Hepatoblastoma (HB) is the most common hepatic neoplasm in childhood and the therapeutic outcomes remain undesirable due to its recurrence and metastasis. Increasing evidence shows that dipeptidase 1 (DPEP1) has pivotal function in tumorigenesis in multiple tumors. However, the expression pattern, biological function, and underlying mechanism of DPEP1 in HB have not been reported. Here we showed that DPEP1 was significantly upregulated and was associated with poor prognosis in HB patients. In vitro and in vivo assays indicated that silencing DPEP1 significantly suppressed HB cell proliferation, migration, and invasion, while DPEP1 overexpression exhibited the opposite effect. In addition, we identified that DPEP1 was a direct target of microRNA-193a-5p (miR-193a-5p). Functional experiments demonstrated that overexpression of miR-193a-5p significantly inhibited cell proliferation and invasion of HB cells, while the inhibitory effect could be reversed by DPEP1 overexpression. Moreover, miR-193a-5p was decreased in HB tumor tissues and associated with a poor clinical prognosis. Mechanistically, our results indicated that the miR-193a-5p/DPEP1 axis participated to the progression of HB via regulating the PI3K/Akt/mTOR (phosphatidylinositol-3-kinase/Akt/mammalian target of rapamycin) signaling. In conclusion, our findings suggest that the miR-193a-5p /DPEP1 axis might be a good prognostic predictor and therapeutic target in HB.
Hepatoblastoma (HB) is one of the most highly invasive malignant carcinoma in children, which accounts for ~50% of pediatric liver cancers1,2. However, about 20% of those patients already have metastasis at the first diagnosis3. Despite regional and local control of HB have improved owing to the application of adjuvant chemotherapy, surgical resection, and liver transplantation, the prognosis for patients in advanced HB stages remains very poor4,5. Thus, it is particularly important to identify valid biomarkers for early diagnosis and treatment of HB.
Dipeptidase 1 (DPEP1) is a zinc-dependent metalloproteinase, and participates in the metabolism of glutathione and other similar compounds by dipeptide hydrolysis6. Recently, more attention has been attracted to the role of DPEP1 in human malignancies. DPEP1 is frequently dysregulated in many tumors, including colorectal cancer, colon cancer, and breast cancer7,8,9. However, the expression pattern and function of DPEP1 in HB is not clear.
MicroRNAs (miRNAs) are a class of small noncoding RNAs with ~22 nucleotide that can regulate the targeted gene expression by binding to the 3′-untranslated region (UTR) of their target messenger RNAs (mRNAs)10. Increasing evidence has found that miRNAs play a critical role in the tumorigenesis of most human malignancies, including HB11,12. Various miRNAs have been studied in the tumorigenesis and metastasis of HB, such as miR-21, miR-17, miR-492, miR-124, and miR-20612,13,14,15. MiR-193a-5p has been investigated as a tumor suppressor in multiple cancers16,17,18,19. However, the specific function of miR-193a-5p in HB tumorigenesis and progression is unknown.
In this current study, we found the upregulated expression of DPEP1 in HB patient tumor tissues and HB cell lines. Silencing DPEP1 inhibited HB cell proliferation, migration, and invasion in vitro, and inhibited HB tumor development in vivo. Further, we identified that miR-193a-5p directly targeted DPEP1 by binding to its 3′-UTR. Cell proliferation and invasion capacity were inhibited by miR-193a-5p overexpression, which could be reversed by the overexpression of DPEP1. We also demonstrated that high expression of DPEP1 or low expression of miR-193a-5p was remarkably related to poor prognosis in HB patients. Moreover, we identified that miR-193a-5p/DPEP1 axis was critical in regulating PI3K/Akt/mTOR (phosphatidylinositol-3-kinase/Akt/mammalian target of rapamycin) signaling. Taken together, our results indicate miR-193a-5p/DPEP1 as a novel regulatory axis in HB, which could be utilized as an effective therapeutic target in HB.
