Fig. 2: H/R activated JNK signalling and HMGB1 regulated c-Jun activation in HBMVECs. | Cell Death & Disease

Fig. 2: H/R activated JNK signalling and HMGB1 regulated c-Jun activation in HBMVECs.

From: Sirtuin 1-dependent regulation of high mobility box 1 in hypoxia–reoxygenated brain microvascular endothelial cells: roles in neuronal amyloidogenesis

Fig. 2

a HBMVECs were exposed to H/R for the indicated times. The expression levels of phosphorylated JNK (p-JNK(T183/Y185), arrows) and phosphorylated c-jun in total lysate, ICAM1 in the cytosolic fraction, and the translocation of p65 in nuclear fractions were analysed; α-tubulin and lamin B were used as loading controls. Representative blots from triplicated experiments are presented. b HBMVECs were transfected with scrambled siRNA (scramble) or siRNA targeting the HMGB1 gene (HMGB1 siRNA). After 24 h of transfection, HBMVECs were exposed to 24-h H/R or normoxia, and the level of intracellular p-c-Jun was analysed by western blotting; α-tubulin was used as a loading control, and representative blots are presented. Bar graph indicate the relative expression level of p-c-jun when compared with NC cells transfected scrambled siRNA (n = 4, mean ± SD; *p < 0.05 versus H/R scrambled siRNA group by unpaired t-test).

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