or HRP-linked anti-rabbit IgG (CST, cat# 7074; 1:1000) secondary antibodies (Cell Signaling Technology, USA), and the blots were detected using Clarity Western ECL Substrate (BIO-RAD, cat# 1705061, Germany). The images were captured using a Tanon 5200 Luminescent Imaging Workstation (Tanon, China), and analyzed using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

Plasmid construction

YAP1 cDNA was gifted by Prof. Ximei Wu (Zhejiang University) and was subcloned in-frame into the HA-CMV vector using two enzymatic sites, BglII and NotI. A series of constructs encoding HA-YAP1-5SA, HA-YAP1-5SA-S94A, and mutants were generated by using ligation PCR, as we described previously⁵⁷. Primers used for HA-YAP1-5SA (five mutated phosphorylation sites) and HA-YAP1-5SA-S94A are listed in Table S4. All of the constructs were verified by sequencing and checked for expression.

Single-cell gel electrophoresis

The DNA Damage Detection Kit was purchased from KeyGen Biotech (KGA240-50, China), and the experiments were conducted according to the manufacturer’s instruction, with minor revision as we described previously⁵⁸. In brief, the cells were resuspended in PBS and allowed for solidification at 4 °C for 20 min on slides. The comet slides were then immersed in prechilled lysis buffer from the kit and washed twice with PBS. To assess DNA double-strand breaks (DSBs), migration was performed under alkaline conditions (pH = 12.3). After migration, the slides were washed in 0.4 mM Tris-HCl buffer (pH = 7.5) at 4 °C three times. Then, the slides were stained with propidium iodide (PI) in a dark box. The damaged DNA was detected by a Leica DMi8 fluorescence microscope (Leica Microsystems, Germany) at a 515~560 nm excitation wavelength. The OTM was analyzed by OpenComet software⁵⁹.

RNA interference

siRNAs targeting four different sequences of p53 and p21 (Gene Pharma, China) were used to perform the knockdown experiments. The four different sequences were listed in Table S2. For transient knockdown experiments, cells were seeded into a 6-well plate (Corning, Costar, cat# 3516, USA) for 24 h, and then the siRNAs were transfected with Lipofectamine 2000 (Thermo Fisher Scientific, cat# 11668019, USA) for 4 h in 1 mL of FBS free medium. Then, a complete medium was added, and the cells were cultured for another 72 h.

shRNA construction and establishment of stable shYAP1 cell lines

Lentivirus particles expressing shYAP1 were produced by GenePharma Co., Ltd. (Shanghai, China). Briefly, a series of YAP1 targeting sequences were selected and cloned into a lentivirus-based RNAi system (pGLV3/H1/GFP + Puro Vector, GenePharma). shRNA target sequences were listed in Table S1. The lentiviral shRNA-expression plasmids (SC or shY#4) were transfected with the packaging plasmids into 293T cells for lentivirus generation. Supernatants containing lentivirus were then harvested and concentrated. SW 1353 cells were then infected by applying viral supernatant (multiplicity of infection, MOI = 20) in 10 mL of complete medium for 24 h, and the stable cell lines were selected using 2 μg/mL puromycin for 3 days for two passages. Puromycin (1 μg/mL) was used to maintain the cells.

High-throughput sequencing and bioinformatics analysis

High-throughput sequencing was completed by RiboBio Co. Ltd. (Guangzhou, China). The operation procedure can be briefly described as follows. Total RNA was extracted, purified and confirmed for quantity and integrity, and then 1 μg of total RNA from each sample was used to prepare small RNA libraries with the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) according to the manufacturer’s instructions. The libraries were used for sequencing on the Illumina HiSeqTM 2500 sequencing platform (Illumina, San Diego, CA, USA). Bioinformatics analysis of raw sequence data was conducted, and the ensemble transcript database was used to annotate the results. TargetScan (http://www.targetscan.org/), miRDB (http://mirdb.org/), miRWalk (http://mirwalk.umm.uni-heidelberg.de) and miRbase (http://www.mirbase.org/) were applied to predict the target genes of the selected miRNAs.

miRNA transfection

The miR-93 mimic control, hsa-miR-93 mimic (cat# miR10000093-1-5), miR-93 inhibitor control, and hsa-miR-93 inhibitor (cat# miR20000093-1-5) were purchased from RiboBio (Guangzhou, China). For miRNA transfection, SW 1353 cells were seeded into a 6-well plate for 24 h, and 5 μL of miR-93 mimic or 2.5 μL of miR-93 inhibitor was transfected with 6 μL of Lipofectamine 2000 for 4 h in 1 mL of FBS free medium. Then, a complete medium was added and cells were cultured for another 72 h.

