Fig. 5: Tan IIA inhibits c-Myc expression in an Akt signaling dependent manner. | Cell Death & Disease

Fig. 5: Tan IIA inhibits c-Myc expression in an Akt signaling dependent manner.

From: Tanshinone IIA inhibits oral squamous cell carcinoma via reducing Akt-c-Myc signaling-mediated aerobic glycolysis

Fig. 5

a CAL27 cells were treated with Tan IIA for 24 h, WCE was subjected to IB analysis. b The sgCtrl and sgAkt stable expressing CAL27 cells were treated with Tan IIA for 24 h, WCE was subjected to IB analysis. c, d The sgCtrl, and sgAkt stable expressing CAL27 cells were treated with Tan IIA for 24 h. Cell culture medium was subjected to glucose consumption (c) and lactate production (d) analysis. e The sgCtrl and sgAkt stable expressing CAL27 cells were treated with Tan IIA for 24 h, WCE was subjected to co-IP analysis. f CAL27 cells were transfected with Myr-Akt1 and treated with Tan IIA for 24 h, WCE was subjected to IB analysis. g, h CAL27 cells were transfected with Myr-Akt1 and treated with Tan IIA for 24 h, the cell culture medium was subjected to glucose consumption (g) and lactate production (h) analysis. i CAL27 cells were transfected with Myr-Akt1 and treated with Tan IIA for 24 h, WCE was subjected to ubiquitination analysis. j CAL27 cells were transfected with Myr-Akt1 and treated with Tan IIA for 24 h, MTS assay was performed to analyze the cell viability. k, l CAL27 cells were transfected with Myr-Akt1 and treated with Tan IIA for 24 h, WCE was subjected to IB analysis (k) and caspase 3 activity analysis (l). ***p < 0.001.

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