a Scatter plot of RNA-Seq analysis of LECs isolated from E15.5 control (n = 3) and Dot1l∆EC (n = 2) embryos. n = 2 and 3: pooled biological replicates per RNA-Seq library. Genes critical for lymph vessel formation and function are indicated. Red and blue dots indicate downregulated and upregulated genes in Dot1l∆EC LECs, respectively. b qRT-PCR analysis confirming reduced gene expression in E15.5 Dot1l∆EC dermal LECs compared with the wild type (n = 2/group). Error bars show mean ± s.e.m. c, d Gene Ontology (GO) term analysis and Gene Set Enrichment Analysis (GSEA) with genes downregulated in Dot1l∆EC LECs. Each red (repressed genes in cKO) and blue (upregulated genes in cKO) represents one gene in each GO term, respectively. NES normalized enrichment score, FDR false discovery rate.