Fig. 6: ROS impedes T. gondii replication, followed by small molecule treatment. | Cell Death & Disease

Fig. 6: ROS impedes T. gondii replication, followed by small molecule treatment.

From: Tryptophan-kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection

Fig. 6

a Cells were incubated with tBHP, or NAC, or melatonin or infected with parasite (MOI 2) and released H2O2 from cells were measured spectrophotometrically, and viability of cells were determined by trypan blue. b Cells were incubated with NAC, or melatonin, or tBHP, followed by infection with parasite. At indicated time points, released H2O2 was measured. Data showed average values ± SE of three repeated experiments. and # represent statistically significant (p < 0.05), comparison was made relative to cells infected with parasite only. c Infected cells were treated with either NAC, or melatonin, or tBHP and immunoblot was done using SAG1 antibody to check the parasite growth. To calculate the number of intracellular parasites and their ability to develop PVs, confocal microscopy was done using mCherry expressing parasites in similar conditions. T. gondii infected cells were treated with d kynurenine and e teriflunomide. The growth of parasites was checked by both qPCR and the immunoblot as well as by confocal microscopy. Bar diagram denotes the mean ± SE of four independent experiments. * highlights statistically significant (p < 0.01), each time point compared with same time point of infected cells without any treatment

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