Fig. 3: The emerging role of BRD4 in the angiogenic progression of GIST cells. | Cell Death & Disease

Fig. 3: The emerging role of BRD4 in the angiogenic progression of GIST cells.

From: BRD4 promotes tumor progression and NF-κB/CCL2-dependent tumor-associated macrophage recruitment in GIST

Fig. 3

a GIST882 and GIST-T1 cells stably expressing vector or BRD4 were harvested, and expression of the indicated proteins was examined by Western blotting. b GIST882 and GIST-T1 cells stably expressing vector or BRD4 were cultured with DMEM without serum overnight. Conditioned media were collected for ELISA assays to assess VEGFA secretion. c GIST882 and GIST-T1 cells stably expressing shcon or shBRD4 were harvested and expression of the indicated protein was examined by Western blotting. d GIST882 and GIST-T1 cells stably expressing shcon or shBRD4 were cultured with DMEM without serum overnight. Conditioned media were collected for ELISA assays to assess VEGFA secretion. e Conditioned media from GIST882 cells stably expressing vector or BRD4 were incubated with HUVECs cells in 96-well plates. The tube formation was examined by the branching points per filed. f Conditioned media from GIST-T1 cells stably expressing shcon or shBRD4 were incubated with HUVECs cells in 96-well plates. Tube formation was examined based on the number of branching points per field. Scale bar: 100 μm; **P < 0.01; ***P < 0.001.

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