Senescence-associated β-galactosidase staining

Senescence-associated β-galactosidase (SA-β-gal) staining was performed using a commercial kit (Beyotime, C0602, China) according to the manufacturer’s instructions. Briefly, cells were grown in 6-well plates at a density of 1 × 10⁵/well and tissue sections were made from frozen sections of tumor tissue peeled from nude mice. After treatment, the cells or tissue sections were fixed with β-galactosidase fixative solution for 15 min, followed by staining with SA-β-gal staining solution at pH 6.0 and 37 °C for 6–24 h (depending on the cell line). The senescent cells were observed and captured with an Olympus IX51 (Olympus, Tokyo, Japan). The percentage of SA-β-gal-positive cells was quantified from at least three random fields per sample (a minimum of 300 cells) and calculated as: (β-gal-positive cells/total cells in a field) × 100%.

Tissue microarray and immunohistochemistry (IHC)

OS803 chondrosarcoma tissue microarray was purchased from US Biomax, Inc. The staining was conducted by pathologists, and the scoring was performed as previously reported⁶⁰. Briefly, slides were deparaffinized in xylene and rehydrated through a graded alcohol series before endogenous peroxidase activity was blocked with 3% H₂O₂ in methanol. Antigen retrieval was achieved by incubating the slides in Citrate buffer at 60 °C for 20 min, followed by blocking with goat serum to avoid nonspecific binding. The primary anti-YAP1 antibody (Novus Biologicals, cat# NB110-58358, USA, 1:100), c-myc (Abcam, cat# ab32072, UK, 1:100), Ki67 (Servicebio, cat# GB111499, China, 1:200), p21 (CST, cat# 2947, USA, 1:50) were added overnight at 4 °C. Then the samples were incubated with HRP-conjugated secondary antibodies (CST, cat# 7074, 1:100) at room temperature for another 1 h, followed by counterstaining with hematoxylin. All images were acquired using a light microscope (Leica, 390335, Germany). The semi-quantification of YAP1 expression was performed as follow: The intensity was scored as 0 (no expression), 1 (low expression), 2 (moderate expression), and 3 (high expression), and the percentage of cells showing the expression was scored ranging from 0 to 10 with 10 as the highest percentage (100%). The expression score for cytoplasmic- and nuclear-localized YAP1 was respectively determined by the intensity score times the percentage (0–30), and the total expression score is the sum of the cytoplasmic and nuclear expression scores (0–60).

Immunofluorescence (IF)

Cells were seeded onto coverslips precoated with poly-L-lysine and then cultured for 72 h. The cells were washed with PBS and then fixed with 4% polyformaldehyde and permeabilized with 0.2% Triton X-100. After blocking with 5% nonfat milk for 30 min, the cells were incubated with antibodies against YAP1 (CST, cat# D8H1X, 1:100, USA), 53BP1 (CST, cat# E7N5D, 1:200, USA), γ-H2AX (CST cat# 20E3, 1:100, USA) and F-actin (Invitrogen, cat# MA1-80729, 1:100, USA) for 2 h at RT. After three washes with TBST, the cells were incubated with anti-rabbit IgG (H + L), F(ab’)2 Fragment Alexa Fluor® 488 Conjugate (CST, cat# 4412, 1:100, USA), anti-rabbit IgG (H + L), F(ab’)2 Fragment Alexa Fluor® 555 Conjugate (CST, cat# 4413, 1:100 USA) or anti-mouse IgG (H + L), F(ab’)2 Fragment Alexa Fluor® 555 Conjugate (CST, cat# 4409, 1:100, USA) for 1 h in the dark and then washed three times as described above. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, cat# 32670, USA). The images were captured under a confocal microscope (Carl Zeiss, Zeiss LSM 700, Germany) with a ×63 objective lens. The percentage of foci of 53BP1 and γ-H2AX was automatically counted with ImageJ using the ITCN plugin in 10 fields for a total of more than 100 nuclei.

