Fig. 1: Generation and characterization of meso1 CAR T and meso3 CAR T cells. | Cell Death & Disease

Fig. 1: Generation and characterization of meso1 CAR T and meso3 CAR T cells.

From: Modified CAR T cells targeting membrane-proximal epitope of mesothelin enhances the antitumor function against large solid tumor

Fig. 1

a Schematic illustration of the meso1 CAR and meso3 CAR constructs. b Positive ratio of meso1 CAR T and meso3 CAR T cells were detected by flow cytometry. c Statistic analysis of positive rate of CAR T cells. d The protein expression of exogenous CD3ζ was detected by western blotting. e The average copy-number of CAR in meso1 CAR T cells and meso3 CAR T cells was detected by RT-PCR. Data are expressed as the mean ± SD; n = 3; ns p > 0.05, ***p < 0.001

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