Fig. 4: SDT reduces PA-induced mitochondrial dysfunction. | Cell Death & Disease

Fig. 4: SDT reduces PA-induced mitochondrial dysfunction.

From: Sonodynamic therapy inhibits palmitate-induced beta cell dysfunction via PINK1/Parkin-dependent mitophagy

Fig. 4

a SDT recovered the loss of Δψm induced by PA. After 24 h post SDT, cells were stained with JC-1. Red-fluorescence indicates normal Δψm, green indicates loss of Δψm and blue indicates nuclei. Scale bar = 100 μm. Date are shown in b as the ratio of red-fluorescent cell number to green-fluorescent cell number. n = 4. cf SDT inhibited both intracellular and mitochondrial ROS production. ROS levels were analyzed by flow cytometry at 24 h post SDT. n = 3. g SDT improved mitochondrial morphology. Images were observed with TEM at 24 h post SDT. The white arrows indicate normal mitochondria, the red arrows indicate swollen mitochondria and the black arrows indicate secretory granules. n = 3. Scale bar = 2 μm. Representative images are shown. Bars = Mean ± SEM. TEM transmission electron microscope, Δψm mitochondrial membrane potential

Back to article page