Xenograft tumor growth in nude mice

The animal experiments were approved by the Jinan University Animal Care and Use Committee and conformed to the “Guide for the Care and Use of Laboratory Animals” of the National Institute of Health in China. Five- to six-week-old BALB/c nu/nu mice were purchased from the Model Animal Research Center of Nanjing University (Nanjing China). SW 1353 cells infected with shYAP1 (shYAP#3, shYAP#4) or SC were harvested and then washed with PBS three times. Then, the cells were resuspended at 1 × 10⁷ cells/mL in prechilled PBS-diluted Matrigel (BD Biosciences, cat# 354248, USA, 1:1 by volume). Then, 0.2 mL of the resuspended cells were randomly injected subcutaneously into the flanks of mice and allowed to grow for ~1 month. At the end of the experiments, the tumors were resected (N = 5), formalin fixed, and paraffin embedded. For p21 inhibition experiments, xenograft tumors were established by subcutaneously inoculating the SC and shYAP1#4 stable cells into the nude mice at the right flank next to the forelimb. Each group of nude mice transplanted with the SW1353 cells was randomly divided into DMSO and UC2288 (p21 inhibitor; MERCK, cat# 5.32813.0001, USA) treated group. UC2288 was dissolved in DMSO with a concentration of 5 mg/mL and was intraperitoneal injected with 10 mg/kg for four times within 7 days. Tumor growth was monitored every 3 days, and the tumor volume was calculated using 1/2 × L × W × H. All of the sections (4 µm) were cut and stained with hematoxylin and eosin. Frozen section was prepared for cellular senescence analysis. For IHC staining, sections were washed with PBS, developed using a DAB kit (CST, cat# 8059, USA) and then counterstained with hematoxylin. All of the IHC results were scanned by Pannoramic MIDI (3DHISTECH, Hungary). For the determination of cell apoptosis in tissues, TUNEL Apoptosis Detection kit (Yeasen, cat# 40308, China) was used according to the manufacturer’s instructions.

Statistical analysis

Statistical analysis was performed using GraphPad Prism (GraphPad Software, San Diego, CA, USA). All of the results are shown as the mean ± standard deviation (SD). Data were reported as biological replicates, with technical replicates indicated in the Figure legends. The student’s t-test was used to compare values of test and control samples. One-way analysis of variance (ANOVA) was used for multiple group comparison, followed by Dunnett’s test. Two-way ANOVA was applied to compare the means of two independent variables, followed by Tukey’s test. Significance levels of P < 0.05, < 0.01, < 0.001, < 0.0001 were denoted in graphs by different asterisks.