DPEP1 is upregulated in HB tissues and high DPEP1 expression correlates with poor prognosis in HB patients
To explore DPEP1 expression pattern in different cancers, we analyzed the expression levels of DPEP1 mRNA in various types of cancers using The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEX) database. The results suggested that DPEP1 was highly expressed in most cancers (Fig. 1a). Meanwhile, the analysis of Gene Expression Omnibus (GEO) database (GSE75271) revealed that DPEP1 was drastically upregulated in HB tissues in comparison with that in non-tumor control tissues at the mRNA level (Fig. 1b). To further confirm the expression of DPEP1 protein in HB, immunohistochemistry (IHC) was performed on HB tissue microarrays (TMAs). We used a scoring system based on the IHC staining intensity of DPEP1 (Fig. 1c). Consistently, DPEP1 expression was markedly enhanced in HB tissues in comparison with that in normal control tissues (Fig. 1d).
Furthermore, we found that high DPEP1 expression was associated with vascular invasion, distant metastasis, recurrence, and SIOPEL + GPOH (International Society of Pediatric Oncology – Liver Tumor Strategy Group+German Society for Pediatric Oncology and Hematology) risk stratification (Fig. 1e–h and Table 1). Kaplan–Meier analysis also demonstrated that overall survival (OS) was markedly reduced in HB-afflicted children with higher DPEP1 expression (Fig. 1i). Consistently, Gene Set Enrichment Analysis (GSEA) suggested that high expression level of DPEP1 was correlated with poor prognosis (Fig. 1j). Univariate and multivariate Cox regression analysis revealed that vascular invasion, distant metastasis, recurrence, and high DPEP1 expression were independent risk factors for survival in children with HB (Fig. 1k and Table 2). Taken together, these findings suggest that DPEP1 might play a vital function in the tumorigenesis of HB.
Knockdown of DPEP1 suppresses HB cell proliferation, migration, and invasion in vitro
To investigate the biological function of DPEP1 in HB cells, we examined the DPEP1 expression in normal liver cells (L02 and Chang Liver) and HB cell lines (HepG2 and HuH-6). Western blot result showed that the expression levels of DPEP1 were much higher in HepG2 and HuH-6 cells than that in normal liver cells (Fig. 2a). Next, we employed small interfering RNA (siRNA) to knockdown DPEP1 expression in HepG2 and HuH-6 cells (Fig. 2b and Supplementary Fig. S1). Then, DPEP1 immunofluorescence analysis was performed. DPEP1 protein expression was mainly localized in the cytoplasm of HB cells. The results of the DPEP1 immunofluorescence assay further confirmed the significantly downregulated expression of DPEP1 after transfection with DPEP1 siRNA (Fig. 2c). Cell Counting Kit-8 (CCK-8) proliferation assay (Fig. 2d), 5-ethynyl-2′-deoxyuridine (EDU) staining (Fig. 2e), and colony formation assays (Fig. 2f) demonstrated that cell proliferation ability was dramatically suppressed after silencing DPEP1. In addition, the migratory and invasive ability were also remarkably decreased in HepG2 or HuH-6 cells transfected with DPEP1 siRNA, as demonstrated by wound-healing (Fig. 2g) and transwell assays (Fig. 2h). GSEA and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the levels of DPEP1 expression were positively correlated with DNA repair, G2M checkpoint, recombination, or cell cycle (Fig. 2i). On the contrary, we demonstrated that upregulation of DPEP1 promoted HB cell proliferation, migration, and invasion in vitro (Supplementary Fig. S2). Overall, we concluded that DPEP1 could promote HB cell proliferation, migration, and invasion.
Knockdown of DPEP1 inhibits tumor formation in vivo
To verify the effect of DPEP1 downregulation on HB tumorigenesis in vivo, HepG2 cells with stable DPEP1 knockdown (transfected with DPEP1 shRNA (short hairpin RNA)) or mock control (transfected with MOCK-shRNA) were implanted into nude mice. Knockdown of DPEP1 significantly inhibited tumor growth as shown by luciferase photon flux and tumor volumes at different time points after implantation (Fig. 3a–c). Consistently, the tumors in sh-DPEP1 group had markedly lower tumor weight compared with those in mock control group (Fig. 3d). We further examined the proliferation marker Ki-67 and found its expression was also decreased in the sh-DPEP1 group by IHC analysis (Fig. 3e, f).
