Fig. 4: Effect of hsa_circ_001783 on MDA-MB-231 and MDA-MB-468 progression. | Cell Death & Disease

Fig. 4: Effect of hsa_circ_001783 on MDA-MB-231 and MDA-MB-468 progression.

From: Circular RNA hsa_circ_001783 regulates breast cancer progression via sponging miR-200c-3p

Fig. 4

a Schematic representation of the siRNA sequences specifically targeted the junction site of hsa_circ_001783. b qPCR analysis of the transfection efficacy and off-target effects of siRNA on EBLN3 and ZCCHC7 expression in MDA-MB-231 and MDA-MB-468 after 24 h transfection. **P < 0.01 compared to mock; *P < 0.05 compared to mock. c The proliferation status of MDA-MB-231 and MDA-MB-468 determined by CCK-8 assay after hsa_circ_001783 knockdown. OD optical density. **P < 0.01 compared to mock. d EdU assay to determine DNA synthesis of MDA-MB-231 and MDA-MB-468 transfected with siRNAs. All the data are shown as the mean ± SD; *P < 0.05 compared to mock. Scale bar, 100 μm. e Colony formation ability of MDA-MB-231 and MDA-MB-468 transfected with siRNA. All the data are shown as the mean ± SD; *P < 0.05 compared to mock. f The representative images of migrated MDA-MB-231 and MDA-MB-468 after hsa_circ_001783 knockdown. All the data are shown as the mean ± SD; *P < 0.05, compared to mock. Scale bar, 100 μm. g The representative images of invaded MDA-MB-231 and MDA-MB-468 after hsa_circ_001783 knockdown. All the data are shown as the mean ± SD; *P < 0.05, compared to mock. Scale bar, 100 μm

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