Fig. 6: Teriflunomide reverses the anti-apoptotic effect of apogossypol and enhances BH3 mimetic-mediated apoptosis. | Cell Death & Disease

Fig. 6: Teriflunomide reverses the anti-apoptotic effect of apogossypol and enhances BH3 mimetic-mediated apoptosis.

From: Apogossypol-mediated reorganisation of the endoplasmic reticulum antagonises mitochondrial fission and apoptosis

Fig. 6

a HeLa cells were exposed to teriflunomide (200 μΜ) for 1 h, followed by apogossypol (20 μΜ) for another 1 h, and finally to a combination of BH3 mimetics, A-1331852 (0.1 μΜ) and A-1210477 (10 μΜ) for 4 h and assessed for cell death. b Western blots from cells treated in a indicate changes in the extent of processing of caspase-9, −3 and −7 as well the cleavage of the caspase substrate, PARP. c Densitometric analyses of the different proteins from the blots shown in b were performed using ImageJ software. The values for DMSO controls were normalised to 100%, and the densitometry of the rest plotted relative to the intensities of GAPDH (protein loading control) in the corresponding lanes. Graphs were plotted using data from three independent experiments. Error bars = Mean ± SEM. ***p 0.001 and **p 0.005

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