Fig. 6: Effect of Euro 5 and Euro 4 DEPs, before any after-treatment, on PAD enzymatic activity induction. | Cell Death & Disease

Fig. 6: Effect of Euro 5 and Euro 4 DEPs, before any after-treatment, on PAD enzymatic activity induction.

From: Diesel exhaust particles induce autophagy and citrullination in Normal Human Bronchial Epithelial cells

Fig. 6

PAD activity was assessed in lysates a from NHBE cells (i) untreated and cultured for 48 h with (ii) Euro 5 3.3 μg/cm2, (iii) Euro 4 3.3 μg/cm2, (iv) Euro 5 6.6 μg/cm2, (v) Euro 4 6.6 μg/cm2. Quadruplicate analyses (replicates) were conducted for all the lysates in three different assays (N = 3) and results obtained are shown as mean ± SD. For 3.3 μg/cm2: P = 0.0344 Euro 5 vs untreated; P = 0.0203 Euro 4 vs untreated. For 6.6 μg/cm2: P = 0.0051 Euro 5 vs untreated; P = 0.0025 Euro 4 vs untreated. For a dose-depending comparison: P = 0.0422 Euro 5 3.3 vs 6.6 μg/cm2; P = 0.0161 Euro 4 3.3 vs 6.6 μg/cm2. b Since Ca2 + is required for PAD activity, the background of the assay was set up by analyzing each lysate in the presence of the chelating agent EDTA. No increase in PAD activity was observed, following treatment with Euro 4 and Euro 5 DEPs (NS, P > 0.05 for all the experimental conditions). Quadruplicate analyses (replicates) were conducted for all the lysates in three different assays (N = 3) and results obtained are shown as mean ± SD

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