References

Chow, W. A. Chondrosarcoma: biology, genetics, and epigenetics. F1000Res. 7, 1826–1835 (2018).
Mery, B. et al. Biological aspects of chondrosarcoma: leaps and hurdles. Crit. Rev. Oncol. Hematol. 126, 32–36 (2018).
Article PubMed Google Scholar
van Oosterwijk, J. G. et al. Restoration of chemosensitivity for doxorubicin and cisplatin in chondrosarcoma in vitro: BCL-2 family members cause chemoresistance. Ann. Oncol. 23, 1617–1626 (2012).
Article PubMed Google Scholar
Kim, D. W. et al. Targeting of cell survival genes using small interfering RNAs (siRNAs) enhances radiosensitivity of Grade II chondrosarcoma cells. J. Orthop. Res. 25, 820–828 (2007).
Article CAS PubMed Google Scholar
Fullenkamp, C. A. et al. TAZ and YAP are frequently activated oncoproteins in sarcomas. Oncotarget 7, 30094–30108 (2016).
Article PubMed PubMed Central Google Scholar
Eisinger-Mathason, T. S. et al. Deregulation of the Hippo pathway in soft-tissue sarcoma promotes FOXM1 expression and tumorigenesis. Proc. Natl Acad. Sci. USA 112, E3402–E3411 (2015).
Article CAS PubMed PubMed Central Google Scholar
Van Mater, D. et al. Injury promotes sarcoma development in a genetically and temporally restricted manner. JCI Insight 3, e123687–e123698 (2018).
Totaro, A., Panciera, T. & Piccolo, S. YAP/TAZ upstream signals and downstream responses. Nat. Cell Biol. 20, 888–899 (2018).
Article CAS PubMed PubMed Central Google Scholar
Zhao, B. et al. Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Genes Dev. 21, 2747–2761 (2007).
Article CAS PubMed PubMed Central Google Scholar
Mohri, Z., Del Rio Hernandez, A. & Krams, R. The emerging role of YAP/TAZ in mechanotransduction. J. Thorac. Dis. 9, E507–E509 (2017).
Article PubMed PubMed Central Google Scholar
Santinon, G. et al. dNTP metabolism links mechanical cues and YAP/TAZ to cell growth and oncogene-induced senescence. EMBO J. 37, e97780–e97795 (2018).
Xie, Q. et al. YAP/TEAD-mediated transcription controls cellular senescence. Cancer Res. 73, 3615–3624 (2013).
Article CAS PubMed Google Scholar
Cui, Z. et al. The Receptor for Hyaluronan-Mediated Motility (CD168) promotes inflammation and fibrosis after acute lung injury. Matrix Biol. 78, 255–271 (2019).
Article PubMed CAS Google Scholar
Mori, M. et al. Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. Cell 156, 893–906 (2014).
Article CAS PubMed PubMed Central Google Scholar
He, S. & Sharpless, N. E. Senescence in health and disease. Cell 169, 1000–1011 (2017).
Article CAS PubMed PubMed Central Google Scholar
Sieben, C. J., Sturmlechner, I., van de Sluis, B. & van Deursen, J. M. Two-step senescence-focused cancer therapies. Trends Cell Biol. 28, 723–737 (2018).
Article CAS PubMed PubMed Central Google Scholar
Goy, E. & Abbadie, C. Senescence and cancer: double-dealing. Med. Sci. 34, 223–230 (2018).
Google Scholar
Lee, S. & Schmitt, C. A. The dynamic nature of senescence in cancer. Nat. Cell Biol. 21, 94–101 (2019).
Article CAS PubMed Google Scholar
Campo-Trapero, J. et al. Cellular senescence in oral cancer and precancer and treatment implications: a review. Acta Oncol. 47, 1464–1474 (2008).
Article CAS PubMed Google Scholar
Georgakilas, A. G., Martin, O. A. & Bonner, W. M. p21: A two-faced genome guardian. Trends Mol. Med. 23, 310–319 (2017).
Article CAS PubMed Google Scholar
Galanos, P. et al. Chronic p53-independent p21 expression causes genomic instability by deregulating replication licensing. Nat. Cell Biol. 18, 777–789 (2016).
Article CAS PubMed PubMed Central Google Scholar
Yosef, R. et al. p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling. EMBO J. 36, 2280–2295 (2017).
Article CAS PubMed PubMed Central Google Scholar
Guo, L. & Teng, L. YAP/TAZ for cancer therapy: opportunities and challenges (review). Int. J. Oncol. 46, 1444–1452 (2015).
