Fig. 2: B7-H3 promoted the expression of HK2 in CRC cells. | Cell Death & Disease

Fig. 2: B7-H3 promoted the expression of HK2 in CRC cells.

From: B7-H3 promotes aerobic glycolysis and chemoresistance in colorectal cancer cells by regulating HK2

Fig. 2

a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) and RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) and RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level and STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 and RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. β-actin served as a loading control. f HK2 protein level and STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 and RKO cells. β-actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, and the experiments were performed in triplicate. *P < 0.05