Fig. 3: Phosphorylation of SET isoform 1 at S7 is required for proper mitosis in HeLa cells. | Cell Death & Disease

Fig. 3: Phosphorylation of SET isoform 1 at S7 is required for proper mitosis in HeLa cells.

From: Cyclin-dependent kinase 1-mediated phosphorylation of SET at serine 7 is essential for its oncogenic activity

Fig. 3

a Western blotting analysis of HeLa SET-knockdown cells stably expressing Flag-tagged shRNA-resistant (Res) isoform 1 SET-WT or SET-S7A. b, c Knockdown of SET resulted in multinucleated cells. Representative photos of control (shControl) and SET-knockdown (shSET) cells were shown in b. Quantification of multinucleated cells (c) in cell lines established in a. Arrows in b indicate some of the multinucleated cells. Data in c were expressed as the mean ± SD of three independent experiments. ***p < 0.001 (unpaired Student’s t test). A total of 250 cells in each group were counted. d, e Knockdown of SET resulted in massive mitotic defects. Representative photos of normal mitosis (shControl) and mitotic abnormalities (shSET) in HeLa cells were shown. Cells were stained with β-tubulin, γ-tubulin antibodies, and DAPI to visualize microtubules (green), centrosomes (red), and chromosomes (blue), respectively. Data in e were collected from 160 mitotic cells for each group (mean ± SD of three independent experiments). ***p < 0.001 (unpaired Student’s t test)

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