Article CAS PubMed Google Scholar
Evans, H. L., Ayala, A. G. & Romsdahl, M. M. Prognostic factors in chondrosarcoma of bone: a clinicopathologic analysis with emphasis on histologic grading. Cancer 40, 818–831 (1977).
Article CAS PubMed Google Scholar
Ma, L. G. et al. LKB1 inhibits the proliferation of gastric cancer cells by suppressing the nuclear translocation of Yap and beta-catenin. Int J. Mol. Med. 37, 1039–1048 (2016).
Article CAS PubMed Google Scholar
Vlug, E. J. et al. Nuclear localization of the transcriptional coactivator YAP is associated with invasive lobular breast cancer. Cell Oncol. 36, 375–384 (2013).
Article CAS Google Scholar
Hayashi, K. & Iwata, M. Stiffness of cancer cells measured with an AFM indentation method. J. Mech. Behav. Biomed. Mater. 49, 105–111 (2015).
Article PubMed Google Scholar
Jang, W., Kim, T., Koo, J. S., Kim, S. K. & Lim, D. S. Mechanical cue-induced YAP instructs Skp2-dependent cell cycle exit and oncogenic signaling. EMBO J. 36, 2510–2528 (2017).
Article CAS PubMed PubMed Central Google Scholar
Childs, B. G., Baker, D. J., Kirkland, J. L., Campisi, J. & van Deursen, J. M. Senescence and apoptosis: dueling or complementary cell fates? EMBO Rep. 15, 1139–1153 (2014).
Article CAS PubMed PubMed Central Google Scholar
Huang, J., Wu, S., Barrera, J., Matthews, K. & Pan, D. The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP. Cell 122, 421–434 (2005).
Article CAS PubMed Google Scholar
Karimian, A., Ahmadi, Y. & Yousefi, B. Multiple functions of p21 in cell cycle, apoptosis and transcriptional regulation after DNA damage. DNA Repair 42, 63–71 (2016).
Article CAS PubMed Google Scholar
Shtutman, M., Chang, B. D., Schools, G. P. & Broude, E. V. Cellular model of p21-induced senescence. Methods Mol. Biol. 1534, 31–39 (2017).
Article CAS PubMed PubMed Central Google Scholar
Takeuchi, S. et al. TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase. Biochem Biophys. Res. Commun. 486, 385–390 (2017).
Article CAS PubMed Google Scholar
Campisi, J. & d’Adda di Fagagna, F. Cellular senescence: when bad things happen to good cells. Nat. Rev. Mol. Cell Biol. 8, 729–740 (2007).
Article CAS PubMed Google Scholar
Liu-Chittenden, Y. et al. Genetic and pharmacological disruption of the TEAD-YAP complex suppresses the oncogenic activity of YAP. Genes Dev. 26, 1300–1305 (2012).
Article CAS PubMed PubMed Central Google Scholar
Warfel, N. A. & El-Deiry, W. S. p21WAF1 and tumourigenesis: 20 years after. Curr. Opin. Oncol. 25, 52–58 (2013).
Article CAS PubMed Google Scholar
Abbas, T. & Dutta, A. p21 in cancer: intricate networks and multiple activities. Nat. Rev. Cancer 9, 400–414 (2009).
Article CAS PubMed PubMed Central Google Scholar
Macleod, K. F. et al. p53-dependent and independent expression of p21 during cell growth, differentiation, and DNA damage. Genes Dev. 9, 935–944 (1995).
Article CAS PubMed Google Scholar
Roninson, I. B. Oncogenic functions of tumour suppressor p21(Waf1/Cip1/Sdi1): association with cell senescence and tumour-promoting activities of stromal fibroblasts. Cancer Lett. 179, 1–14 (2002).
Article CAS PubMed Google Scholar
Karkhanis, M. & Park, J. I. Sp1 regulates Raf/MEK/ERK-induced p21(CIP1) transcription in TP53-mutated cancer cells. Cell Signal. 27, 479–486 (2015).
Article CAS PubMed PubMed Central Google Scholar
Jauhari, A. et al. Differentiation induces dramatic changes in miRNA profile, where loss of dicer diverts differentiating SH-SY5Y cells toward senescence. Mol. Neurobiol. 54, 4986–4995 (2017).
Article CAS PubMed Google Scholar
Chaulk, S. G., Lattanzi, V. J., Hiemer, S. E., Fahlman, R. P. & Varelas, X. The Hippo pathway effectors TAZ/YAP regulate dicer expression and microRNA biogenesis through Let-7. J. Biol. Chem. 289, 1886–1891 (2014).
