Fig. 2: Inhibition of SET8 impaired cell proliferation and reduced glycolysis in HCC cells. | Cell Death & Disease

Fig. 2: Inhibition of SET8 impaired cell proliferation and reduced glycolysis in HCC cells.

From: Monomethyltransferase SET8 facilitates hepatocellular carcinoma growth by enhancing aerobic glycolysis

Fig. 2

a Assessment of cell growth via CCK-8 assay in control (shCTL) and SET8 knockdown (shSET8) M3 (left) and 97H (right) cells. b Migration assay of shCTL and shSET8 cells using a transwell system. Upper, M3 cells; down, 97H cells. c Colony-formation assay performed in shCTL and shSET8 cells, and numbers of colonies were counted 14 days later. d Flow-cytometry analysis using Annexin V and 7AAD staining to determine the apoptosis rate. Upper, M3 cells; down, 97H cells. e, f Analysis of the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in M3 (left) and 97H (right) cells with either control shRNA or SET8 shRNA. g, h The expression levels of genes involved in glucose metabolism in the indicated HCC cell lines with either SET8 knockdown or control shRNA detected by real-time PCR and western blot analysis. i SET8 shRNA-infected cells were transfected with the SET8 plasmid for 48 h, and cells were then subjected to western blot analysis. Data are shown as mean ± SD of three independent experiments. *P < 0.05

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