Fig. 5: MiR-760 and miR-215-3p bound to the 3′UTR of IL22RA1 and inhibited IL22RA1 expression. | Cell Death & Disease

Fig. 5: MiR-760 and miR-215-3p bound to the 3′UTR of IL22RA1 and inhibited IL22RA1 expression.

From: LncRNA NR_003923 promotes cell proliferation, migration, fibrosis, and autophagy via the miR-760/miR-215-3p/IL22RA1 axis in human Tenon’s capsule fibroblasts

Fig. 5

a A scatter-plot was used to assess the expression profiles of mRNAs. b Volcano plots were used to identify mRNAs with a ≥ twofold change in expression and a p-value ≤ 0.05. c The hierarchical clustering of mRNAs according to our filtering criteria. d The difference in IL22RA1 expression between control and glaucoma tissues. IL22RA1 was expressed at significantly higher levels in glaucoma tissues. **p < 0.01 vs control. e, f Mutation sequences were introduced into the binding site for miR-760 and miR-215-3p in the 3′UTR of IL22RA1 mRNA. g, h Dual luciferase reporter assays were used to demonstrate that miR-760 and miR-215-3p could bind to the 3′UTR of IL22RA1 mRNA. I IL22RA1 expression was inhibited by the miR-760 and miR-215-3p mimics. **p < 0.01 vs NC

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