To further confirm the above results in a system that more accurately mimic the in vivo environment, an orthotopic HB transplantation model in C57BL/6 mice was established using subcutaneous tumor of nude mice. Fluorescence images demonstrated that DPEP1 knockdown resulted in significantly decreased tumor size (Fig. 3g, h). The orthotopic xenograft tumors in sh-DPEP1 group had less tumor metastasis and lower liver weight/body weight ratios (Fig. 3i, j). These results further indicate the oncogenic ability of DPEP1 in HB.
DPEP1 activates PI3K/Akt/mTOR signaling in HB
To further study the potential function mechanism of DPEP1 in HB, bioinformatics analysis was performed based on GEO HB cohort. Heatmap of different gene expression analysis was shown in Supplementary Fig. S3a. KEGG enrichment analysis disclosed several enriched signaling pathways (Fig. 4a). Gene set variation analysis (GSVA) and GSEA indicated that the mTORC1 signaling pathway served as one of the major enriched pathways with high DPEP1 expression (Fig. 4b, c). We found that the protein expression levels of phosphorylated (p)-PI3K, p-AKT, and p-mTOR were significantly decreased in DPEP1-knockdown group and increased in DPEP1 overexpression group, respectively (Fig. 4d). Furthermore, LY294002 (a PI3K signaling inhibitor) was utilized to inhibit the PI3K/Akt/mTOR pathway and the results demonstrated that DPEP1 downregulation could further enhance the inhibitory effect of LY294002, while DPEP1 overexpression could reverse the inhibitory effect of LY294002 in HepG2 (Fig. 4e, f). Similar results were observed in HuH-6 (Supplementary Fig. S3b, c). These results corresponded to the proliferation and invasion abilities of HepG2 cells (Supplementary Fig. S3d). IHC staining of DPEP1 was inversely associated with the IHC scores of mTOR and Akt in HB tissues (Fig. 4g, h).To make our conclusion more solid and rigorous, insulin-like growth factor 1 (IGF-1) was utilized to agonist the PI3K/Akt/mTOR pathway and the results demonstrated that DPEP1 downregulation could reverse the agonist effect of IGF-1 in HepG2 (Supplementary Fig. S4a). Furthermore, rescue experiments in vitro found that activating PI3K/Akt/mTOR pathway using IGF-1 reverted the suppressive effects of DPEP1 silencing on the proliferation and invasion abilities of HepG2 cells (Supplementary Fig. S4b). In addition, we also examined whether DPEP1 regulated the Wnt/β-catenin signaling pathway. GSEA analysis and western blot showed that there was no significant correlation between the expression of Wnt/β-catenin signaling protein and DPEP1 expression (Supplementary Fig. S5). Overall, our results demonstrate that DPEP1 exerts its function via activating PI3K/Akt/mTOR signaling pathway in HB.
DPEP1 is a direct target of miR-193a-5p in HB
To explore the target miRNAs of DPEP1 in HB, we searched for potential target miRNAs by bioinformatics with publicly available databases: miRBase (http://www.mirbase.org/) and TargetScan (http://www.targetscan.org/). Bioinformatics analysis demonstrated that miR-193a-5p had a complementary binding sites targeting 3′-UTR of DPEP1 (Fig. 5a). Western blot and quantitative real-time PCR (qRT-PCR) results indicated the expression levels of miR-139a-5p were negatively correlated with DPEP1 expression in HepG2 and HuH-6 cells. While overexpression miR-193a-5p inhibited DPEP1 expression, inhibition of miR-193a-5p significantly enhanced DPEP1 expression (Fig. 5b, c). Further, the relative luciferase activity in HEK293 cells transfected with the reporter vector containing wild-type (WT) 3′-UTR of DPEP1 was markedly reduced compared to that in HEK293 cells transfected with reporter vector containing mutated 3′-UTR of DPEP1, indicating the direct interaction between miR-193a-5p and 3′-UTR of DPEP1 mRNA (Fig. 5d). Moreover, we confirmed that miR-193a-5p was markedly low expressed in tumor tissues through HB miRNA microarray analysis (GSE75283), which was further validated in our own fresh HB tissue specimens through qRT-PCR analysis (Fig. 5e, f). In addition, the Pearson’s correlation coefficient analysis showed that there was a remarkably negative correlation between miR-193a-5p and DPEP1 expression (Fig. 5g). In situ hybridization (ISH) staining of miR-193a-5p was inversely associated with the IHC scores of DPEP1 in HB tissues (Fig. 5h). In summary, miR-193a-5p directly targets DPEP1 and negatively regulates its expression.
