Fig. 5: SDT induces PINK1/Parkin-dependent mitophagy and rescues the expression of PINK1. | Cell Death & Disease

Fig. 5: SDT induces PINK1/Parkin-dependent mitophagy and rescues the expression of PINK1.

From: Sonodynamic therapy inhibits palmitate-induced beta cell dysfunction via PINK1/Parkin-dependent mitophagy

Fig. 5

a SDT increased the colocalization of autophagosomes and mitochondria. Autophagosomes were labeled with an autophagy assay kit (blue-green) and mitochondria with MTG (green and changed to red after merge) and nuclei with Hoechst (blue). Scale bar = 10 μm. b SDT increased the colocalization of mitochondria and bilayer vesicles at 0.5 h post SDT. Mitochondria encapsulated by bilayer vesicles are indicated by white arrows, and black arrows indicate secretory granules. Scale bar = 2 μm. c SDT upregulated mitophagy related protein levels. After 0.5 h post SDT, cell lysates and mitochondrial extracts were analyzed by immunoblotting for the levels of PINK1, LC3B, Parkin. β-Actin and COXIV were used as internal controls. n = 4. d SDT restored the expression level of PINK1 mRNA inhibited by PA. Bars = Mean ± SEM, n = 3. e SDT induced the translocation of Parkin from the cytoplasm to mitochondria. Parkin is shown by green and mitochondria by red. Scale bar = 10 μm. Representative images are shown. COXIV Cytochrome c oxidase IV, CsA cyclosporin A, LC-3 microtubule-associated protein 1 light chain 3, MTR MitoRed, PINK1 PTEN-induced putative kinase 1

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