Fig. 3: Overexpression of P2X7R in PDLSCs (adenovirus transduction). | Cell Death & Disease

Fig. 3: Overexpression of P2X7R in PDLSCs (adenovirus transduction).

From: Role of the P2X7 receptor in inflammation-mediated changes in the osteogenesis of periodontal ligament stem cells

Fig. 3

a P2X7R mRNA expression in PDLSCs (assessed by qRT-PCR) in response to transduction with Ad-control or Ad-P2X7 at 10 pfu/cell (MOI = 10) or 20 pfu/cell (MOI = 20); cells without transduction were used as the control group. b P2X7R protein expression in PDLSCs (assessed by western blotting) in response to transduction with Ad-control or Ad-P2X7 at 10 pfu/cell (MOI = 10) or 20 pfu/cell (MOI = 20); cells without transduction were used as the control group. c Proliferative activity of Ad-P2X7-transfected PDLSCs (MOI = 20) and Ad-control-transfected PDLSCs assessed by the CCK-8 assay. d Representative images (confocal micrograph) of PDLSCs transfected with Ad-P2X7 or Ad-control (MOI = 20), which carries the green fluorescence protein (GFP) gene and P2X7R (immunofluorescence staining with a P2X7R antibody, red) (scale bar = 20 μm). Data are presented as the mean ± S.D. for n = 3; ns indicates no significant difference between the indicated columns; *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significant differences between the indicated columns

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