Article CAS PubMed Google Scholar
Kim, Y. K., Kim, B. & Kim, V. N. Re-evaluation of the roles of DROSHA, Export in 5, and DICER in microRNA biogenesis. Proc. Natl Acad. Sci. USA 113, E1881–E1889 (2016).
Article CAS PubMed PubMed Central Google Scholar
Demaria, M. et al. Cellular senescence promotes adverse effects of chemotherapy and cancer relapse. Cancer Discov. 7, 165–176 (2017).
Article CAS PubMed Google Scholar
Ansieau, S. & Collin, G. Senescence versus apoptosis in chemotherapy. Oncotarget 6, 4551–4552 (2015).
Article PubMed PubMed Central Google Scholar
Zhang, H. T. et al. The BET bromodomain inhibitor JQ1 suppresses chondrosarcoma cell growth via regulation of YAP/p21/c-Myc signaling. J. Cell Biochem. 118, 2182–2192 (2017).
Article CAS PubMed Google Scholar
Deng, Y. et al. Reciprocal inhibition of YAP/TAZ and NF-kappaB regulates osteoarthritic cartilage degradation. Nat. Commun. 9, 4564 (2018).
Article PubMed PubMed Central CAS Google Scholar
Merritt, N. M. et al. A comprehensive evaluation of Hippo pathway silencing in sarcomas. Oncotarget 9, 31620–31636 (2018).
Article PubMed PubMed Central Google Scholar
Ye, S. et al. YAP1-mediated suppression of USP31 enhances NFkappaB activity to promote sarcomagenesis. Cancer Res. 78, 2705–2720 (2018).
Article CAS PubMed PubMed Central Google Scholar
Fu, L. et al. Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biol. 17, e3000201 (2019).
Article CAS PubMed PubMed Central Google Scholar
Goransson, M. et al. The myxoid liposarcoma FUS-DDIT3 fusion oncoprotein deregulates NF-kappaB target genes by interaction with NFKBIZ. Oncogene 28, 270–278 (2009).
Article CAS PubMed Google Scholar
Cabochette, P. et al. YAP controls retinal stem cell DNA replication timing and genomic stability. Elife 4, e08488 (2015).
Article PubMed PubMed Central Google Scholar
Reggiani, F., Gobbi, G., Ciarrocchi, A., Ambrosetti, D. C. & Sancisi, V. Multiple roles and context-specific mechanisms underlying YAP and TAZ-mediated resistance to anti-cancer therapy. Biochim Biophys. Acta Rev. Cancer 1873, 188341 (2020).
Article PubMed CAS Google Scholar
Sun, J. et al. A tightly controlled Src-YAP signaling axis determines therapeutic response to dasatinib in renal cell carcinoma. Theranostics 8, 3256–3267 (2018).
Article CAS PubMed PubMed Central Google Scholar
Tasdemir, N. et al. BRD4 connects enhancer remodeling to senescence immune surveillance. Cancer Discov. 6, 612–629 (2016).
Article CAS PubMed PubMed Central Google Scholar
Li, Y. H. et al. PRMT5-TRIM21 interaction regulates the senescence of osteosarcoma cells by targeting the TXNIP/p21 axis. Aging. 12, 2507–2529 (2020).
Zhang, H. T., Zhang, D., Zha, Z. G. & Hu, C. D. Transcriptional activation of PRMT5 by NF-Y is required for cell growth and negatively regulated by the PKC/c-Fos signaling in prostate cancer cells. Biochim Biophys. Acta 1839, 1330–1340 (2014).
Article CAS PubMed PubMed Central Google Scholar
Yang, J. et al. HSP90 regulates osteosarcoma cell apoptosis by targeting the p53/TCF-1-mediated transcriptional network. J. Cell Physiol. 235, 3894–3904 (2020).
Article CAS PubMed Google Scholar
Gyori, B. M., Venkatachalam, G., Thiagarajan, P. S., Hsu, D. & Clement, M. V. OpenComet: an automated tool for comet assay image analysis. Redox Biol. 2, 457–465 (2014).
Article CAS PubMed PubMed Central Google Scholar
Deng, X. et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene 36, 1223–1231 (2017).
Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank all the members of the Zha laboratory for their helpful comments on the manuscript. We thank Dr. Chang-Deng Hu at Purdue University for the critical discussion and proofreading the manuscript.

