Fig. 5: Teriflunomide and leflunomide prevent ER membrane reorganisation independent of their ability to inhibit DHODH. | Cell Death & Disease

Fig. 5: Teriflunomide and leflunomide prevent ER membrane reorganisation independent of their ability to inhibit DHODH.

From: Apogossypol-mediated reorganisation of the endoplasmic reticulum antagonises mitochondrial fission and apoptosis

Fig. 5

a HeLa cells were exposed to increasing concentrations of teriflunomide or leflunomide for 1 h, followed by apogossypol (20 μΜ) for 4 h and immunostained with BAP31 antibody. b Quantification of the reduction in ER membrane reorganisation was performed by counting ~300 cells from 3 independent experiments. Error bars = Mean ± SEM. ***p ≤ 0.001. c HeLa cells transiently transfected with two different siRNAs against DHODH for 72 h were exposed to apogossypol (20 μΜ) for 4 h, and then immunostained with BAP31 antibody. d HeLa cells were supplemented with excess orotate or uridine (1 mM each), followed by inhibition of DHODH (teriflunomide or leflunomide at 200 μΜ) for 1 h, and a further 4 h with apogossypol (20 μΜ), before immunostaining with BAP31 antibody. Scale bar: 10 μm

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