Fig. 2: Expression pattern of transcription factors. | Cell Death & Disease

Fig. 2: Expression pattern of transcription factors.

From: Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors

Fig. 2

a Schematic representation of involvement of transcription factors Lmx1a, Nurr1 and Pitx3 in patterning of hiPSCs to dopaminergic neurons. b Experimental design for the use of viral vector to pattern hiPSCs to generate dopaminergic neurons. cf Quantitative analysis of mRNA expression of infected rat transcription factors (rTFs) (c), endogenous human transcription factors (hTFs) (d), expression of endogenous hLmx1a in hiPSCs after transducing hiPSCs with AAV-HBA-rNurr1 and AAV-HBA-rPitx3-AU1 (e) and expression of endogenous human transcription factors (hTFs) after transducing hiPSCs with AAV-HBA-EGFP (f). Data represented as fold change ± SD as compared to their respective untreated control at the respective time point from three independent differentiations for each transcription factor.

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