Funding statement

The authors acknowledge support from the National Natural Science Foundation of China (81602360, 81672224, and 81871809), the Natural Science Foundation of Guangdong Province (2019A1515011082, 2017A030313665, and 2017A030313556), the China Postdoctoral Science Foundation (2016M602606), and the China Postdoctoral Science Special Foundation (2017T100661), the Major Science and Technology Planning Projects of Tianhe District (2018YZ001), and the Medical Scientific Research Foundation of Guangdong Province (A2017485).

Author information

These authors contributed equally: Huan-Tian Zhang, Tao Gui

Authors and Affiliations

Institute of Orthopedic Diseases, Jinan University, Guangzhou, China
Huan-Tian Zhang, Tao Gui, Ri-Xu Liu, Chong-Jie Wu, Zhenyan Li, Qiu-Tong Xu, Jie Yang, Wang Tang, Ning Liu & Zhen-Gang Zha
Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan University, Guangzhou, China
Huan-Tian Zhang, Tao Gui, Ri-Xu Liu, Kui-Leung Tong, Chong-Jie Wu, Zhenyan Li, Qiu-Tong Xu, Jie Yang, Wang Tang, Ning Liu & Zhen-Gang Zha
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou, China
Huan-Tian Zhang, Wanting Liu & Qing-Yu He
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, China
Xun Huang
Department of Joint Replacement and Trauma Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
Yuan Sang
Department of Cell and Molecular Medicine, Rush University Medical Center, Chicago, IL, USA
Ryan D. Ross

Authors

Huan-Tian Zhang
View author publications
You can also search for this author in PubMed Google Scholar
Tao Gui
View author publications
You can also search for this author in PubMed Google Scholar
Ri-Xu Liu
View author publications
You can also search for this author in PubMed Google Scholar
Kui-Leung Tong
View author publications
You can also search for this author in PubMed Google Scholar
Chong-Jie Wu
View author publications
You can also search for this author in PubMed Google Scholar
Zhenyan Li
View author publications
You can also search for this author in PubMed Google Scholar
Xun Huang
View author publications
You can also search for this author in PubMed Google Scholar
Qiu-Tong Xu
View author publications
You can also search for this author in PubMed Google Scholar
Jie Yang
View author publications
You can also search for this author in PubMed Google Scholar
Wang Tang
View author publications
You can also search for this author in PubMed Google Scholar
Yuan Sang
View author publications
You can also search for this author in PubMed Google Scholar
Wanting Liu
View author publications
You can also search for this author in PubMed Google Scholar
Ning Liu
View author publications
You can also search for this author in PubMed Google Scholar
Ryan D. Ross
View author publications
You can also search for this author in PubMed Google Scholar
Qing-Yu He
View author publications
You can also search for this author in PubMed Google Scholar
Zhen-Gang Zha
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

Conception and design of the study: H.T.Z. Acquisition of most of the experimental results: T.G. Clinical specimen staining and animal study: N.L., R.X.L., Y.S., and C.J.W. Analysis and/or interpretation of data: H.T.Z. AFM study: T.K.L. and X.H. Construction of plasmids: Z.Y.L. and J.Y. Bioinformatics analysis: W.T.L. Senescence-related staining: W.T. and Q.T.X.: Writing and revision of the manuscript: H.T.Z. and R.R. Study supervision: Q.Y.H. and Z.G.Z. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Huan-Tian Zhang, Qing-Yu He or Zhen-Gang Zha.

Ethics declarations

Ethics statement

Tissue microarrays: The expression of YAP1 was analyzed using commercial tissue microarrays (TMAs) of chondrosarcoma (OS803, Biomax, Inc., US), which includes 80 sections of chondrosarcoma tissue samples. The company stated that all human tissues are collected under IRB (Institutional Review Board) and HIPPA approved protocols. The animal experiments were approved by the Jinan University Animal Care and Use Committee and conformed to the “Guide for the Care and Use of Laboratory Animals” of the National Institute of Health in China.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Edited by G. Blandino

Supplementary information

SUPPLEMENTAL MATERIAL

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Zhang, HT., Gui, T., Liu, RX. et al. Sequential targeting of YAP1 and p21 enhances the elimination of senescent cells induced by the BET inhibitor JQ1. Cell Death Dis 12, 121 (2021). https://doi.org/10.1038/s41419-021-03416-1

Download citation

Revised: 30 December 2020
Accepted: 05 January 2021
Published: 25 January 2021
DOI: https://doi.org/10.1038/s41419-021-03416-1

This article is cited by

Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling
- Ri-Xu Liu
- Rong-He Gu
- Huan-Tian Zhang
Bone Research (2023)
The interplay between noncoding RNA and YAP/TAZ signaling in cancers: molecular functions and mechanisms
- Yirao Zhang
- Yang Wang
- Keming Wang
Journal of Experimental & Clinical Cancer Research (2022)