Fig. 7: Nonphosphorylated SET isoform 1 is inactive in promoting tumorigenesis in mice. | Cell Death & Disease

Fig. 7: Nonphosphorylated SET isoform 1 is inactive in promoting tumorigenesis in mice.

From: Cyclin-dependent kinase 1-mediated phosphorylation of SET at serine 7 is essential for its oncogenic activity

Fig. 7

a Representative tumors in each group were excised and photographed at the endpoint. b Tumor growth curve with various RKO cell lines. Cells were subcutaneously inoculated into athymic nude mice on both flanks (five mice each group). The tumor volume shown at each point was the average from 6 to 10 tumors (two animals in shSET and shSET+SET-S7A groups did not form any palpable tumors, mean ± SD). ***p < 0.001 (unpaired Student’s t test). c Ki-67 (proliferation) and cleaved caspase-3 (apoptosis) staining in tumors shown in a. Tumors from shSET+ isoform 1 SET-WT and shSET+ isoform 1 SET-S7A groups were analyzed. d IHC staining with p-AKT T308 antibody in tumors shown in a. e Western blotting analysis of SET (isoforms 1 and 2) expression in immortalized colon epithelial (HCEC) and colon cancer cell lines. fj High expression of SET (all isoforms) correlates with poor clinical outcome. Kaplan–Meier curves of overall survival in various cancers. Data were generated from an online analysis tool (http://kmplot.com) using RNA-seq data49

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