Fig. 1: B7-H3 promoted aerobic glycolysis in CRC cells. | Cell Death & Disease

Fig. 1: B7-H3 promoted aerobic glycolysis in CRC cells.

From: B7-H3 promotes aerobic glycolysis and chemoresistance in colorectal cancer cells by regulating HK2

Fig. 1

a B7-H3 protein levels in both B7-H3-overexpressing HCT116 and RKO cells were analyzed by western blot. β-actin served as a loading control. b, c Glucose consumption (b) and lactate production (c) were measured in both B7-H3-overexpressing HCT116 and RKO cells. d B7-H3 protein levels in both HCT116 and RKO cells were analyzed by western blot after transfection with siRNA negative control (NC), B7-H3 siRNA-1 or B7-H3 siRNA-2. β-actin served as a loading control. e, f Glucose consumption (e) and lactate production (f) were measured in both HCT116 and RKO cells transfected with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. Values were expressed as means (SEMs). Five samples were analyzed per condition, and the experiments were performed in triplicate. *P < 0.05

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