Fig. 5: Dot1l loss impairs transcriptional program important for LEC development and function. | Cell Death & Disease

Fig. 5: Dot1l loss impairs transcriptional program important for LEC development and function.

From: Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function

Fig. 5

a Scatter plot of RNA-Seq analysis of LECs isolated from E15.5 control (n = 3) and Dot1l∆EC (n = 2) embryos. n = 2 and 3: pooled biological replicates per RNA-Seq library. Genes critical for lymph vessel formation and function are indicated. Red and blue dots indicate downregulated and upregulated genes in Dot1l∆EC LECs, respectively. b qRT-PCR analysis confirming reduced gene expression in E15.5 Dot1l∆EC dermal LECs compared with the wild type (n = 2/group). Error bars show mean ± s.e.m. c, d Gene Ontology (GO) term analysis and Gene Set Enrichment Analysis (GSEA) with genes downregulated in Dot1l∆EC LECs. Each red (repressed genes in cKO) and blue (upregulated genes in cKO) represents one gene in each GO term, respectively. NES normalized enrichment score, FDR false discovery rate.

Back to article page