Fig. 4: Effect of CGP-37157 and siNCX1 on mitochondrial Ca2+ levels in SH-SY5Y differentiated cells exposed to α-synuclein (α-syn) and rotenone (Rot). | Cell Death & Disease

Fig. 4: Effect of CGP-37157 and siNCX1 on mitochondrial Ca2+ levels in SH-SY5Y differentiated cells exposed to α-synuclein (α-syn) and rotenone (Rot).

From: Selective inhibition of mitochondrial sodium-calcium exchanger protects striatal neurons from α-synuclein plus rotenone induced toxicity

Fig. 4

a Representative images of SH-SY5Y differentiated cells loaded with Rhod 2-AM before (left) and after Rot+α-syn application (right). b Histogram showing mitochondrial Ca2+ levels expressed as Δ% fluorescence increases under resting conditions (open bars) and during 3 nM α-syn + 0.3 µM Rot applied alone or in combination with 3 µM CGP-37157 (filled bars). For each experimental group (control, CGP-37157, α-syn, α-syn+Rot, CGP-37157+α-syn+Rot), Δ% values used for the statistical analysis derived from 4 independent experiments and 100–200 cells were recorded in each different session (*P < 0.0001, α-syn+Rot vs all groups; #P < 0.001, CGP-37157+α-syn+Rot vs α-syn+Rot; CGP-37157+α-syn+Rot vs control; CGP-37157+α-syn+Rot vs α-syn). c Representative recordings of mitochondrial Ca2+ responses in control conditions (black line), in the presence of α-syn+Rot (green line) co-applied with 3 µM of CGP-37157 (orange line). d Histogram showing mitochondrial Ca2+ levels expressed as Δ% fluorescence increases in SH-SY5Y control cells, in SH-SY5Y cells treated with siNCX, in cells treated with α-syn+Rot, and in cells silenced with siNCX and α-syn+Rot. For each experimental group Δ% values used for the statistical analysis derived from 6 independent experiments and 100–200 cells were recorded in each different session (*P < 0.0001, α-syn+Rot vs. all groups; #P < 0.001 siNCX α-syn+Rot vs all groups). e Representative recordings of mitochondrial Ca2+ responses in control conditions (black line), in the presence of α-syn+Rot (blue line), and α-syn+Rot in cells silenced with siNCX1 (cyan line)