Overexpression of miR-193a-5p suppresses HB cell proliferation and invasion through targeting DPEP1
To demonstrate the effects of miR-193a-5p/DPEP1 axis on HB cells, we transfected HepG2 or HuH-6 cells with miRNA negative control (NC), miR-193a-5p mimics, or miR-193a-5p mimics and DPEP1 plasmid. Western blot exhibited that while miR-193a-5p significantly inhibited DPEP1 expression, DPEP1 overexpression partially rescued DPEP1 expression in miR-193a-5p mimics and DPEP1 plasmid group (Fig. 6a). CCK-8, EDU staining, and colony formation experiments demonstrated that miR-193a-5p overexpression suppressed proliferative potential in both HepG2 and HuH-6 cells, but this suppression effect could be partially reversed by overexpressing DPEP1 (Fig. 6b–f). Meanwhile, DPEP1 overexpression partially rescued the cell invasion of HepG2 or HuH-6 cells with miR-193a-5p overexpression (Fig. 6g).
We further evaluated the function of miR-193a-5p/DPEP1 axis on PI3K/Akt/mTOR signaling. The results suggested that miR-193a-5p mimics inhibited the activation of the PI3K/Akt/mTOR signaling and DPEP1 overexpression could reverse the inhibitory effect (Fig. 6h, i). Taken together, these findings demonstrate that miR-193a-5p suppresses HB cell proliferation and invasion in vitro through targeting DPEP1, which involves the PI3K/Akt/mTOR signaling pathway.
Low miR-193a-5p expression is associated with poor prognosis in HB patients
We analyzed miR-193a-5p expression in an HB TMA cohort by ISH. According to the different staining intensities, we scored the staining intensity of miR-193a-5p with four different levels (1+ to 4+), as shown in Fig. 7a. The results showed that miR-193a-5p was dramatically downregulated in HB tissues in comparison with that in non-tumor tissues (Fig. 7b). In addition, miR-193a-5p expression was associated with vascular invasion, distant metastasis, recurrence, and SIOPEL+ GPOH risk stratification (Fig. 7c–f, Table 1). Furthermore, Kaplan–Meier analysis concluded that low levels of miR-193a-5p related to poor OS rates in HB patients (Fig. 7g). Meanwhile, univariate Cox analysis of OS demonstrated that vascular invasion, distant metastasis, recurrence, SIOPEL + GPOH risk stratification, and miR-193a-5p were important prognostic factors (Fig. 7h). In summary, these results suggest that miR-193-5p is critical for the prognosis of HB.
Emerging evidences have shown that DPEP1 is highly expressed and plays vital roles in the progression of multiple tumors. For instance, previous study has reported that the high expression level of DPEP1 was associated with poor prognosis of colorectal cancer7, and Park et al.8 reported that DPEP1 enhanced colon cancer metastasis by controlling E-cadherin plasticity. Consistent with these results, our data indicated that DPEP1 had an ectopic overexpression in HB tissues and was associated with poor prognosis in HB afflicted children (Fig. 1). In addition, functional assays revealed that DPEP1 silencing could significantly suppress the HB cell proliferative, colony formation, migration, and invasion capacity in vitro, and knockdown of DPEP1 in vivo resulted in decreased HB tumor growth (Figs. 2 and 3). In contrast, DPEP1 expression appears downregulated in pancreatic ductal adenocarcinoma, and the overexpression of DPEP1 inhibited tumor cell invasiveness and acted as a potential tumor suppressor20. Toiyama et al.6 reported that overexpression of DPEP1 inhibited cancer cell invasiveness in the early stages of colon carcinogenesis6. The above-mentioned reports suggest that DPEP1 may exert an oncogene or tumor suppressor function depending on the circumstances and tissue-specific expression pattern.
