Fig. 3: Effect of CGP-37157 on mitochondrial Ca2+ levels in striatal slices exposed to α-synuclein (α-syn) and rotenone (Rot). | Cell Death & Disease

Fig. 3: Effect of CGP-37157 on mitochondrial Ca2+ levels in striatal slices exposed to α-synuclein (α-syn) and rotenone (Rot).

From: Selective inhibition of mitochondrial sodium-calcium exchanger protects striatal neurons from α-synuclein plus rotenone induced toxicity

Fig. 3

a Representative image of a striatal slice showing neurons loaded with Rhod 2-AM. b Histogram showing mitochondrial Ca2+ levels expressed as percentage of the fluorescence change increase (ΔF/F%) under resting conditions (open bars) and during 3 nM α-syn + 0.3 µM Rot applied alone or in combination with 3 µM CGP-37157 (filled bars). For each experimental group, Δ% values used for the statistical analysis derived from 4 independent experiments and 50–100 cells were recorded in each different session (*P < 0.001 α-syn+Rot vs all groups, #P < 0.001 CGP-37157+α-syn+Rot vs α-syn+Rot). c Representative recordings of mitochondrial Ca2+ responses in control conditions (black line), in the presence of α-syn+Rot (blue line) and in the presence of α-syn+Rot co-applied with 3 µM CGP-37157 (red line). Fluorescence intensity values were normalized to resting fluorescence (F/F0)