Fig. 2: BRD4 promotes GIST cell proliferation, migration, and invasion. | Cell Death & Disease

Fig. 2: BRD4 promotes GIST cell proliferation, migration, and invasion.

From: BRD4 promotes tumor progression and NF-κB/CCL2-dependent tumor-associated macrophage recruitment in GIST

Fig. 2

a GIST882 and GIST-T1 cells stably expressing vector or BRD4 were harvested. BRD4 protein expression was examined by western blotting. b The viability of GIST882 and GIST-T1 cells stably expressing vector or BRD4 was detected by CCK8 assay. c DNA synthesis was examined in cells stably expressing vector and cells stably expressing BRD4 using BrdU/PI assays. d GIST882 and GIST-T1 cells stably expressing vector or BRD4 were seeded on six-well plates for colony formation assays. e and f Both GIST882 and GIST-T1 cells stably expressing vector or BRD4 were plated in the upper chamber with/without Matrigel for 24 h. Then, cell migration and invasion were assessed by observing the cells that migrated to the underside of the transwell insert. Scale bar: 25 μm. g Wound healing assays were used to examine cell migration in GIST882 cells stably expressing vector or BRD4. h Wound healing assays were used to examine cell migration in GIST-T1 cells stably expressing vector or BRD4; *P < 0.05; **P < 0.01.

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