Fig. 3: Tan IIA decreases the HK2 protein level and induces intrinsic apoptosis. | Cell Death & Disease

Fig. 3: Tan IIA decreases the HK2 protein level and induces intrinsic apoptosis.

From: Tanshinone IIA inhibits oral squamous cell carcinoma via reducing Akt-c-Myc signaling-mediated aerobic glycolysis

Fig. 3

a MTS analysis of the cell viability of OSCC cells treated with Tan IIA. b Trypan blue exclusion assay analysis of the population of live cells in Tan IIA-treated OSCC cells. c MTS analysis the cell viability of OSCC cells treated with various inhibitors and Tan IIA. d, e Caspase 3 activity (d), and the protein level of cleaved caspase 3 and -PARP (e) in Tan IIA-treated CAL27 cells. f Flow cytometry analysis of the population of apoptotic cells in Tan IIA-treated CAL27 cells. g IB analysis of HK2 expression in the mitochondrial fraction of Tan IIA-treated CAL27 cells. h CAL27 cells were treated with Tan IIA, subcellular fractions were isolated and subjected to IB analysis. i MTS assay analysis of cell viability of OSCC cells transfected with HK2 and treated with Tan IIA. j Trypan blue exclusion assay analysis of the population of live cells in HK2 transfected and Tan IIA-treated OSCC cells. k Caspase 3 activity in HK2 transfected and Tan IIA-treated CAL27 cells. l Flow cytometry analysis of the population of apoptotic cells in HK2 transfected and Tan IIA-treated CAL27 cells. m IB analysis of cleaved caspase 3 and -PARP in HK2 transfected and Tan IIA-treated CAL27 cells. n CAL27 cells were transfected with HK2 and treated with Tan IIA for 24 h. Subcellular fractions were isolated and subjected to IB analysis. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page