Fig. 1: Anlotinib inhibits ICC cell growth and invasion, promotes apoptosis in vitro. | Cell Death & Disease

Fig. 1: Anlotinib inhibits ICC cell growth and invasion, promotes apoptosis in vitro.

From: Anlotinib suppresses tumor progression via blocking the VEGFR2/PI3K/AKT cascade in intrahepatic cholangiocarcinoma

Fig. 1

a, b HCCC9810 and RBE cells were treated with anlotinib at the indicated concentrations for 24, 48, or 72 h. Cell viability was measured using CCK8, and IC50 values were calculated; c representative images of the clone formation assay; d the ratio of apoptotic cells was measured in RBE and HCCC9810 cells after anlotinib treatment for 48 h. Apoptosis was detected using Annexin V-FITC and propidium iodide (PI) staining; e cells were treated with anlotinib at the indicated concentration for 24 h and then stained with PI for flow cytometric analysis. Each experiment was performed in triplicate. *P < 0.05; **P < 0.01.

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