Subsequently, we verified DPEP1 was targeted by miR-193a-5p (Fig. 5). Previous researches have elucidated that miR-193a-5p played a tumor suppression role in many cancer types. For example, miR-193a-5p overexpression could suppress breast cancer proliferation and metastasis21. MiR-193a-5p could suppress tumor proliferation and enhance radio-sensitivity in esophageal squamous cell carcinoma22. Yu et al.23 concluded that miR-193a-5p could inhibit lung cancer metastasis. Additionally, previous publications had documented that miR-193a-5p was related to chemotherapy drug resistance18. Consistent with these findings, our data indicated that miR-193a-5p was expressed at low levels and was significantly associated with poor clinical outcomes of HB-afflicted children (Fig. 7). In addition, cell proliferative and invasive capacities were inhibited by miR-193a-5p overexpression, which could be reversed by the overexpression of DPEP1 (Fig. 6). Thus, these findings uncovered that miR-193a-5p had a low expression in HB, and miR-193a-5p overexpression could inhibit progression of HB by targeting DPEP1.
Bioinformatics analysis and experimental verification confirmed that DPEP1 acted as an oncogene in HB by activating PI3K/Akt/mTOR signaling pathways (Fig. 4). PI3K/Akt/mTOR signaling is widely considered as a central adjustment factor of cell proliferation, survival, and metabolism24. A great number of reports have shown that PI3K/Akt/mTOR signaling plays a pivotal role in tumorigenesis, including HB25,26. For instance, tamoxifen down-regulating survivin expression in HB cell line HepG2 was mediated by PI3K/Akt/mTOR to induce apoptosis27. Li et al.28 claimed that downregulation of the PI3K/Akt/mTOR signaling could induce apoptosis in HB cancer HepG2 cells. Zhang et al.29 also showed that PP7 could inhibit PI3K/AKT/mTOR signaling and induce autophagic cell death in HepG2. In our study, overexpression of DPEP1 was positively correlated with the expression levels of PI3K/Akt/mTOR signaling-associated molecules (Fig. 4d–f), while miRNA-193a-5p mimics suppressed the activation of the PI3K/AKT/mTOR signaling. Moreover, DPEP1 overexpression could reverse the inhibitory effect of miRNA-193a-5p (Fig. 6h, i). Overall, our results provide solid evidences that miR-193a-5p/DPEP1 is involved in HB progression through controlling the PI3K/Akt/mTOR signaling (Fig. 8).
In summary, we report that miR-193a-5p could regulate DPEP1 expression in tumorigenicity and progression of HB via PI3K/Akt/mTOR signaling. The miR-193a-5p/DPEP1 axis may be used as an effective therapeutic and prognostic biomarker for HB-afflicted patients.
Materials and methods
Patients and specimens
The HB tissues and adjacent normal tissues were collected from HB patients (n = 70) recruited into the First Affiliated Hospital of Zhengzhou University between April 2011 and December 2018. TMAs containing 70-paired paraffin-embedded HB specimens (ZZU cohort) were prepared using 1.5-mm-diameter needle puncture samples. The follow-up and clinicopathological data were listed in Table 1. The study was approved by the Ethics Review Committee of the First Affiliated Hospital of Zhengzhou University. All patients were informed and educated of the procedure and provided written consent.
The HB cell lines (HepG2), hepatocellular carcinoma cell lines (Hepa1-6), and embryonic kidney cell lines (HEK293) were acquired from Cell Bank, Shanghai Institute of Cell Biology (Shanghai, China). Human HB cell line Huh-6, normal liver cell line L02, and Chang Liver were purchased from FuHeng Cell Center (Shanghai, China). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) medium supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin/streptomycin (Corning, NY, USA) in a humid incubator with 5% carbon dioxide at 37 °C. Cells were maintained <6 months before the experiments were conducted. The details of these cells were shown in Supplementary Table 1.
