Fig. 4: DC-associated B7-H3 suppresses the production of anti-DNA Abs and renal inflammation. | Cell Death & Disease

Fig. 4: DC-associated B7-H3 suppresses the production of anti-DNA Abs and renal inflammation.

From: Dendritic cell-associated B7-H3 suppresses the production of autoantibodies and renal inflammation in a mouse model of systemic lupus erythematosus

Fig. 4

a BMDC were stimulated with or without LPS and the expression of B7-H3 cell surface protein (left panel) or B7-H3 mRNA (right panel) were determined by flow cytometry with specific B7-H3 mAb (left) or qRT-PCR (right). b BMDC at 106 cells from WT or B7-H3KO mice were pre-incubated with ALD-DNA(5ug/ml) and were intravenously injected into B7-H3KO mice for immunization. The serum was collected from each mouse every two weeks and the anti-dsDNA Abs were detected by specific ELISA. c. BMDC-ALD-DNA at 106 cells were intravenously injected into B6 mice for immunization and subsequently treated with 14 M or control Ig weekly. The serum was collected from each mouse every two weeks and the anti-dsDNA Abs were detected by specific ELISA. df Ten weeks after BMDC-ALD-DNA immunization, kidney sections were stained by hematoxylin and eosin (400×) (d left), the renal score of two groups (d right), IgG immunohistochemically staining (400×) (e) and complement 3 immunohistochemically staining (400×) (f). Results are representatives of five mice

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