Fig. 7: Kynurenine and teriflunomide treatment induces apoptotic cell death. | Cell Death & Disease

Fig. 7: Kynurenine and teriflunomide treatment induces apoptotic cell death.

From: Tryptophan-kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection

Fig. 7

a Infected cells were treated with either kynurenine or teriflunomide, and at indicated time points, cell viability was measured by trypan blue. Error bars show mean values ± SE of three repeated experiments. * and # represent statistically significant (p < 0.05), comparison was made relative to cell, only infected with parasite at the corresponding time point. b Genomic DNA was isolated from T. gondii infected cells, which were treated with or without kynurenine or teriflunomide separately and pattern of DNA fragmentation was shown by agarose gel electrophoresis. c T. gondii infected cells, which were treated with or without kynurenine or teriflunomide separately, and total cell-lysates at indicated time was collected for immunoblotting using phospho-AKT (60 kDa), phospho-β-catenin (92 kDa) and caspase-3 antibody (35 kDa, 17 kDa). The blot is the representation of three separate experiments

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