Fig. 2: Gene expression profiling identifies WNT/ß-Catenin and PI3K/AKT signaling pathways as the major targets of SARB hybrid treatment. | Cell Death & Disease

Fig. 2: Gene expression profiling identifies WNT/ß-Catenin and PI3K/AKT signaling pathways as the major targets of SARB hybrid treatment.

From: Combination of 5-fluorouracil and thymoquinone targets stem cell gene signature in colorectal cancer cells

Fig. 2

(A) Scatter plot of the whole NanoString gene set of HCT116 cells after 24 h of treatment with Combi and SARB hybrid. Normalized grouped data are shown. Log10 data are shown as treatment vs. untreated samples (n = 3). Pearson's correlation was calculated using GraphPad Prism 7.0. KEGG pathway was performed using STRING database (version 10.5) on the significantly downregulated genes under (B) Combi and under (C) SARB treatment. (D) Venn diagram of significantly up and (E) downregulated genes upon treatments. A p-value of  ≤ 0.05 and a fold change of  ≥ / ≤ 1.5-fold are selected for the analysis. (F) RT-qPCR validation of WNT target genes and FOS gene in HCT116 cells. Gene expression fold-change differences in Combi and SARB hybrid after 24 h of treatment. Values represent means ± SEM (n = 3), (*p < 0.05; **p < 0.01; ***p < 0.001; One way-ANOVA). B2M was used as a housekeeping gene. (G) Western Blot analysis of key proteins involved in PI3K/AKT signaling in HCT116 cells after 24 h of treatment with TQ 40, 5-FU 15, Combi, and SARB at same concentration of 35 µM. Western Blot images are representative of at least two independent experiments. The quantification values are presented as relative to GAPDH housekeeping protein

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