Fig. 3: Mitochondria are potent targets of SDT in beta cells. | Cell Death & Disease

Fig. 3: Mitochondria are potent targets of SDT in beta cells.

From: Sonodynamic therapy inhibits palmitate-induced beta cell dysfunction via PINK1/Parkin-dependent mitophagy

Fig. 3

a ALA-PpIX (red) localizes to mitochondria. Beta cells were preincubated with ALA. Mitochondria were stained with MTG (green) and nuclei with Hoechst 33342 (blue). n = 3. Scale bar is 10 μm. b SDT induced extensive mitochondrial ROS production. Fluorescence intensity of MitoSOX was measured with fluorescence spectrometry in real time while performing SDT. n = 3. MTG, Mito-Tracker Green; NAC, N-acetylcysteine; ROS, reactive oxygen species. c SDT induced mitochondrial singlet oxygen production. Mitochondrial singlet oxygen was stained with Si-DMA (red) and nuclei with Hoechst 33342 (blue). Cells were observed with LSCM at 0.5 h post SDT. n = 3. Scale bar is 30 μm

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