Fig. 1: Screening circRNA candidates in breast cancer and characteristics of hsa_circ_001783. | Cell Death & Disease

Fig. 1: Screening circRNA candidates in breast cancer and characteristics of hsa_circ_001783.

From: Circular RNA hsa_circ_001783 regulates breast cancer progression via sponging miR-200c-3p

Fig. 1

a Workflow of screening circRNA candidates in breast cancer. b The biological process ontology terms of hsa_circ_001783. c The genomic loci of hsa_circ_001783 and the Sanger sequence of junction site of hsa_circ_001783 in two breast cancer cell lines: MDA-MB-231 and MDA-MB-468. d qPCR analysis of β-actin, GAPDH, and hsa_circ_001783 (1783) after Rnase R treatment. ***P < 0.001 compared to no treatment group. e qPCR analysis of poly A-tailed mRNAs including β-actin and GAPDH which can be synthesized to cDNA by using random primer or oligo DT primer alone and non-poly A-tailed RNA hsa_circ_001783 (1783) which cannot be reversed to cDNA by using oligo DT primer. ***P < 0.001 compared to the random primer. f qPCR analysis of hsa_circ_001783 in the cytoplasm and nuclear of which was separated by PARIS kit. β-Actin mRNA in cytoplasm and long non-coding RNA malat1 residing in nuclear fraction were referred as quality controls of nuclear and cytoplasm fractions, respectively. Total RNA was total portion of the nuclear plus cytoplasm and was referred as the control. ***P < 0.001 compared to the cytoplasm

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