Dataset acquisition and process
TCGA database (http://gdc-portal.nci.nih.gov/) and GTEX database (https://gtexportal.org/) were used to explore the expression of DPEP1 in pan-cancers. Two sets of microarrays (GSE75271, GSE75283) were extracted from the GEO database (http://www.ncbi.nlm.nih.gov/geo/) to analyse the expression of DPEP1 and miR-193a-5p in HB cancer tissues and normal tissues (Supplementary Table 2).
IHC staining and in situ hybridization
IHC was carried out as described previously30. Based on the proportion of positive cells and the different staining intensity, we established a semi-quantitatively scoring system, the proportion of positive cells were scored as follows: 0—none, 1+—<25%, 2+—25–50%, 3+—50–75%, and 4+—75–100%. The staining intensity was scored as follows: 0—none, 1+— weak, 2+—medium, and 3+—strong. The total score was calculated by multiplying the two sub-scores, and samples with scores of 0–6 were deemed as low expression and 7–12 scores were classified as high expression. Blind quantification of DPEP1 staining was performed by two independent pathologists.
ISH was conducted using digoxygenin-labeled miR-193a-5p ISH probes obtained from Boster (Wuhan, China). miR-193a-5p expression in HB TMA was detected based on fluorescence. The different intensities and proportion of miR-193a-5p dyeing were divided into four scores: 1+ to 2+ scores were defined as low expression, while 3+ to 4+ scores represented high expression. Supplementary Table 3 lists the information of the antibodies utilized used in this study.
RIPA buffer was utilized to extracted total protein from HB cells. Following extraction, BCA assays (Beyotime, Shanghai, China) were performed to quantify all proteins. Twenty micrograms of protein samples were loaded onto 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Following separation, the samples were transferred from the gel to the nitrocellulose membranes (Millipore, MA, USA) and blocked with 5% bovine serum albumin/PBST for 1 h. The membranes were then incubated with anti-β-actin, DPEP1, or indicated antibodies at 4 °C overnight. After washed with PBST, the secondary antibody incubation was performed for 2 h and the membranes were exposed with the photographic film for visualization. Supplementary Table 3 listed the information of antibodies.
Oligonucleotide and plasmid transfection
DPEP1 siRNAs (si-DPEP1), DPEP1 plasmid (DPEP1), miR-193a-5p mimics, miR-193a-5p inhibitor, and the corresponding NCs were acquired from GenePharma (Shanghai, China). The sequence of these siRNAs was listed in Supplementary Table 4. Cells were transfected using Lipofectamine 2000 (Thermo Fisher, CA, USA) following the manufacturer’s protocols. Western blotting and qPCR were performed at 48–72 h to determine the transfection efficiency.
Quantitative real-time PCR
Total RNA was extracted using Trizol (Life Technologies, CA, USA). TransScript First-Strand cDNA Synthesis SuperMix (TransGen, Beijing, China) was used to generate complementary DNA (cDNA). qRT-PCR reactions were conducted using PowerUp SYBR Green Kit (ABI, Foster City, USA) and QuantStudio 6 System (ABI, Foster City, USA). The relative gene expression was normalized to control using 2−ΔΔCt method.
Cell proliferation and colony formation assays
Cell proliferation was assessed using CCK-8 Kit (Beyotime, China). The DNA synthesis rate was evaluated through Edu staining assay (Ribobio, Guangzhou, China). Cell colony formation ability after DPEP1 silencing was assessed by colony formation assay. Cells transfected with si-DPEP1, miR-193a-5p, miR-193a-5p and DPEP1 plasmid, or NC controls were seeded in 6-well plates and incubated for 2 weeks. Then, cells were fixed with formaldehyde and stained with 0.1% crystal violet. The colonies with more than 50 cells were counted by light microscopy.
Cell migration and invasion assays
Wound-healing assay was conducted to determine the migration ability. HepG2 and HuH-6 cells (5 × 106) were seeded into six-well plates. A 1 mm wide wound was created using a 200-μL sterile tip after 90% confluence was reached. The wounded areas were observed and photographed every 24 h under microscope.
