Fig. 6: Effects of anti-HMGB1 neutralizing antibody on the levels of extracellular Aβ released from H4swe cells treated with H/R-conditioned medium (CM) from HBMVECs. | Cell Death & Disease

Fig. 6: Effects of anti-HMGB1 neutralizing antibody on the levels of extracellular Aβ released from H4swe cells treated with H/R-conditioned medium (CM) from HBMVECs.

From: Sirtuin 1-dependent regulation of high mobility box 1 in hypoxia–reoxygenated brain microvascular endothelial cells: roles in neuronal amyloidogenesis

Fig. 6

HBMVECs were exposed to H/R for 1 h and reoxygenated for 24 h, and CM collected. This was treated with anti-HMGB1 antibody (0.25 or 0.5 μg/ml), and then H4swe cells were cultured with H/R CM with or without neutralization for 24 h. The concentrations of Aβ40 (a) and Aβ42 (b) in CM were analysed by ELISA. Data are presented as the mean ± SD from repeated independent experiments (n = 3–4). *p < 0.05, ***p < 0.001 by unpaired t-tests. HBMVECs with or without Sirt1 silencing were exposed to 24-h H/R, and then H4swe cells were treated with the endothelial CM with or without neutralization of HMGB1. The levels of Aβ40 (c) and Aβ42 (d) in H4swe CM were analysed by ELISA. Data are presented as mean ± SD from repeated independent experiments (n = 3–4). *p < 0.05, **p < 0.01 versus vehicle control; #p < 0.05, ##p < 0.01 versus IgG, $p < 0.05 versus the control scrambled siRNA group by unpaired t-tests.

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