Fig. 5: Surface glucose-regulated protein 78 (GRP78) acts as a pro-apoptotic signaling receptor. | Cell Death & Disease

Fig. 5: Surface glucose-regulated protein 78 (GRP78) acts as a pro-apoptotic signaling receptor.

From: Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells

Fig. 5

a Relative caspase 3/7 activity in INS-1E cells exposed to cytokines (human interleukin (hIL)-1β (50 U/ml) and rat interferon (rIFN)-γ (500 U/ml)) with or without the addition of anti-GRP78 antibody against the N-terminal region (N-term, 5 μg/ml, left panel. n = 5) or C-terminal region (C-term, 10 μg/ml, right panel, n = 5) for 16 h. bf Relative mRNA expression of pro-apoptotic markers Chop (b), Dp5 (c), Atf3 (d), and Bax (e) and the anti-apoptotic marker Mcl-1 (f) in control and cytokine-exposed INS-1E cells (hIL-1β (50 U/ml), rIFN-γ (500 U/ml)) with or without the addition of anti-GRP78 antibody against the N-terminal (N-term, 5 μg/ml, left panels, n = 3) or C-terminal (C-term, 10 μg/ml, right panels, n = 5) region for 16 h. Data are presented as mean ± SEM and statistically analyzed by a one-way analysis of variance followed by Sidak posthoc test for multiple group comparisons. Significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001

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