Cell invasion assay was conducted utilizing transwell chambers coated with Matrigel (BD, NJ, USA). Upper chambers were seeded at 1 × 104 transfected cells using serum-free media, and the lower chamber was filled with DMEM with 10% FBS. After 24 h, the invasive cells were fixed, stained, photographed, and quantified.
Both WT and mutant 3′-UTR of the DPEP1 mRNA were cloned into the luciferase reporter vector (psiCHECK-2, Promega, WI, USA). HEK293 cells were co-transfected with miR-193a-5p mimics and either DPEP1-wt or DPEP1-mut vectors. After 48 h, the relative luciferase activity was analyzed using the Dual-Luciferase Reporter Assay System (Promega, WI, USA).
Lentiviral transduction and vector construction
The CDS of human DPEP1 were cloned into pcDNA3.1 (+) to construct DPEP1 overexpression vectors. HepG2 and Hepa1-6 cells were infected with lentivirus containing DPEP1 shRNA or control lentivirus for 4 days and then screened for stably knockdown cells using puromycin (Santa Cruz Biotechnology, Dallas, TX, USA).
Mice experiments were approved by the Animal Health Committee of Zhengzhou University. The nude mice (male, 4–6 weeks old) and C57BL/6 mice (male, 8 weeks old) were purchased from Beijing Vital River Laboratory (Beijing, China). Cells transfected with DPEP1 knockdown (sh-DPEP1) and empty lentivirus control (MOCK) were subcutaneously implanted into the lower flank of nude mice. HepG2 cell lines were selected to establish xenograft model in nude mice. Liver orthotopic transplantation model was established with Hepa1-6 cells. After 1–2 weeks, a mouse model of orthotopic transplantation of the liver was established by removing the subcutaneous tumor derived from Hepa1-6 cells and transplanting 1 mm3 samples into the liver left lobe of the C57BL/6 mouse (8 weeks old). Tumor growth was examined every week. Mice were euthanized at 5 weeks post orthotopic transplantation. Photographs were taken using the IVIS Lumina II system. The tumor tissues were weighed and extracted for further IHC staining.
All statistical analyses were conducted using SPSS (Version 23.0, IBM, WA, USA). Differences between two groups were analyzed by Student’s t test. Clinicopathological characteristics in HB were analyzed by χ2 tests. OS was calculated with Kaplan–Meier curves and log-rank tests. Univariate and multivariate Cox regression analyses were performed to identify the independent prognostic factors. Correlation was performed by Spearman’s rank analysis with GraphPad Prism 7.0 (San Diego, CA, USA). A p value <0.05 was statistically significant.
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The study was supported by National S&T Major Project of China (2018ZX10301201-008), National Key Research and Development Program of China (2018YFC2000500), National Natural Science Foundation of China (81600506 and 81702757), China Postdoctoral Science Foundation (2017M610463 and 2018M632814), National Engineering Laboratory for Internet Medical System and Application open fund project (NELIMSA2018P03), The joint research fund of the First Affiliated Hospital of Zhengzhou University, and Dalian Institute of Chemical Physics Chinese Academy of Sciences (ZGR). The funding bodies had no roles in the design of the study, collection of samples, analysis and interpretation of the data, or in manuscript writing.
X.C.C., X.L. and G.D. conceived and designed the study. X.C.C., X.L., Q.C.H. and J.M.Z. performed experiments. J.H.L., Z.G.R., L.W.L., Y.B.L., Z.F.W. and D.D.Z. collected the clinical samples. J.H.L., Z.G.R. and L.W.L. conducted analysis of publicly available databases. X.C.C., X.L., Y.Z.F., D.Z. and G.D. analyzed and interpreted data. X.C.C., X.L., Q.C.H., D.Z. and G.D. wrote the manuscript. All authors reviewed and approved the manuscript.
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Cui, X., Liu, X., Han, Q. et al. DPEP1 is a direct target of miR-193a-5p and promotes hepatoblastoma progression by PI3K/Akt/mTOR pathway. Cell Death Dis 10, 701 (2019). https://doi.org/10.1038/s41419-019-1943-0